ID   MDFIC_XENLA             Reviewed;         246 AA.
AC   Q98SK0;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=MyoD family inhibitor domain-containing protein;
DE   AltName: Full=I-mfa domain-containing protein;
DE   AltName: Full=XIC;
GN   Name=mdfic {ECO:0000250|UniProtKB:Q9P1T7};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAG54082.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP   STAGE.
RC   TISSUE=Oocyte {ECO:0000269|PubMed:11238923};
RX   PubMed=11238923; DOI=10.1128/mcb.21.5.1866-1873.2001;
RA   Snider L., Thirlwell H., Miller J.R., Moon R.T., Groudine M.,
RA   Tapscott S.J.;
RT   "Inhibition of Tcf3 binding by I-mfa domain proteins.";
RL   Mol. Cell. Biol. 21:1866-1873(2001).
RN   [2] {ECO:0000305}
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=15923623; DOI=10.1128/mcb.25.12.5061-5072.2005;
RA   Snider L., Tapscott S.J.;
RT   "XIC is required for Siamois activity and dorsoanterior development.";
RL   Mol. Cell. Biol. 25:5061-5072(2005).
CC   -!- FUNCTION: May act as a transcriptional activator or repressor. Inhibits
CC       the transcriptional activation of Zic family proteins. Retains nuclear
CC       Zic proteins in the cytoplasm (By similarity). Required for
CC       dorsoanterior development. Necessary for siamois to activate downstream
CC       target genes, including gsc, during execution of the dorsal organizer
CC       program. Also regulates the transcriptional activity of TCF7L1/TCF3 by
CC       interacting directly with TCF7L1/TCF3 and preventing it from binding
CC       DNA. {ECO:0000250, ECO:0000269|PubMed:11238923,
CC       ECO:0000269|PubMed:15923623}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9P1T7}. Nucleus
CC       {ECO:0000250|UniProtKB:Q9P1T7}. Nucleus, nucleolus {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed broadly at a low level in the early
CC       embryo. {ECO:0000269|PubMed:11238923, ECO:0000269|PubMed:15923623}.
CC   -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically, with
CC       expression persisting to at least tadpole stages.
CC       {ECO:0000269|PubMed:11238923}.
CC   -!- DOMAIN: The C2H2-type 3, 4 and 5 zinc finger domains are necessary for
CC       transcription activation. The cysteine-rich C-terminus is involved in
CC       its granular distribution in the cytoplasm (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MDFI family. {ECO:0000255}.
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DR   EMBL; AY017419; AAG54082.1; -; mRNA.
DR   RefSeq; NP_001082078.1; NM_001088609.1.
DR   AlphaFoldDB; Q98SK0; -.
DR   BioGRID; 99548; 1.
DR   GeneID; 398213; -.
DR   KEGG; xla:398213; -.
DR   CTD; 398213; -.
DR   Xenbase; XB-GENE-866365; mdfic.S.
DR   OrthoDB; 1487193at2759; -.
DR   Proteomes; UP000186698; Chromosome 3S.
DR   Bgee; 398213; Expressed in egg cell and 19 other tissues.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0140297; F:DNA-binding transcription factor binding; IPI:UniProtKB.
DR   GO; GO:0009950; P:dorsal/ventral axis specification; IMP:UniProtKB.
DR   GO; GO:0043392; P:negative regulation of DNA binding; IDA:UniProtKB.
DR   GO; GO:0042308; P:negative regulation of protein import into nucleus; ISS:UniProtKB.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0030178; P:negative regulation of Wnt signaling pathway; IMP:UniProtKB.
DR   InterPro; IPR026134; MDFI/MDFIC.
DR   PANTHER; PTHR15304; PTHR15304; 1.
DR   Pfam; PF15316; MDFI; 1.
PE   2: Evidence at transcript level;
KW   Activator; Cytoplasm; Developmental protein; Nucleus; Reference proteome;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..246
FT                   /note="MyoD family inhibitor domain-containing protein"
FT                   /id="PRO_0000280224"
FT   DOMAIN          74..246
FT                   /note="MDFI"
FT   REGION          1..93
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          134..164
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        29..44
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        63..92
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        134..160
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   246 AA;  26766 MW;  377B9E79A70F765A CRC64;
     MSQEREPFSP RPEGPEQVTS TEESLLVPLP HGKCEKENMD LEDTTLYRNK SIQTDIEDRS
     ILEDNSNSQP IKAQPQRLPQ PNTSALEQSE EETGKIQNGH VGLSNINGIH NGVKHVPADH
     RNISAPVSQK MHRKIQSSLS VSSDGSKKSK ESSAYSQKPS ASPEDGCVHC ILTCLFCEFL
     TLCNIVVGQA SCGICTSEAC CCCCTEEMGD DCNCPCDMDC GIMDACCESS DCLEICMECC
     GICFPS