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MDHG1_BRANA
ID   MDHG1_BRANA             Reviewed;         358 AA.
AC   Q43743; Q9T0L8;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Malate dehydrogenase 1, glyoxysomal;
DE            EC=1.1.1.37;
DE   Flags: Precursor;
GN   Name=MDH1;
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. Andor; TISSUE=Leaf;
RA   Witt U., Rehberg S., Abel W.O.;
RT   "A full-length cDNA coding for glyoxysomal malate dehydrogenase from
RT   Brassica napus L.";
RL   (er) Plant Gene Register PGR95-124(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RA   Imhoff U., Voetz M., Wingender R., Schnabl H., Wolf N.;
RT   "Two genes encoding microbody malate dehydrogenase from Brassica napus.";
RL   Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate;
CC         Xref=Rhea:RHEA:21432, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.37;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10004};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Glyoxysome.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family.
CC       {ECO:0000305}.
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DR   EMBL; X92512; CAA63268.1; -; mRNA.
DR   EMBL; AJ242712; CAB43994.1; -; Genomic_DNA.
DR   PIR; T08015; T08015.
DR   AlphaFoldDB; Q43743; -.
DR   SMR; Q43743; -.
DR   PRIDE; Q43743; -.
DR   GO; GO:0009514; C:glyoxysome; IEA:UniProtKB-SubCell.
DR   GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   CDD; cd01337; MDH_glyoxysomal_mitochondrial; 1.
DR   Gene3D; 3.90.110.10; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR022383; Lactate/malate_DH_C.
DR   InterPro; IPR001236; Lactate/malate_DH_N.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR001252; Malate_DH_AS.
DR   InterPro; IPR010097; Malate_DH_type1.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF56327; SSF56327; 1.
DR   TIGRFAMs; TIGR01772; MDH_euk_gproteo; 1.
DR   PROSITE; PS00068; MDH; 1.
PE   2: Evidence at transcript level;
KW   Glyoxylate bypass; Glyoxysome; NAD; Oxidoreductase; Peroxisome;
KW   Transit peptide; Tricarboxylic acid cycle.
FT   TRANSIT         1..38
FT                   /note="Glyoxysome"
FT                   /evidence="ECO:0000255"
FT   CHAIN           39..358
FT                   /note="Malate dehydrogenase 1, glyoxysomal"
FT                   /id="PRO_0000018636"
FT   ACT_SITE        222
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         53..59
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         79
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         126
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT   BINDING         132
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT   BINDING         139
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         162..164
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         164
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT   BINDING         198
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT   BINDING         273
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        1..8
FT                   /note="MEFRGDAY -> MPH (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        68
FT                   /note="M -> V (in Ref. 1; CAA63268)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        72
FT                   /note="V -> G (in Ref. 1; CAA63268)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        151..152
FT                   /note="AK -> GG (in Ref. 1; CAA63268)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        231
FT                   /note="L -> S (in Ref. 1; CAA63268)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        235
FT                   /note="K -> T (in Ref. 1; CAA63268)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        251
FT                   /note="R -> K (in Ref. 1; CAA63268)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        254
FT                   /note="N -> H (in Ref. 1; CAA63268)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        258
FT                   /note="E -> D (in Ref. 1; CAA63268)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        263
FT                   /note="K -> H (in Ref. 1; CAA63268)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        266
FT                   /note="A -> V (in Ref. 1; CAA63268)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        269..279
FT                   /note="ATLSMAYAAAK -> SPLPIILAAP (in Ref. 1; CAA63268)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        306..311
FT                   /note="ELAFFA -> DYFLC (in Ref. 1; CAA63268)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   358 AA;  37732 MW;  DC94CBC76ACEEE4A CRC64;
     MEFRGDAYKR IAMISAHLQP SFTPQMEAKN SVMGLESCRA KGGNPGFKVA ILGAAGGIGQ
     SLSLLMKMNP LVSLLHLYDV VNAPGVTADV SHMDTGAVVR GFLGAKQLED ALTGMDLVII
     PAGVPRKPGM TRDDLFKINA GIVRTLCEGV AKCCPNAIVN LISNPVNSTV AIAAEVFKKA
     GTYDPKKLLG VTTLDVARAN TFVAEVLGLD PREVDVPVVG GHAGVTILPL LSQVKPPSSF
     TPSEIEYLTN RIQNGGTEVV EAKAGAGSAT LSMAYAAAKF ADACLRGLRG DANVIECSFV
     ASQVTELAFF ATKVRLGRTG AEEVFQLGPL NEYERVGLEK AKEELAGSIQ KGVDFIRK
 
 
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