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MDHM_BOVIN
ID   MDHM_BOVIN              Reviewed;         338 AA.
AC   Q32LG3;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Malate dehydrogenase, mitochondrial;
DE            EC=1.1.1.37;
DE   Flags: Precursor;
GN   Name=MDH2;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   ACETYLATION AT LYS-185; LYS-307; LYS-314 AND LYS-328, MALONYLATION AT
RP   LYS-239 AND LYS-329, AND SUCCINYLATION AT LYS-239; LYS-301 AND LYS-328.
RX   PubMed=22076378; DOI=10.1126/science.1207861;
RA   Du J., Zhou Y., Su X., Yu J.J., Khan S., Jiang H., Kim J., Woo J.,
RA   Kim J.H., Choi B.H., He B., Chen W., Zhang S., Cerione R.A., Auwerx J.,
RA   Hao Q., Lin H.;
RT   "Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase.";
RL   Science 334:806-809(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate;
CC         Xref=Rhea:RHEA:21432, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.37;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10004};
CC   -!- ACTIVITY REGULATION: Enzyme activity is enhanced by acetylation.
CC       {ECO:0000250|UniProtKB:P40926}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P00346}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix
CC       {ECO:0000250|UniProtKB:P04636}.
CC   -!- PTM: Acetylation is enhanced after treatment either with trichostin A
CC       (TCA) or with nicotinamide (NAM) with the appearance of tri- and
CC       tetraacetylations. Glucose also increases acetylation.
CC       {ECO:0000250|UniProtKB:P40926}.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family.
CC       {ECO:0000305}.
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DR   EMBL; BC109597; AAI09598.1; -; mRNA.
DR   RefSeq; XP_005225065.1; XM_005225008.2.
DR   AlphaFoldDB; Q32LG3; -.
DR   SMR; Q32LG3; -.
DR   IntAct; Q32LG3; 1.
DR   STRING; 9913.ENSBTAP00000012454; -.
DR   iPTMnet; Q32LG3; -.
DR   PaxDb; Q32LG3; -.
DR   PeptideAtlas; Q32LG3; -.
DR   PRIDE; Q32LG3; -.
DR   Ensembl; ENSBTAT00000012454; ENSBTAP00000012454; ENSBTAG00000009462.
DR   GeneID; 281306; -.
DR   CTD; 4191; -.
DR   VEuPathDB; HostDB:ENSBTAG00000009462; -.
DR   VGNC; VGNC:106817; MDH2.
DR   eggNOG; KOG1494; Eukaryota.
DR   GeneTree; ENSGT00390000016686; -.
DR   InParanoid; Q32LG3; -.
DR   OMA; MGWTSQA; -.
DR   OrthoDB; 976445at2759; -.
DR   SABIO-RK; Q32LG3; -.
DR   Proteomes; UP000009136; Chromosome 25.
DR   Bgee; ENSBTAG00000009462; Expressed in cardiac ventricle and 104 other tissues.
DR   ExpressionAtlas; Q32LG3; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0016020; C:membrane; IEA:Ensembl.
DR   GO; GO:0005759; C:mitochondrial matrix; ISS:UniProtKB.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0030060; F:L-malate dehydrogenase activity; ISS:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0009060; P:aerobic respiration; ISS:UniProtKB.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:Ensembl.
DR   GO; GO:0006108; P:malate metabolic process; IEA:Ensembl.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IBA:GO_Central.
DR   CDD; cd01337; MDH_glyoxysomal_mitochondrial; 1.
DR   Gene3D; 3.90.110.10; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR022383; Lactate/malate_DH_C.
DR   InterPro; IPR001236; Lactate/malate_DH_N.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR001252; Malate_DH_AS.
DR   InterPro; IPR010097; Malate_DH_type1.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF56327; SSF56327; 1.
DR   TIGRFAMs; TIGR01772; MDH_euk_gproteo; 1.
DR   PROSITE; PS00068; MDH; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Glycoprotein; Mitochondrion; NAD; Oxidoreductase;
KW   Phosphoprotein; Reference proteome; Transit peptide;
KW   Tricarboxylic acid cycle.
FT   TRANSIT         1..24
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000250|UniProtKB:P00346"
FT   CHAIN           25..338
FT                   /note="Malate dehydrogenase, mitochondrial"
FT                   /id="PRO_0000260267"
FT   ACT_SITE        200
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:P00346"
FT   BINDING         31..37
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   BINDING         57
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   BINDING         104
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT   BINDING         110
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT   BINDING         117
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   BINDING         140..142
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   BINDING         142
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT   BINDING         176
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT   BINDING         251
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   MOD_RES         78
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         78
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         91
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         91
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         165
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   MOD_RES         185
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000269|PubMed:22076378"
FT   MOD_RES         185
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         203
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         215
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         215
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         239
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         239
FT                   /note="N6-malonyllysine; alternate"
FT                   /evidence="ECO:0000269|PubMed:22076378"
FT   MOD_RES         239
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000269|PubMed:22076378"
FT   MOD_RES         246
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   MOD_RES         269
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         296
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         296
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         301
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   MOD_RES         301
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000269|PubMed:22076378"
FT   MOD_RES         307
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000269|PubMed:22076378"
FT   MOD_RES         307
FT                   /note="N6-malonyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   MOD_RES         307
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         314
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000269|PubMed:22076378"
FT   MOD_RES         314
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         324
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         324
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         326
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   MOD_RES         328
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000269|PubMed:22076378"
FT   MOD_RES         328
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000269|PubMed:22076378"
FT   MOD_RES         329
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   MOD_RES         329
FT                   /note="N6-malonyllysine; alternate"
FT                   /evidence="ECO:0000269|PubMed:22076378"
FT   MOD_RES         335
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   MOD_RES         335
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   CARBOHYD        33
FT                   /note="O-linked (GlcNAc) serine"
FT                   /evidence="ECO:0000250|UniProtKB:P04636"
SQ   SEQUENCE   338 AA;  35668 MW;  575B305752E11569 CRC64;
     MLSALARPAG AALRRSFSTS AQNNAKVAVL GASGGIGQPL SLLLKNSPLV SRLTLYDIAH
     TPGVAADLSH IETRATVKGY LGPEQLPDCL KGCDVVVIPA GVPRKPGMTR DDLFNTNATI
     VATLTAACAQ HCPEAMICII SNPVNSTIPI TAEVFKKHGV YNPNKIFGVT TLDIVRANAF
     VAELKDLDPA RVNVPVIGGH AGKTIIPLIS QCTPKVEFPQ DQLTTLTGRI QEAGTEVVKA
     KAGAGSATLS MAYAGARFVF SLVDAMNGKE GVVECSFVKS QETDCPYFST PLLLGKKGIE
     KNLGIGKVSP FEEKMIAEAI PELKASIKKG EEFVKNMK
 
 
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