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MDHM_RAT
ID   MDHM_RAT                Reviewed;         338 AA.
AC   P04636; Q6GSM4;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 185.
DE   RecName: Full=Malate dehydrogenase, mitochondrial;
DE            EC=1.1.1.37;
DE   Flags: Precursor;
GN   Name=Mdh2; Synonyms=Mor1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=3755817; DOI=10.1093/nar/14.15.6053;
RA   Grant P.M., Tellam J., May V.L., Strauss A.W.;
RT   "Isolation and nucleotide sequence of a cDNA clone encoding rat
RT   mitochondrial malate dehydrogenase.";
RL   Nucleic Acids Res. 14:6053-6066(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Pituitary anterior lobe;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   PROTEIN SEQUENCE OF 25-338.
RX   PubMed=3828294; DOI=10.1021/bi00375a019;
RA   Grant P.M., Roderick S.L., Grant G.A., Banaszak L.J., Strauss A.W.;
RT   "Comparison of the precursor and mature forms of rat heart mitochondrial
RT   malate dehydrogenase.";
RL   Biochemistry 26:128-134(1987).
RN   [4]
RP   PROTEIN SEQUENCE OF 27-52; 53-74; 166-185; 192-203; 216-239; 242-296 AND
RP   308-324, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=Sprague-Dawley; TISSUE=Brain, Hippocampus, and Spinal cord;
RA   Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.;
RL   Submitted (JUL-2007) to UniProtKB.
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND TISSUE SPECIFICITY.
RX   PubMed=19423663; DOI=10.1530/rep-09-0052;
RA   Khan S.A., Suryawanshi A.R., Ranpura S.A., Jadhav S.V., Khole V.V.;
RT   "Identification of novel immunodominant epididymal sperm proteins using
RT   combinatorial approach.";
RL   Reproduction 138:81-93(2009).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-246, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
RN   [7]
RP   SUBCELLULAR LOCATION, AND GLYCOSYLATION AT SER-33.
RX   PubMed=24098488; DOI=10.1371/journal.pone.0076399;
RA   Cao W., Cao J., Huang J., Yao J., Yan G., Xu H., Yang P.;
RT   "Discovery and confirmation of O-GlcNAcylated proteins in rat liver
RT   mitochondria by combination of mass spectrometry and immunological
RT   methods.";
RL   PLoS ONE 8:E76399-E76399(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate;
CC         Xref=Rhea:RHEA:21432, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.37;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10004};
CC   -!- ACTIVITY REGULATION: Enzyme activity is enhanced by acetylation.
CC       {ECO:0000250|UniProtKB:P40926}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P00346}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix
CC       {ECO:0000269|PubMed:24098488}.
CC   -!- TISSUE SPECIFICITY: Expressed in flagella of epididymal sperm.
CC       {ECO:0000269|PubMed:19423663}.
CC   -!- PTM: Acetylation is enhanced after treatment either with trichostin A
CC       (TCA) or with nicotinamide (NAM) with the appearance of tri- and
CC       tetraacetylations. Glucose also increases acetylation.
CC       {ECO:0000250|UniProtKB:P40926}.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family.
CC       {ECO:0000305}.
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DR   EMBL; X04240; CAA27812.1; -; mRNA.
DR   EMBL; BC063165; AAH63165.1; -; mRNA.
DR   PIR; A25509; DERTMM.
DR   RefSeq; NP_112413.2; NM_031151.2.
DR   AlphaFoldDB; P04636; -.
DR   SMR; P04636; -.
DR   BioGRID; 249687; 3.
DR   IntAct; P04636; 3.
DR   MINT; P04636; -.
DR   STRING; 10116.ENSRNOP00000001958; -.
DR   ChEMBL; CHEMBL2176835; -.
DR   CarbonylDB; P04636; -.
DR   GlyGen; P04636; 1 site.
DR   iPTMnet; P04636; -.
DR   PhosphoSitePlus; P04636; -.
DR   SwissPalm; P04636; -.
DR   World-2DPAGE; 0004:P04636; -.
DR   jPOST; P04636; -.
DR   PaxDb; P04636; -.
DR   PRIDE; P04636; -.
DR   Ensembl; ENSRNOT00000001958; ENSRNOP00000001958; ENSRNOG00000001440.
DR   GeneID; 81829; -.
DR   KEGG; rno:81829; -.
DR   UCSC; RGD:619719; rat.
DR   CTD; 4191; -.
DR   RGD; 619719; Mdh2.
DR   eggNOG; KOG1494; Eukaryota.
DR   GeneTree; ENSGT00390000016686; -.
DR   HOGENOM; CLU_047181_0_1_1; -.
DR   InParanoid; P04636; -.
DR   OMA; MGWTSQA; -.
DR   OrthoDB; 976445at2759; -.
DR   PhylomeDB; P04636; -.
DR   TreeFam; TF300834; -.
DR   Reactome; R-RNO-70263; Gluconeogenesis.
DR   Reactome; R-RNO-71403; Citric acid cycle (TCA cycle).
DR   PRO; PR:P04636; -.
DR   Proteomes; UP000002494; Chromosome 12.
DR   Bgee; ENSRNOG00000001440; Expressed in duodenum and 20 other tissues.
DR   Genevisible; P04636; RN.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0016020; C:membrane; ISO:RGD.
DR   GO; GO:0005759; C:mitochondrial matrix; IDA:RGD.
DR   GO; GO:0005739; C:mitochondrion; IDA:RGD.
DR   GO; GO:0030060; F:L-malate dehydrogenase activity; IDA:RGD.
DR   GO; GO:0046554; F:malate dehydrogenase (NADP+) activity; IDA:RGD.
DR   GO; GO:0016615; F:malate dehydrogenase activity; IDA:RGD.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0043621; F:protein self-association; IDA:RGD.
DR   GO; GO:0009060; P:aerobic respiration; ISO:RGD.
DR   GO; GO:0006094; P:gluconeogenesis; ISO:RGD.
DR   GO; GO:0006108; P:malate metabolic process; IDA:RGD.
DR   GO; GO:0006734; P:NADH metabolic process; IDA:RGD.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IDA:RGD.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IDA:RGD.
DR   CDD; cd01337; MDH_glyoxysomal_mitochondrial; 1.
DR   Gene3D; 3.90.110.10; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR022383; Lactate/malate_DH_C.
DR   InterPro; IPR001236; Lactate/malate_DH_N.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR001252; Malate_DH_AS.
DR   InterPro; IPR010097; Malate_DH_type1.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF56327; SSF56327; 1.
DR   TIGRFAMs; TIGR01772; MDH_euk_gproteo; 1.
DR   PROSITE; PS00068; MDH; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Direct protein sequencing; Glycoprotein; Mitochondrion; NAD;
KW   Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide;
KW   Tricarboxylic acid cycle.
FT   TRANSIT         1..24
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000269|PubMed:3828294"
FT   CHAIN           25..338
FT                   /note="Malate dehydrogenase, mitochondrial"
FT                   /id="PRO_0000018631"
FT   ACT_SITE        200
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:P00346"
FT   BINDING         31..37
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   BINDING         57
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   BINDING         104
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT   BINDING         110
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT   BINDING         117
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   BINDING         140..142
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   BINDING         142
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT   BINDING         176
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT   BINDING         251
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   MOD_RES         78
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         78
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         91
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         91
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         165
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   MOD_RES         185
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q32LG3"
FT   MOD_RES         185
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         203
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         215
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         215
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         239
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         239
FT                   /note="N6-malonyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q32LG3"
FT   MOD_RES         239
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q32LG3"
FT   MOD_RES         246
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         269
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         296
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         296
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         301
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   MOD_RES         301
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q32LG3"
FT   MOD_RES         307
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q32LG3"
FT   MOD_RES         307
FT                   /note="N6-malonyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   MOD_RES         307
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         314
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q32LG3"
FT   MOD_RES         314
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         324
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         324
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   MOD_RES         326
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   MOD_RES         328
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q32LG3"
FT   MOD_RES         328
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q32LG3"
FT   MOD_RES         329
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   MOD_RES         329
FT                   /note="N6-malonyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q32LG3"
FT   MOD_RES         335
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P40926"
FT   MOD_RES         335
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P08249"
FT   CARBOHYD        33
FT                   /note="O-linked (GlcNAc) serine"
FT                   /evidence="ECO:0000269|PubMed:24098488"
FT   CONFLICT        229
FT                   /note="R -> K (in Ref. 1; CAA27812)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   338 AA;  35684 MW;  13849CCFB78C87E7 CRC64;
     MLSALARPVG AALRRSFSTS AQNNAKVAVL GASGGIGQPL SLLLKNSPLV SRLTLYDIAH
     TPGVAADLSH IETRANVKGY LGPEQLPDCL KGCDVVVIPA GVPRKPGMTR DDLFNTNATI
     VATLTAACAQ HCPEAMICII SNPVNSTIPI TAEVFKKHGV YNPNKIFGVT TLDIVRANTF
     VAELKGLDPA RVNVPVIGGH AGKTIIPLIS QCTPKVDFPQ DQLATLTGRI QEAGTEVVKA
     KAGAGSATLS MAYAGARFVF SLVDAMNGKE GVIECSFVQS KETECTYFST PLLLGKKGLE
     KNLGIGKITP FEEKMIAEAI PELKASIKKG EDFVKNMK
 
 
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