MDHM_SCHMA
ID MDHM_SCHMA Reviewed; 142 AA.
AC P37227;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Malate dehydrogenase, mitochondrial;
DE EC=1.1.1.37;
DE Flags: Fragment;
OS Schistosoma mansoni (Blood fluke).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes; Trematoda;
OC Digenea; Strigeidida; Schistosomatoidea; Schistosomatidae; Schistosoma.
OX NCBI_TaxID=6183;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8396206; DOI=10.1016/0166-6851(93)90032-s;
RA Skelly P.J., Stein L.D., Shoemaker C.B.;
RT "Expression of Schistosoma mansoni genes involved in anaerobic and
RT oxidative glucose metabolism during the cercaria to adult transformation.";
RL Mol. Biochem. Parasitol. 60:93-104(1993).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate;
CC Xref=Rhea:RHEA:21432, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.37;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10004};
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC -!- DEVELOPMENTAL STAGE: Expressed in relatively high level in adults as
CC compared to larval worms.
CC -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family.
CC {ECO:0000305}.
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DR EMBL; L10108; AAA29901.1; -; Genomic_DNA.
DR AlphaFoldDB; P37227; -.
DR SMR; P37227; -.
DR STRING; 6183.Smp_047370.1; -.
DR eggNOG; KOG1494; Eukaryota.
DR HOGENOM; CLU_047181_0_1_1; -.
DR SABIO-RK; P37227; -.
DR Proteomes; UP000008854; Unassembled WGS sequence.
DR GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR InterPro; IPR001236; Lactate/malate_DH_N.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF00056; Ldh_1_N; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Mitochondrion; NAD; Oxidoreductase; Reference proteome;
KW Tricarboxylic acid cycle.
FT CHAIN <1..>142
FT /note="Malate dehydrogenase, mitochondrial"
FT /id="PRO_0000113340"
FT BINDING 1..6
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 26
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 73
FT /ligand="substrate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT BINDING 79
FT /ligand="substrate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT BINDING 86
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 109..111
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 111
FT /ligand="substrate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT NON_TER 1
FT NON_TER 142
SQ SEQUENCE 142 AA; 14956 MW; C06BC6726566AE72 CRC64;
ASGGIGEPLS LLLKQSPLIS QLALYDIAHV KGVAADLSHI ETQAHVTAHL GPGELAECLT
GANVVIIPAG LPRKPGMTRD DLFNTNASIV AELIDSCAKN CPKAMICIIT NPVNSTVPIA
AEILKRHNVY DPKRLFGVTT LD
