MDH_HALVD
ID MDH_HALVD Reviewed; 304 AA.
AC Q9P9L2; D4GYB6; Q0MSE3;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 2.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Malate dehydrogenase {ECO:0000250|UniProtKB:O08349};
DE EC=1.1.1.37 {ECO:0000250|UniProtKB:O08349};
GN Name=mdh; OrderedLocusNames=HVO_3007;
OS Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Haloferacaceae; Haloferax.
OX NCBI_TaxID=309800;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Mevarech M.;
RT "The gene coding for Haloferax volcanii malate dehydrogenase.";
RL Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RA Tetsch L., Galinski E.A.;
RT "Expression of halophilic malate dehydrogenase of Haloferax volcanii.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT "The complete genome sequence of Haloferax volcanii DS2, a model
RT archaeon.";
RL PLoS ONE 5:E9605-E9605(2010).
CC -!- FUNCTION: Catalyzes the reversible oxidation of malate to oxaloacetate.
CC {ECO:0000250|UniProtKB:O08349}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate;
CC Xref=Rhea:RHEA:21432, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.37;
CC Evidence={ECO:0000250|UniProtKB:O08349};
CC -!- SIMILARITY: Belongs to the LDH/MDH superfamily. {ECO:0000305}.
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DR EMBL; AF236112; AAF43044.1; -; Genomic_DNA.
DR EMBL; DQ851095; ABH10498.1; -; Genomic_DNA.
DR EMBL; CP001956; ADE04975.1; -; Genomic_DNA.
DR RefSeq; WP_004044925.1; NZ_AOHU01000104.1.
DR PDB; 4BGU; X-ray; 1.49 A; A/B/C/D=2-304.
DR PDBsum; 4BGU; -.
DR AlphaFoldDB; Q9P9L2; -.
DR SMR; Q9P9L2; -.
DR STRING; 309800.C498_18200; -.
DR EnsemblBacteria; ADE04975; ADE04975; HVO_3007.
DR GeneID; 8925993; -.
DR KEGG; hvo:HVO_3007; -.
DR eggNOG; arCOG00246; Archaea.
DR HOGENOM; CLU_045401_1_1_2; -.
DR OMA; CYIIVLT; -.
DR OrthoDB; 69007at2157; -.
DR Proteomes; UP000008243; Chromosome.
DR GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR Gene3D; 3.90.110.10; -; 1.
DR InterPro; IPR001557; L-lactate/malate_DH.
DR InterPro; IPR022383; Lactate/malate_DH_C.
DR InterPro; IPR001236; Lactate/malate_DH_N.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF02866; Ldh_1_C; 1.
DR Pfam; PF00056; Ldh_1_N; 1.
DR PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR PRINTS; PR00086; LLDHDRGNASE.
DR SUPFAM; SSF51735; SSF51735; 1.
DR SUPFAM; SSF56327; SSF56327; 1.
PE 1: Evidence at protein level;
KW 3D-structure; NAD; Oxidoreductase; Reference proteome;
KW Tricarboxylic acid cycle.
FT CHAIN 1..304
FT /note="Malate dehydrogenase"
FT /id="PRO_0000113483"
FT ACT_SITE 176
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:P61889"
FT BINDING 8..14
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:O08349"
FT BINDING 34
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:O08349"
FT BINDING 83
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P61889"
FT BINDING 89
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P61889"
FT BINDING 96
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:O08349"
FT BINDING 119..121
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:O08349"
FT BINDING 121
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P61889"
FT BINDING 152
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P61889"
FT CONFLICT 17
FT /note="A -> G (in Ref. 1; AAF43044)"
FT /evidence="ECO:0000305"
FT CONFLICT 285
FT /note="Missing (in Ref. 1; AAF43044)"
FT /evidence="ECO:0000305"
FT CONFLICT 290
FT /note="D -> N (in Ref. 1; AAF43044)"
FT /evidence="ECO:0000305"
FT CONFLICT 297
FT /note="R -> L (in Ref. 1; AAF43044)"
FT /evidence="ECO:0000305"
FT STRAND 3..8
FT /evidence="ECO:0007829|PDB:4BGU"
FT HELIX 12..24
FT /evidence="ECO:0007829|PDB:4BGU"
FT STRAND 28..33
FT /evidence="ECO:0007829|PDB:4BGU"
FT HELIX 36..38
FT /evidence="ECO:0007829|PDB:4BGU"
FT HELIX 39..53
FT /evidence="ECO:0007829|PDB:4BGU"
FT TURN 54..56
FT /evidence="ECO:0007829|PDB:4BGU"
FT STRAND 60..63
FT /evidence="ECO:0007829|PDB:4BGU"
FT HELIX 66..69
FT /evidence="ECO:0007829|PDB:4BGU"
FT STRAND 73..77
FT /evidence="ECO:0007829|PDB:4BGU"
FT HELIX 89..108
FT /evidence="ECO:0007829|PDB:4BGU"
FT STRAND 115..118
FT /evidence="ECO:0007829|PDB:4BGU"
FT HELIX 123..132
FT /evidence="ECO:0007829|PDB:4BGU"
FT STRAND 134..136
FT /evidence="ECO:0007829|PDB:4BGU"
FT HELIX 138..140
FT /evidence="ECO:0007829|PDB:4BGU"
FT STRAND 141..143
FT /evidence="ECO:0007829|PDB:4BGU"
FT HELIX 146..161
FT /evidence="ECO:0007829|PDB:4BGU"
FT HELIX 165..167
FT /evidence="ECO:0007829|PDB:4BGU"
FT STRAND 172..174
FT /evidence="ECO:0007829|PDB:4BGU"
FT STRAND 180..182
FT /evidence="ECO:0007829|PDB:4BGU"
FT HELIX 184..186
FT /evidence="ECO:0007829|PDB:4BGU"
FT HELIX 198..216
FT /evidence="ECO:0007829|PDB:4BGU"
FT TURN 217..219
FT /evidence="ECO:0007829|PDB:4BGU"
FT HELIX 224..238
FT /evidence="ECO:0007829|PDB:4BGU"
FT STRAND 244..253
FT /evidence="ECO:0007829|PDB:4BGU"
FT HELIX 254..256
FT /evidence="ECO:0007829|PDB:4BGU"
FT STRAND 258..269
FT /evidence="ECO:0007829|PDB:4BGU"
FT STRAND 272..276
FT /evidence="ECO:0007829|PDB:4BGU"
FT HELIX 283..303
FT /evidence="ECO:0007829|PDB:4BGU"
SQ SEQUENCE 304 AA; 32654 MW; 7AD72EDC9C137268 CRC64;
MTKVSVIGAA GTVGAAAGYN LALRDVCDEL VFVDIPKMED KTVGQAADTN HGIAYDSNTV
VTQGGYEDTA GSDVVVITAG IPRQPGQTRI DLAGDNAPIM DDIGSSLAEY NDDFVSITTS
NPVDLLNRHL YETGDRDRHK VIGFGGRLDS ARFRYVLSQR FDVPVKNVDA TILGEHGDAQ
VPVFSKVRVD GNDPAFSADE KEEILGDLQE SAMDVIERKG ATQWGPATGV AHMVEAVLHD
TGEVLPGSLV LDGEFGYEDT AFGVPVKLGS NGIEEVVEWD LDDYEADLMD DAAEKLRDQY
DEIA
