ID   MDH_HELGE               Reviewed;          30 AA.
AC   P80037;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Malate dehydrogenase {ECO:0000250|UniProtKB:Q25QU7};
DE            EC=1.1.1.37 {ECO:0000250|UniProtKB:Q25QU7};
DE   Flags: Fragment;
GN   Name=mdh;
OS   Heliomicrobium gestii (Heliobacterium gestii).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Heliobacteriaceae;
OC   Heliomicrobium.
OX   NCBI_TaxID=2699;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=1735722; DOI=10.1128/jb.174.4.1307-1313.1992;
RA   Charnock C.B., Refseth U.H., Strevaag R.;
RT   "Malate dehydrogenase from Chlorobium vibrioforme, Chlorobium tepidum, and
RT   Heliobacterium gestii: purification, characterization, and investigation of
RT   dinucleotide binding by dehydrogenases by use of empirical methods of
RT   protein sequence analysis.";
RL   J. Bacteriol. 174:1307-1313(1992).
CC   -!- FUNCTION: Catalyzes the reversible oxidation of malate to oxaloacetate.
CC       {ECO:0000250|UniProtKB:Q25QU7}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate;
CC         Xref=Rhea:RHEA:21432, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.37;
CC         Evidence={ECO:0000250|UniProtKB:Q25QU7};
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 3 family.
CC       {ECO:0000305}.
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DR   GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; NAD; Oxidoreductase; Tricarboxylic acid cycle.
FT   CHAIN           1..>30
FT                   /note="Malate dehydrogenase"
FT                   /id="PRO_0000113455"
FT   BINDING         9..14
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P80040"
FT   NON_TER         30
SQ   SEQUENCE   30 AA;  2910 MW;  958743720AACF319 CRC64;
     MTKKITIIGA GNVGATAAXX AASKDLGDIV