MDH_KLEPR
ID MDH_KLEPR Reviewed; 114 AA.
AC P0C1G5; P80535;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2006, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=Malate dehydrogenase;
DE EC=1.1.1.37;
DE Flags: Fragment;
GN Name=mdh;
OS Klebsiella pneumoniae subsp. rhinoscleromatis.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=39831;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 13884 / JCM 1664 / LMG 3184 / NCTC 5046 / R-70;
RX PubMed=15215087; DOI=10.1128/aac.48.7.2400-2408.2004;
RA Haeggman S., Loefdahl S., Paauw A., Verhoef J., Brisse S.;
RT "Diversity and evolution of the class A chromosomal beta-lactamase gene in
RT Klebsiella pneumoniae.";
RL Antimicrob. Agents Chemother. 48:2400-2408(2004).
CC -!- FUNCTION: Catalyzes the reversible oxidation of malate to oxaloacetate.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate;
CC Xref=Rhea:RHEA:21432, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.37;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10004};
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family.
CC {ECO:0000305}.
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DR EMBL; AJ635391; CAG25802.1; -; Genomic_DNA.
DR AlphaFoldDB; P0C1G5; -.
DR SMR; P0C1G5; -.
DR GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR InterPro; IPR001236; Lactate/malate_DH_N.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF00056; Ldh_1_N; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 3: Inferred from homology;
KW NAD; Oxidoreductase; Tricarboxylic acid cycle.
FT CHAIN <1..>114
FT /note="Malate dehydrogenase"
FT /id="PRO_0000240201"
FT BINDING 4
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 51
FT /ligand="substrate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT BINDING 57
FT /ligand="substrate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT BINDING 64
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 87..89
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 89
FT /ligand="substrate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT NON_TER 1
FT NON_TER 114
SQ SEQUENCE 114 AA; 11913 MW; D63AEB7FD35062AE CRC64;
SLYDIAPVTP GVAVDLSHIP TDVKIKGFSG EDATPALEGA DVVLISAGVA RKPGMDRSDL
FNVNAGIVKN LVQQIAKTRP QACIGIITNP VNTTVAIAAE VLKKAGVYDK NKLF
