MDH_PECCC
ID MDH_PECCC Reviewed; 154 AA.
AC P61897;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2004, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Malate dehydrogenase;
DE EC=1.1.1.37;
DE Flags: Fragment;
GN Name=mdh;
OS Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp.
OS carotovora).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Pectobacteriaceae; Pectobacterium.
OX NCBI_TaxID=555;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=WPP1, WPP10, WPP11, WPP12, WPP13, WPP14, WPP15, WPP17, WPP18, WPP19,
RC WPP2, WPP20, WPP21, WPP22, WPP24, WPP25, WPP3, WPP4, WPP5, WPP6, WPP7,
RC WPP8, and WPP9;
RX PubMed=15128563; DOI=10.1128/aem.70.5.3013-3023.2004;
RA Yap M.-N., Barak J.D., Charkowski A.O.;
RT "Genomic diversity of Erwinia carotovora subsp. carotovora and its
RT correlation with virulence.";
RL Appl. Environ. Microbiol. 70:3013-3023(2004).
CC -!- FUNCTION: Catalyzes the reversible oxidation of malate to oxaloacetate.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate;
CC Xref=Rhea:RHEA:21432, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.37;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10004};
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family.
CC {ECO:0000305}.
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DR EMBL; AY428968; AAR11889.1; -; Genomic_DNA.
DR EMBL; AY428969; AAR11890.1; -; Genomic_DNA.
DR EMBL; AY428970; AAR11891.1; -; Genomic_DNA.
DR EMBL; AY428971; AAR11892.1; -; Genomic_DNA.
DR EMBL; AY428972; AAR11893.1; -; Genomic_DNA.
DR EMBL; AY428973; AAR11894.1; -; Genomic_DNA.
DR EMBL; AY428974; AAR11895.1; -; Genomic_DNA.
DR EMBL; AY428975; AAR11896.1; -; Genomic_DNA.
DR EMBL; AY428976; AAR11897.1; -; Genomic_DNA.
DR EMBL; AY428977; AAR11898.1; -; Genomic_DNA.
DR EMBL; AY428978; AAR11899.1; -; Genomic_DNA.
DR EMBL; AY428979; AAR11900.1; -; Genomic_DNA.
DR EMBL; AY428980; AAR11901.1; -; Genomic_DNA.
DR EMBL; AY428981; AAR11902.1; -; Genomic_DNA.
DR EMBL; AY428982; AAR11903.1; -; Genomic_DNA.
DR EMBL; AY428983; AAR11904.1; -; Genomic_DNA.
DR EMBL; AY428984; AAR11905.1; -; Genomic_DNA.
DR EMBL; AY428985; AAR11906.1; -; Genomic_DNA.
DR EMBL; AY428986; AAR11907.1; -; Genomic_DNA.
DR EMBL; AY428987; AAR11908.1; -; Genomic_DNA.
DR EMBL; AY428988; AAR11909.1; -; Genomic_DNA.
DR EMBL; AY428989; AAR11910.1; -; Genomic_DNA.
DR EMBL; AY428990; AAR11911.1; -; Genomic_DNA.
DR AlphaFoldDB; P61897; -.
DR SMR; P61897; -.
DR GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR Gene3D; 3.90.110.10; -; 1.
DR InterPro; IPR022383; Lactate/malate_DH_C.
DR InterPro; IPR001236; Lactate/malate_DH_N.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR001252; Malate_DH_AS.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF02866; Ldh_1_C; 1.
DR Pfam; PF00056; Ldh_1_N; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR SUPFAM; SSF56327; SSF56327; 1.
DR PROSITE; PS00068; MDH; 1.
PE 3: Inferred from homology;
KW NAD; Oxidoreductase; Tricarboxylic acid cycle.
FT CHAIN <1..>154
FT /note="Malate dehydrogenase"
FT /id="PRO_0000113318"
FT ACT_SITE 99
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 3
FT /ligand="substrate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT BINDING 9
FT /ligand="substrate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT BINDING 16
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 39..41
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 41
FT /ligand="substrate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT BINDING 75
FT /ligand="substrate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10004"
FT BINDING 149
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT VARIANT 15..17
FT /note="VNA -> LPF (in strain: WPP3)"
FT VARIANT 23..24
FT /note="LV -> WH (in strain: WPP3)"
FT VARIANT 32
FT /note="P -> R (in strain: WPP3)"
FT VARIANT 49
FT /note="I -> M (in strain: WPP7)"
FT VARIANT 106..107
FT /note="PL -> LC (in strain: WPP25)"
FT VARIANT 111
FT /note="V -> D (in strain: WPP25)"
FT VARIANT 117
FT /note="S -> N (in strain: WPP3 and WPP8)"
FT VARIANT 133..134
FT /note="TE -> AQ (in strain: WPP3)"
FT VARIANT 137..140
FT /note="EAKA -> KPKQ (in strain: WPP19)"
FT VARIANT 137
FT /note="E -> K (in strain: WPP3)"
FT VARIANT 141
FT /note="G -> R (in strain: WPP25)"
FT VARIANT 142
FT /note="G -> A (in strain: WPP9 and WPP10)"
FT VARIANT 150..154
FT /note="GQVPG -> ARLPA (in strain: WPP25)"
FT VARIANT 152..154
FT /note="VPG -> AAR (in strain: WPP20, WPP22 and WPP7)"
FT VARIANT 152..154
FT /note="VPG -> AGR (in strain: WPP5)"
FT VARIANT 152..153
FT /note="VP -> AA (in strain: WPP12 and WPP21)"
FT VARIANT 152
FT /note="V -> A (in strain: WPP24)"
FT VARIANT 153
FT /note="P -> A (in strain: WPP4)"
FT NON_TER 1
FT NON_TER 154
SQ SEQUENCE 154 AA; 16149 MW; 40417ECADE8C1877 CRC64;
LRRKPGMDRS DLFNVNAGIV RNLVEQIAVT CPKACIGIIT NPVNTTVAIA AEVLKKAGVY
DKNKLFGVTT LDIIRSNTFV AELKGKQPQD INVPVIGGHS GVTILPLLSQ VPGISFSEQE
VADLTKRIQN AGTEVVEAKA GGGSATLSMG QVPG
