位置:首页 > 蛋白库 > MDH_PYRHO
MDH_PYRHO
ID   MDH_PYRHO               Reviewed;         360 AA.
AC   O59028;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Malate dehydrogenase;
DE            EC=1.1.1.37;
GN   Name=mdh; OrderedLocusNames=PH1277;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS   100139 / OT-3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA   Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA   Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA   Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA   Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate;
CC         Xref=Rhea:RHEA:21432, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.37;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the LDH2/MDH2 oxidoreductase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BA000001; BAA30380.1; -; Genomic_DNA.
DR   PIR; B71073; B71073.
DR   RefSeq; WP_010885364.1; NC_000961.1.
DR   PDB; 1V9N; X-ray; 2.10 A; A=1-360.
DR   PDBsum; 1V9N; -.
DR   AlphaFoldDB; O59028; -.
DR   SMR; O59028; -.
DR   STRING; 70601.3257697; -.
DR   PRIDE; O59028; -.
DR   EnsemblBacteria; BAA30380; BAA30380; BAA30380.
DR   GeneID; 1443600; -.
DR   KEGG; pho:PH1277; -.
DR   eggNOG; arCOG04874; Archaea.
DR   OMA; TNTEPAM; -.
DR   OrthoDB; 32318at2157; -.
DR   EvolutionaryTrace; O59028; -.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1530.10; -; 1.
DR   Gene3D; 3.30.1370.60; -; 1.
DR   InterPro; IPR043144; Mal/L-sulf/L-lact_DH-like_ah.
DR   InterPro; IPR043143; Mal/L-sulf/L-lact_DH-like_NADP.
DR   InterPro; IPR036111; Mal/L-sulfo/L-lacto_DH-like_sf.
DR   InterPro; IPR003767; Malate/L-lactate_DH-like.
DR   PANTHER; PTHR11091; PTHR11091; 1.
DR   Pfam; PF02615; Ldh_2; 1.
DR   SUPFAM; SSF89733; SSF89733; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; NAD; Oxidoreductase; Tricarboxylic acid cycle.
FT   CHAIN           1..360
FT                   /note="Malate dehydrogenase"
FT                   /id="PRO_0000083829"
FT   STRAND          11..14
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   HELIX           16..29
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   HELIX           34..49
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   HELIX           53..55
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   HELIX           57..59
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   HELIX           60..68
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   STRAND          71..75
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   STRAND          79..84
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   STRAND          87..91
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   HELIX           97..115
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   STRAND          116..125
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   HELIX           132..139
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   TURN            140..142
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   STRAND          143..149
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   STRAND          170..174
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   STRAND          177..179
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   STRAND          181..185
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   STRAND          187..190
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   TURN            192..194
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   STRAND          215..217
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   TURN            223..227
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   STRAND          236..238
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   HELIX           242..256
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   TURN            257..261
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   HELIX           265..267
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   STRAND          278..285
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   HELIX           287..289
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   HELIX           293..308
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   HELIX           324..336
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   STRAND          338..341
FT                   /evidence="ECO:0007829|PDB:1V9N"
FT   HELIX           342..355
FT                   /evidence="ECO:0007829|PDB:1V9N"
SQ   SEQUENCE   360 AA;  39751 MW;  6E9D8B16ECDE6E6F CRC64;
     MFEKGYVDEN YIRVPKDRLF SFIVRVLTKL GVPEEDAKIV ADNLVMADLR GVESHGVQRL
     KRYVDGIISG GVNLHPKIRV IREGPSYALI DGDEGLGQVV GYRSMKLAIK KAKDTGIGIV
     IARNSNHYGI AGYYALMAAE EGMIGISMTN SRPLVAPTGG IERILGTNPI ALAAPTKDKP
     FLLDMATSVV PIGKLEVYRR KGKDIPEGWA INREGNITTK VEEVFNGGAL LPLGGFGELL
     GGHKGYGLSL MVDILSGILS GGTWSKYVKN TSEKGSNVCH FFMVIDIEHF IPLEEFKEKI
     SQMIEEIKSS RKHPEFERIW IHGEKGFLTM ETRLKLGIPI YRKVLEELNE IAKRVGVEGL
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024