MDH_VIBCH
ID MDH_VIBCH Reviewed; 311 AA.
AC Q9KUT3; Q8GN36; Q8GN37; Q8GN38; Q8GN39; Q99PZ5; Q99Q77; Q99QN5; Q9AG49;
AC Q9AG50; Q9AIU2; Q9R3N1; Q9R3N2; Q9R3N3; Q9S4R7; Q9XCZ8;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Malate dehydrogenase;
DE EC=1.1.1.37;
GN Name=mdh; OrderedLocusNames=VC_0432;
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M535, M536, M548, M549, M550, M551, M553, M554, M555, M556, M557,
RC M558, M559, M560, M561, M562, M563, M645, and M793;
RX PubMed=10024551; DOI=10.1128/iai.67.3.1116-1124.1999;
RA Byun R., Elbourne L.D., Lan R., Reeves P.R.;
RT "Evolutionary relationships of pathogenic clones of Vibrio cholerae by
RT sequence analysis of four housekeeping genes.";
RL Infect. Immun. 67:1116-1124(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-248.
RC STRAIN=151 / Serotype O37, 208 / Serotype O11,
RC ATCC 25870 / Classical Inaba 569B / Serotype O1, CO130 / Serotype O37,
RC V46 / Serotype O141, and V52 / Serotype O37;
RX PubMed=10986258; DOI=10.1128/jb.182.19.5530-5538.2000;
RA Boyd E.F., Heilpern A.J., Waldor M.K.;
RT "Molecular analyses of a putative CTXphi precursor and evidence for
RT independent acquisition of distinct CTXphis by toxigenic Vibrio cholerae.";
RL J. Bacteriol. 182:5530-5538(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 38-201.
RC STRAIN=25872, 329 / Serotype O139, 653/36 / Serotype O139,
RC BO1 / Serotype O139, BO2 / Serotype O139, BO4 / Serotype O139,
RC CO391 / Serotype O139, CO396 / Serotype O139, CO402 / Serotype O139,
RC CO403 / Serotype O139, CO404 / Serotype O139, CO406 / Serotype O139,
RC CO407 / Serotype O139, CO414 / Serotype O139, CO415 / Serotype O139,
RC CO418 / Serotype O139, CO487 / Serotype O1, MDO90 / Serotype O139,
RC MOD084 / Serotype O139, NPO388 / Serotype O139, NPO390 / Serotype O139,
RC NT329 / Serotype O139, NT330 / Serotype O139, NT638 / Serotype O139,
RC NT642 / Serotype O139, NT648 / Serotype O139, SG24 / Serotype O139,
RC SO19 / Serotype O139, SO29 / Serotype O139, SO30 / Serotype O139, and
RC VO6 / Serotype O139;
RX PubMed=11844759; DOI=10.1128/jb.184.5.1304-1313.2002;
RA Farfan M., Minana-Galbis D., Fuste M.C., Loren J.G.;
RT "Allelic diversity and population structure in Vibrio cholerae O139 Bengal
RT based on nucleotide sequence analysis.";
RL J. Bacteriol. 184:1304-1313(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 111-310.
RC STRAIN=GD64313 / Serotype O1, GD9512 / Serotype O139,
RC GD98200 / Serotype O139, and GD98224 / Serotype O1;
RA Yongyu R., Biao K., Shouyi G.;
RT "Housekeeping genes of strains of Vibrio cholerae isolated from Guangdong
RT province.";
RL Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the reversible oxidation of malate to oxaloacetate.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate;
CC Xref=Rhea:RHEA:21432, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.37;
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF93605.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF117859; AAD23488.1; -; Genomic_DNA.
DR EMBL; AF117860; AAD23489.1; -; Genomic_DNA.
DR EMBL; AF117861; AAD23490.1; -; Genomic_DNA.
DR EMBL; AF117862; AAD23491.1; -; Genomic_DNA.
DR EMBL; AF117863; AAD23492.1; -; Genomic_DNA.
DR EMBL; AF117864; AAD23493.1; -; Genomic_DNA.
DR EMBL; AF117865; AAD23494.1; -; Genomic_DNA.
DR EMBL; AF117866; AAD23495.1; -; Genomic_DNA.
DR EMBL; AF117867; AAD23496.1; -; Genomic_DNA.
DR EMBL; AF117868; AAD23497.1; -; Genomic_DNA.
DR EMBL; AF117869; AAD23498.1; -; Genomic_DNA.
DR EMBL; AF117870; AAD23499.1; -; Genomic_DNA.
DR EMBL; AF117871; AAD23500.1; -; Genomic_DNA.
DR EMBL; AF117872; AAD23501.1; -; Genomic_DNA.
DR EMBL; AF117873; AAD23502.1; -; Genomic_DNA.
DR EMBL; AF117874; AAD23503.1; -; Genomic_DNA.
DR EMBL; AF117875; AAD23504.1; -; Genomic_DNA.
DR EMBL; AF117876; AAD23505.1; -; Genomic_DNA.
DR EMBL; AF117877; AAD23506.1; -; Genomic_DNA.
DR EMBL; AE003852; AAF93605.1; ALT_INIT; Genomic_DNA.
DR EMBL; AF238329; AAK15054.1; -; Genomic_DNA.
DR EMBL; AF238330; AAK15055.1; -; Genomic_DNA.
DR EMBL; AF238331; AAK15056.1; -; Genomic_DNA.
DR EMBL; AF238332; AAK15057.1; -; Genomic_DNA.
DR EMBL; AF238333; AAK15058.1; -; Genomic_DNA.
DR EMBL; AF238334; AAK15059.1; -; Genomic_DNA.
DR EMBL; AF343280; AAK30532.1; -; Genomic_DNA.
DR EMBL; AF343281; AAK30533.1; -; Genomic_DNA.
DR EMBL; AF343282; AAK30534.1; -; Genomic_DNA.
DR EMBL; AF343283; AAK30535.1; -; Genomic_DNA.
DR EMBL; AF343284; AAK30536.1; -; Genomic_DNA.
DR EMBL; AF343285; AAK30537.1; -; Genomic_DNA.
DR EMBL; AF343286; AAK30538.1; -; Genomic_DNA.
DR EMBL; AF343287; AAK30539.1; -; Genomic_DNA.
DR EMBL; AF343288; AAK30540.1; -; Genomic_DNA.
DR EMBL; AF343289; AAK30541.1; -; Genomic_DNA.
DR EMBL; AF343290; AAK30542.1; -; Genomic_DNA.
DR EMBL; AF343291; AAK30543.1; -; Genomic_DNA.
DR EMBL; AF343292; AAK30544.1; -; Genomic_DNA.
DR EMBL; AF343293; AAK30545.1; -; Genomic_DNA.
DR EMBL; AF343294; AAK30546.1; -; Genomic_DNA.
DR EMBL; AF343295; AAK30547.1; -; Genomic_DNA.
DR EMBL; AF343296; AAK30548.1; -; Genomic_DNA.
DR EMBL; AF343297; AAK30549.1; -; Genomic_DNA.
DR EMBL; AF343298; AAK30550.1; -; Genomic_DNA.
DR EMBL; AF343299; AAK30551.1; -; Genomic_DNA.
DR EMBL; AF343300; AAK30552.1; -; Genomic_DNA.
DR EMBL; AF343301; AAK30553.1; -; Genomic_DNA.
DR EMBL; AF343302; AAK30554.1; -; Genomic_DNA.
DR EMBL; AF343303; AAK30555.1; -; Genomic_DNA.
DR EMBL; AF343304; AAK30556.1; -; Genomic_DNA.
DR EMBL; AF343305; AAK30557.1; -; Genomic_DNA.
DR EMBL; AF343306; AAK30558.1; -; Genomic_DNA.
DR EMBL; AF343307; AAK30559.1; -; Genomic_DNA.
DR EMBL; AF343308; AAK30560.1; -; Genomic_DNA.
DR EMBL; AF343309; AAK30561.1; -; Genomic_DNA.
DR EMBL; AF343310; AAK30562.1; -; Genomic_DNA.
DR EMBL; AF540657; AAN23136.1; -; Genomic_DNA.
DR EMBL; AF540658; AAN23137.1; -; Genomic_DNA.
DR EMBL; AF540659; AAN23138.1; -; Genomic_DNA.
DR EMBL; AF540660; AAN23139.1; -; Genomic_DNA.
DR PIR; G82324; G82324.
DR RefSeq; NP_230086.2; NC_002505.1.
DR RefSeq; WP_000861563.1; NZ_LT906614.1.
DR AlphaFoldDB; Q9KUT3; -.
DR SMR; Q9KUT3; -.
DR STRING; 243277.VC_0432; -.
DR DNASU; 2615693; -.
DR EnsemblBacteria; AAF93605; AAF93605; VC_0432.
DR GeneID; 57739170; -.
DR GeneID; 66938865; -.
DR KEGG; vch:VC_0432; -.
DR PATRIC; fig|243277.26.peg.406; -.
DR eggNOG; COG0039; Bacteria.
DR HOGENOM; CLU_047181_1_0_6; -.
DR OMA; MGWTSQA; -.
DR Proteomes; UP000000584; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0030060; F:L-malate dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniRule.
DR CDD; cd01337; MDH_glyoxysomal_mitochondrial; 1.
DR Gene3D; 3.90.110.10; -; 1.
DR HAMAP; MF_01516; Malate_dehydrog_1; 1.
DR InterPro; IPR001557; L-lactate/malate_DH.
DR InterPro; IPR022383; Lactate/malate_DH_C.
DR InterPro; IPR001236; Lactate/malate_DH_N.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR001252; Malate_DH_AS.
DR InterPro; IPR010097; Malate_DH_type1.
DR InterPro; IPR023958; Malate_DH_type1_bac.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF02866; Ldh_1_C; 1.
DR Pfam; PF00056; Ldh_1_N; 1.
DR PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR SUPFAM; SSF56327; SSF56327; 1.
DR TIGRFAMs; TIGR01772; MDH_euk_gproteo; 1.
DR PROSITE; PS00068; MDH; 1.
PE 3: Inferred from homology;
KW NAD; Oxidoreductase; Reference proteome; Tricarboxylic acid cycle.
FT CHAIN 1..311
FT /note="Malate dehydrogenase"
FT /id="PRO_0000113328"
FT ACT_SITE 177
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 7..13
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 34
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 81
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 87
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 94
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 117..119
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 119
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 153
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 227
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT VARIANT 63
FT /note="P -> T (in strain: M551)"
FT VARIANT 171
FT /note="V -> L (in strain: M553, M535, CO391, CO407 and 653/
FT 36)"
FT VARIANT 204
FT /note="K -> N (in strain: M548)"
FT VARIANT 223
FT /note="A -> T (in strain: 569B)"
FT VARIANT 277
FT /note="A -> E (in strain: M550, M551, M554, M557, M558 and
FT GD98200)"
SQ SEQUENCE 311 AA; 32015 MW; 5AD037C0E548BE9C CRC64;
MKVAVIGAAG GIGQALALLL KNRLPAGSDL ALYDIAPVTP GVAADLSHIP TPVTIKGYAG
EDPTPALEGA DVVLVSAGVA RKPGMDRADL FNVNAGIVKA LAEKIAVVCP KACVGIITNP
VNTTVPIAAE VLKKAGVYDK RKLFGVTTLD VIRSETFVAA LKDKDPGQVR VPVIGGHSGV
TILPLLSQVE GVSFTDEEVA ALTKRIQNAG TEVVEAKAGG GSATLSMGQA ACRFGLALVK
ALQGESDVVE YAYVEGEGEY APFFAQPIKL GKNGVEALLD IGKLSAYEQA ALDGMLDTLK
GDIQIGVEFV K
