MDIII_MUSDO
ID MDIII_MUSDO Reviewed; 1174 AA.
AC P0DP78; A0A1Y0AWT1;
DT 30-AUG-2017, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2017, sequence version 1.
DT 23-FEB-2022, entry version 12.
DE RecName: Full=Male determiner protein Mdmd(III) {ECO:0000303|PubMed:28495751};
DE AltName: Full=Male determiner encoded on chromosome III {ECO:0000305};
GN Name=Mdmd {ECO:0000303|PubMed:28495751};
OS Musca domestica (House fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Muscoidea;
OC Muscidae; Musca.
OX NCBI_TaxID=7370;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=28495751; DOI=10.1126/science.aam5498;
RA Sharma A., Heinze S.D., Wu Y., Kohlbrenner T., Morilla I., Brunner C.,
RA Wimmer E.A., van de Zande L., Robinson M.D., Beukeboom L.W., Bopp D.;
RT "Male sex in houseflies is determined by Mdmd, a paralog of the generic
RT splice factor gene CWC22.";
RL Science 356:642-645(2017).
CC -!- FUNCTION: Male determiner protein (M-factor) that controls male somatic
CC sexual differentiation (PubMed:28495751). Acts as a dominant factor
CC that regulates the mRNA splicing of transformer (tra) and doublesex
CC (dsx) transcripts and promotes expression of male splice forms of tra
CC and dsx (PubMed:28495751). Probably acts as a component of the
CC spliceosome C complex required for mRNA splicing factor and exon-
CC junction complex (EJC) assembly (By similarity). Hinders eIF4AIII from
CC non-specifically binding RNA and escorts it to the splicing machinery
CC to promote EJC assembly on mature mRNAs (By similarity).
CC {ECO:0000250|UniProtKB:Q9HCG8, ECO:0000269|PubMed:28495751}.
CC -!- SUBUNIT: Component of the spliceosome C complex.
CC {ECO:0000250|UniProtKB:Q9HCG8}.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000250|UniProtKB:Q9HCG8}.
CC -!- DEVELOPMENTAL STAGE: Specifically expressed in early male embryos.
CC Zygotic expression first appears in 2- to 3-hour-old embryos
CC (cellularized blastoderm stage). Expression is then maintained
CC throughout male development until adulthood.
CC {ECO:0000269|PubMed:28495751}.
CC -!- DISRUPTION PHENOTYPE: Complete sex reversal to fertile females because
CC flies exclusively express the female splice variants of transformer
CC (tra) and doublesex (dsx). {ECO:0000269|PubMed:28495751}.
CC -!- MISCELLANEOUS: The M.domestica genome only codes for one male
CC determiner Mdmd protein, which is encoded in the M region, and can be
CC located at different loci on the genome (chromosomes II, III, V or Y).
CC The M region contains a cluster of tandemly repeated Mdmd copies with
CC only one intact copy: other copies are degenerate with large
CC truncations and frameshifts and are assumed to be non-functional. The
CC presence of multiple copies in the M region may preserve its integrity
CC in a hostile non-recombining environment. This protein is encoded on
CC chromosome III. {ECO:0000269|PubMed:28495751}.
CC -!- SIMILARITY: Belongs to the CWC22 family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=It's a thin line - Issue 201
CC of March 2018;
CC URL="https://web.expasy.org/spotlight/back_issues/201/";
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DR EMBL; KY020049; ART29447.1; -; Genomic_DNA.
DR VEuPathDB; VectorBase:MDOA000598; -.
DR Proteomes; UP000095301; Whole Genome Shotgun Assembly.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0030238; P:male sex determination; IDA:UniProtKB.
DR GO; GO:0046661; P:male sex differentiation; IDA:UniProtKB.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0048024; P:regulation of mRNA splicing, via spliceosome; IMP:UniProtKB.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR003891; Initiation_fac_eIF4g_MI.
DR InterPro; IPR003890; MIF4G-like_typ-3.
DR Pfam; PF02847; MA3; 1.
DR SMART; SM00544; MA3; 1.
DR SMART; SM00543; MIF4G; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS51366; MI; 1.
PE 2: Evidence at transcript level;
KW Differentiation; mRNA processing; mRNA splicing; Nucleus;
KW Sexual differentiation.
FT CHAIN 1..1174
FT /note="Male determiner protein Mdmd(III)"
FT /id="PRO_0000441322"
FT DOMAIN 348..531
FT /note="MIF4G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT DOMAIN 641..757
FT /note="MI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT REGION 1..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 80..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 163..259
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 845..1045
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1096..1131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 88..105
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 179..206
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 207..228
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 229..259
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 845..861
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 871..905
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 906..921
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 922..955
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 966..997
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1009..1028
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1103..1119
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1174 AA; 134995 MW; 70FFD1857E9CC15D CRC64;
MNATDAESRK PENKPSSESS SSGSTSGSSD GEVSSKTYFK NNKSKVLSGQ REVVLEVVRD
LSYTICKEAE EKLVERFPRK DGSNEMLPKE DSINTNHNYT TDSNEHPVEL TTKTEECKNT
EKTKKKSFVR ALSKDKQLSA YRSRSRSTRL SYSGHISRTH SVEKSLSRYK KSVLRNRRTS
FGHGRDSSTT KRSVSRDKDN RLRRRIGSSR SHTRSHSRFR RSEKKLPSRS PRRIRSQERR
HERRRSMSSD YERIALRRSE PIKRRDKDEF FKNNKKVSGD IKKGKGNDNG TVAELEAKIT
ERQRKSLDIL TSRTGGACLT PDKLRMIQAE ITDKSSAAYQ SIAREALKKY IHGYINKVNV
DSVAVITRKL LKDNIVRGRG VLCHSIIQAQ ATSPTFTHVY AAMVAIINSK FPNIGELLLK
RLVIQFKRAF GCNDKTVCLT SSHFIAHLVN QRVAHEILAL EILTLLIESP TDDNVEVAIT
FLKECGMKLT EVSSDRVGGI FELLKNILHQ GKLDKRVQYM IKVLFQVRRD GFKDHQSIIE
SLELVEEYAQ FTHLLLLEDV TYPKDILNEF KFDDQYETNE EKYKALSKDI LGSHASDSDG
SFGSGSNSET ALSDCDKVKN EVNDKYTSGD IIDETKPNLI ALRRTIYLTL NSCLDYEECA
QKLMKTQLKT CQQNEFCQIL LDCCAEQRTY EKFYGLLTHR ICKMNKSFIE PFKEIFKDIC
QTTHCLDTNR LRNISKFFAH LLFTDAISWD VLDCIKLTED EAITSRCIFI KSFFQELVEY
MGLYHFNKKL KTEVLAGTLA GLFPKDNPRN IRFSINFFTS IGLGGITNEL CQLLKIAPKS
APSSSSSSSL SSELSAPSDD DSSSDSENKK KHKGKNKKMT KKKNPSKKKE KTKKFVGKNK
IAAKNKTIKR RTDKDNSSSK DNFLKSESSS NESISLDSLS SELFAPSSYS SSESSNDSES
KEKHKGKNKK MTKKKNPSNK KEKTKKKLSK NKKAPNKNTK KRMTEKDISS SESSISESKS
LNCSASNQNE NEKRKKRVTS KSRTKRVKMF KQCQWVDADN QRDIKRKKRA EYRYEPLVYR
KRNEECLKKG APNCXKDNYG NRQNHEISQR HDSEIKRRRE ERKKRHHEKN HSREYKRSKL
GLCQREYFLY MCCQFYYPCT FQCLCQNCHF TFYS
