MDJ1_YEAST
ID MDJ1_YEAST Reviewed; 511 AA.
AC P35191; D6VTL4;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 191.
DE RecName: Full=DnaJ homolog 1, mitochondrial;
DE Flags: Precursor;
GN Name=MDJ1; OrderedLocusNames=YFL016C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204511 / S288c / AB972;
RA Rowley N.K., Prip-Buus C., Westermann B., Brown C.M., Schwarz E.,
RA Barrell B.G., Neupert W.;
RL Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204511 / S288c / AB972;
RA Barrell B.G., Churcher C., Rajandream M.A.;
RL Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7670463; DOI=10.1038/ng0795-261;
RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA Eki T.;
RT "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT cerevisiae.";
RL Nat. Genet. 10:261-268(1995).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP FUNCTION.
RX PubMed=8168133; DOI=10.1016/0092-8674(94)90317-4;
RA Rowley N., Prip-Buus C., Westermann B., Brown C., Schwarz E., Barrell B.,
RA Neupert W.;
RT "Mdj1p, a novel chaperone of the DnaJ family, is involved in mitochondrial
RT biogenesis and protein folding.";
RL Cell 77:249-259(1994).
RN [6]
RP FUNCTION.
RX PubMed=9353316; DOI=10.1074/jbc.272.45.28539;
RA Deloche O., Liberek K., Zylicz M., Georgopoulos C.;
RT "Purification and biochemical properties of Saccharomyces cerevisiae Mdj1p,
RT the mitochondrial DnaJ homologue.";
RL J. Biol. Chem. 272:28539-28544(1997).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Plays a role in mitochondrial biogenesis and protein folding.
CC {ECO:0000269|PubMed:8168133, ECO:0000269|PubMed:9353316}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion.
CC -!- MISCELLANEOUS: Present with 7570 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z28336; CAA82189.1; -; Genomic_DNA.
DR EMBL; Z46255; CAA86351.1; -; Genomic_DNA.
DR EMBL; D50617; BAA09222.1; -; Genomic_DNA.
DR EMBL; BK006940; DAA12424.1; -; Genomic_DNA.
DR PIR; S38898; S38898.
DR RefSeq; NP_116638.1; NM_001179950.1.
DR AlphaFoldDB; P35191; -.
DR SMR; P35191; -.
DR BioGRID; 31131; 101.
DR DIP; DIP-764N; -.
DR IntAct; P35191; 60.
DR MINT; P35191; -.
DR STRING; 4932.YFL016C; -.
DR MaxQB; P35191; -.
DR PaxDb; P35191; -.
DR PRIDE; P35191; -.
DR EnsemblFungi; YFL016C_mRNA; YFL016C; YFL016C.
DR GeneID; 850530; -.
DR KEGG; sce:YFL016C; -.
DR SGD; S000001878; MDJ1.
DR VEuPathDB; FungiDB:YFL016C; -.
DR eggNOG; KOG0715; Eukaryota.
DR GeneTree; ENSGT00960000189185; -.
DR HOGENOM; CLU_017633_0_3_1; -.
DR InParanoid; P35191; -.
DR OMA; DLHCTVT; -.
DR BioCyc; YEAST:G3O-30441-MON; -.
DR PRO; PR:P35191; -.
DR Proteomes; UP000002311; Chromosome VI.
DR RNAct; P35191; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0031314; C:extrinsic component of mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0005759; C:mitochondrial matrix; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0001671; F:ATPase activator activity; IDA:SGD.
DR GO; GO:0031072; F:heat shock protein binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051082; F:unfolded protein binding; IMP:SGD.
DR GO; GO:0006458; P:'de novo' protein folding; IMP:SGD.
DR GO; GO:0051085; P:chaperone cofactor-dependent protein refolding; IBA:GO_Central.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IMP:SGD.
DR GO; GO:0006515; P:protein quality control for misfolded or incompletely synthesized proteins; IMP:SGD.
DR GO; GO:0042026; P:protein refolding; IDA:SGD.
DR GO; GO:0009408; P:response to heat; IMP:SGD.
DR CDD; cd06257; DnaJ; 1.
DR CDD; cd10719; DnaJ_zf; 1.
DR Gene3D; 1.10.287.110; -; 1.
DR HAMAP; MF_01152; DnaJ; 1.
DR InterPro; IPR012724; DnaJ.
DR InterPro; IPR002939; DnaJ_C.
DR InterPro; IPR001623; DnaJ_domain.
DR InterPro; IPR018253; DnaJ_domain_CS.
DR InterPro; IPR008971; HSP40/DnaJ_pept-bd.
DR InterPro; IPR001305; HSP_DnaJ_Cys-rich_dom.
DR InterPro; IPR036410; HSP_DnaJ_Cys-rich_dom_sf.
DR InterPro; IPR036869; J_dom_sf.
DR Pfam; PF00226; DnaJ; 1.
DR Pfam; PF01556; DnaJ_C; 1.
DR Pfam; PF00684; DnaJ_CXXCXGXG; 1.
DR PRINTS; PR00625; JDOMAIN.
DR SMART; SM00271; DnaJ; 1.
DR SUPFAM; SSF46565; SSF46565; 1.
DR SUPFAM; SSF49493; SSF49493; 2.
DR SUPFAM; SSF57938; SSF57938; 1.
DR PROSITE; PS00636; DNAJ_1; 1.
DR PROSITE; PS50076; DNAJ_2; 1.
DR PROSITE; PS51188; ZF_CR; 1.
PE 1: Evidence at protein level;
KW Chaperone; Metal-binding; Mitochondrion; Reference proteome; Repeat;
KW Stress response; Transit peptide; Zinc; Zinc-finger.
FT TRANSIT 1..55
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 56..511
FT /note="DnaJ homolog 1, mitochondrial"
FT /id="PRO_0000007268"
FT DOMAIN 59..127
FT /note="J"
FT REPEAT 230..237
FT /note="CXXCXGXG motif"
FT REPEAT 247..254
FT /note="CXXCXGXG motif"
FT REPEAT 269..276
FT /note="CXXCXGXG motif"
FT REPEAT 285..292
FT /note="CXXCXGXG motif"
FT ZN_FING 217..297
FT /note="CR-type"
SQ SEQUENCE 511 AA; 55561 MW; 007343427C66D2B6 CRC64;
MAFQQGVLSR CSGVFRHHVG HSRHINNILY RHAIAFASIA PRIPKSSFHT SAIRNNEAFK
DPYDTLGLKK SATGAEIKKA YYKLAKKYHP DINKEPDAEK KFHDLQNAYE ILSDETKRQQ
YDQFGPAAFG GGGAAGGAGG GSGSPFGSQF HDFSGFTSAG GSPFGGINFE DLFGAAFGGG
GRGSGGASRS SSMFRQYRGD PIEIVHKVSF KDAVFGSKNV QLRFSALDPC STCSGTGMKP
NTHKVSCSTC HGTGTTVHIR GGFQMMSTCP TCNGEGTMKR PQDNCTKCHG EGVQVNRAKT
ITVDLPHGLQ DGDVVRIPGQ GSYPDIAVEA DLKDSVKLSR GDILVRIRVD KDPNFSIKNK
YDIWYDKEIP ITTAALGGTV TIPTVEGQKI RIKVAPGTQY NQVISIPNMG VPKTSTIRGD
MKVQYKIVVK KPQSLAEKCL WEALADVTND DMAKKTMQPG TAAGTAINEE ILKKQKQEEE
KHAKKDDDNT LKRLENFITN TFRKIKGDKK N
