MDLA_PENCY
ID MDLA_PENCY Reviewed; 305 AA.
AC P61869; P25234;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2004, sequence version 1.
DT 25-MAY-2022, entry version 50.
DE RecName: Full=Mono- and diacylglycerol lipase;
DE Short=MDGL;
DE EC=3.1.1.-;
DE Flags: Precursor;
GN Name=mdlA;
OS Penicillium cyclopium.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX NCBI_TaxID=60167;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PG37;
RA Wu M.C., Li J.H., Qian Z.K., Min T.S., Sun C.R., Huang W.D.;
RT "Cloning and structure of the mono- and diacylglycerol lipase-encoding gene
RT from Penicillium cyclopium PG37.";
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Hydrolyzes mono- and diacylglycerol but not triacylglycerol.
CC -!- PTM: Multiple forms of this lipase are due to the presence of different
CC carbohydrates, which may contribute to the stability of this lipase but
CC not to the enzyme activity.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
CC {ECO:0000305}.
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DR EMBL; AF288219; AAF99710.1; -; Genomic_DNA.
DR PIR; JQ1188; JQ1188.
DR PDB; 5CH8; X-ray; 1.62 A; A=27-305.
DR PDBsum; 5CH8; -.
DR AlphaFoldDB; P61869; -.
DR SMR; P61869; -.
DR ESTHER; penca-mdgli; Lipase_3.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002921; Fungal_lipase-like.
DR InterPro; IPR005592; Mono/diacylglycerol_lipase_N.
DR Pfam; PF03893; Lipase3_N; 1.
DR Pfam; PF01764; Lipase_3; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00120; LIPASE_SER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Glycoprotein; Hydrolase; Lipid degradation;
KW Lipid metabolism; Signal; Zymogen.
FT SIGNAL 1..26
FT /evidence="ECO:0000250"
FT CHAIN 27..302
FT /note="Mono- and diacylglycerol lipase"
FT /id="PRO_0000017762"
FT PROPEP 303..305
FT /note="Removed in mature form"
FT /evidence="ECO:0000250"
FT /id="PRO_0000017763"
FT ACT_SITE 171
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:P61870"
FT ACT_SITE 225
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:P61870"
FT ACT_SITE 285
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:P61870"
FT CARBOHYD 251
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 62..67
FT /evidence="ECO:0000250|UniProtKB:P61870"
FT DISULFID 129..132
FT /evidence="ECO:0000250|UniProtKB:P61870"
FT HELIX 30..45
FT /evidence="ECO:0007829|PDB:5CH8"
FT HELIX 49..52
FT /evidence="ECO:0007829|PDB:5CH8"
FT HELIX 68..72
FT /evidence="ECO:0007829|PDB:5CH8"
FT STRAND 76..81
FT /evidence="ECO:0007829|PDB:5CH8"
FT STRAND 90..96
FT /evidence="ECO:0007829|PDB:5CH8"
FT TURN 97..100
FT /evidence="ECO:0007829|PDB:5CH8"
FT STRAND 101..107
FT /evidence="ECO:0007829|PDB:5CH8"
FT HELIX 112..118
FT /evidence="ECO:0007829|PDB:5CH8"
FT STRAND 127..129
FT /evidence="ECO:0007829|PDB:5CH8"
FT HELIX 136..159
FT /evidence="ECO:0007829|PDB:5CH8"
FT STRAND 164..170
FT /evidence="ECO:0007829|PDB:5CH8"
FT HELIX 172..185
FT /evidence="ECO:0007829|PDB:5CH8"
FT TURN 186..188
FT /evidence="ECO:0007829|PDB:5CH8"
FT STRAND 193..197
FT /evidence="ECO:0007829|PDB:5CH8"
FT HELIX 205..214
FT /evidence="ECO:0007829|PDB:5CH8"
FT STRAND 217..222
FT /evidence="ECO:0007829|PDB:5CH8"
FT HELIX 227..229
FT /evidence="ECO:0007829|PDB:5CH8"
FT HELIX 233..235
FT /evidence="ECO:0007829|PDB:5CH8"
FT STRAND 243..246
FT /evidence="ECO:0007829|PDB:5CH8"
FT HELIX 256..258
FT /evidence="ECO:0007829|PDB:5CH8"
FT STRAND 259..262
FT /evidence="ECO:0007829|PDB:5CH8"
FT STRAND 264..266
FT /evidence="ECO:0007829|PDB:5CH8"
FT HELIX 270..274
FT /evidence="ECO:0007829|PDB:5CH8"
FT HELIX 279..285
FT /evidence="ECO:0007829|PDB:5CH8"
FT STRAND 287..291
FT /evidence="ECO:0007829|PDB:5CH8"
FT TURN 292..295
FT /evidence="ECO:0007829|PDB:5CH8"
SQ SEQUENCE 305 AA; 32948 MW; 42C2A16203DBA1AA CRC64;
MRLSFFTALS AVASLGYALP GKLQSRDVST SELDQFEFWV QYAAASYYEA DYTAQVGDKL
SCSKGNCPEV EATGATVSYD FSDSTITDTA GYIAVDHTNS AVVLAFRGSY SVRNWVADAT
FVHTNPGLCD GCLAELGFWS SWKLVRDDII KELKEVVAQN PNYELVVVGH SLGAAVATLA
ATDLRGKGYP SAKLYAYASP RVGNAALAKY ITAQGNNFRF THTNDPVPKL PLLSMGYVHV
SPEYWITSPN NATVSTSDIK VIDGDVSFDG NTGTGLPLLT DFEAHIWYFV QVDAGKGPGL
PFKRV
