MDM1_YEAST
ID MDM1_YEAST Reviewed; 1127 AA.
AC Q01846; D6W0I0; Q04196;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2001, sequence version 3.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Structural protein MDM1;
DE AltName: Full=Mitochondrial distribution and morphology protein 1;
GN Name=MDM1; OrderedLocusNames=YML104C; ORFNames=YM8339.15C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 633-1127, FUNCTION, AND SUBCELLULAR
RP LOCATION.
RX PubMed=1378448; DOI=10.1083/jcb.118.2.385;
RA McConnell S.J., Yaffe M.P.;
RT "Nuclear and mitochondrial inheritance in yeast depends on novel
RT cytoplasmic structures defined by the MDM1 protein.";
RL J. Cell Biol. 118:385-395(1992).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 874-1127.
RA Stirling C.J.;
RL Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-670; SER-673 AND SER-692, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Essential for mitotic growth. Mediates organelle inheritance.
CC {ECO:0000269|PubMed:1378448}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:1378448}.
CC -!- MISCELLANEOUS: Present with 784 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the sorting nexin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA47014.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA89114.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X80835; CAA56793.1; -; Genomic_DNA.
DR EMBL; Z49210; CAA89114.1; ALT_INIT; Genomic_DNA.
DR EMBL; X66371; CAA47014.1; ALT_FRAME; Genomic_DNA.
DR EMBL; X65783; CAA46664.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09794.1; -; Genomic_DNA.
DR PIR; S47445; S47445.
DR RefSeq; NP_013603.1; NM_001182466.1.
DR AlphaFoldDB; Q01846; -.
DR SMR; Q01846; -.
DR BioGRID; 35039; 119.
DR DIP; DIP-810N; -.
DR IntAct; Q01846; 2.
DR MINT; Q01846; -.
DR STRING; 4932.YML104C; -.
DR iPTMnet; Q01846; -.
DR MaxQB; Q01846; -.
DR PaxDb; Q01846; -.
DR PRIDE; Q01846; -.
DR EnsemblFungi; YML104C_mRNA; YML104C; YML104C.
DR GeneID; 854867; -.
DR KEGG; sce:YML104C; -.
DR SGD; S000004572; MDM1.
DR VEuPathDB; FungiDB:YML104C; -.
DR eggNOG; KOG2101; Eukaryota.
DR GeneTree; ENSGT00950000182856; -.
DR HOGENOM; CLU_002131_1_0_1; -.
DR InParanoid; Q01846; -.
DR OMA; AMYVVEV; -.
DR BioCyc; YEAST:G3O-32688-MON; -.
DR PRO; PR:Q01846; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q01846; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0071561; C:nucleus-vacuole junction; IDA:SGD.
DR GO; GO:0035091; F:phosphatidylinositol binding; IBA:GO_Central.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:SGD.
DR GO; GO:0000001; P:mitochondrion inheritance; IMP:SGD.
DR GO; GO:1990854; P:vacuole-ER tethering; IMP:SGD.
DR Gene3D; 3.30.1520.10; -; 1.
DR InterPro; IPR003114; Phox_assoc.
DR InterPro; IPR001683; PX_dom.
DR InterPro; IPR036871; PX_dom_sf.
DR InterPro; IPR013937; Sorting_nexin_C.
DR Pfam; PF08628; Nexin_C; 1.
DR Pfam; PF00787; PX; 1.
DR Pfam; PF02194; PXA; 1.
DR SMART; SM00312; PX; 1.
DR SMART; SM00313; PXA; 1.
DR SUPFAM; SSF64268; SSF64268; 1.
DR PROSITE; PS50195; PX; 1.
DR PROSITE; PS51207; PXA; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..1127
FT /note="Structural protein MDM1"
FT /id="PRO_0000096330"
FT DOMAIN 85..273
FT /note="PXA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00147,
FT ECO:0000255|PROSITE-ProRule:PRU00553"
FT DOMAIN 782..905
FT /note="PX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00147"
FT COILED 705..762
FT /evidence="ECO:0000255"
FT MOD_RES 670
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 673
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 692
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT CONFLICT 635
FT /note="H -> D (in Ref. 3; CAA47014)"
FT /evidence="ECO:0000305"
FT CONFLICT 772
FT /note="Q -> R (in Ref. 3; CAA47014)"
FT /evidence="ECO:0000305"
FT CONFLICT 899
FT /note="T -> I (in Ref. 3; CAA47014)"
FT /evidence="ECO:0000305"
FT CONFLICT 1063
FT /note="R -> K (in Ref. 3; CAA47014)"
FT /evidence="ECO:0000305"
FT CONFLICT 1081
FT /note="T -> S (in Ref. 3; CAA47014)"
FT /evidence="ECO:0000305"
FT CONFLICT 1096
FT /note="G -> R (in Ref. 3; CAA47014)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1127 AA; 129981 MW; 7C9177B87FDAF512 CRC64;
MPKFPQFRLI LVLFYLISMI QWSVITFSLG FFLNVCIFAY FVFFKSLPDL PKPQPRFVDI
VPESSNTVDV DKELKSVEGL IQDGNAQIGK ELESIVNLII KDFVQPWFTK IDKNSDAEFL
KVIKWRLLQT LLVVKDKLMK NDSASLIVLK LLPIFNKHFS TFCDAREAVL SDLTLERHKS
ANIDLQIAVE FNKNYKIHKS LSLKPNALQK EIEKSIRKTV IGLLPHLFDN DELDSLLVFT
LMTEVLTTCI ISPLIFKFTD PDSWNLRIVS LSQNYFEEKH KVHKIRRMLS KELQDHRKVM
NDVANKDVGE PSSEKLELNA EYTGKQFEHY LNQLDSLLDL SDIKYVAYSL ALKIYQLKEN
EHLTKENLKY KKRLLLSLNL IESKLSFPGS EIDTASKKLA REANYPDLNM DNGIVLKEMA
SFLTSITLKD IVDDSEFLPF FESFLGSVPE TQGSTFLEYS QTIESFKNPL EDATSEDIIS
GYSGISTMQL QEISSKFFHN NNLQNMKLLD EGLVKNIILF RNSFQINNDE DTFILARKSV
LLLQTEAIKY LDDRFLPLFK KTPSFLKMLS TSHIISTDIY AHFLSRIGGV NNPEQNKIIK
DNVKTDFMNP VRIFANPGIT DALDNIVNGS GSKPHKSRIS SNPRYSQLFG SENDNIFKDK
LFDDENDNTS EISVVEDQLD HPRNMEKVSV SSGNSGLNPS QFYGSNNFRD NIASLTISID
QIEKELELLR HLILKADLTN NQMQLKILKK SQRTLLKELE MKELLKQQYM VQENGNSLFR
KTKIYIRSYF SENSSNGLKE ITYYIINIHH FNNGQVSSWD MARRYNEFFE LNTYLKKNFR
DLMRQLQDLF PSKVKMSLKY HVTKTLLYEE RKQKLEKYLR ELLSISEICE DNIFRRFLTD
PTPFKLNKEY MHDDILEEPL HEPIGSSNST SNSSSVVDLQ SSEDGGELNF YEDERHFFTD
SGYPFYSQNK SFVKQICDLF ISLFALNKAN AGWLRGRAII TVLQQLLGST IEKYIKVSIQ
KLRSEDQVFE AIVTFKNMLW GDNGLFERKR NETAEATRSE GERLRTEQLA LTSLQRLFAD
TCGRVVGLRD SHEAAGRVHA MLQNPYLNAS LLLEALDAIL LDIICND
