MDM32_YEAST
ID MDM32_YEAST Reviewed; 622 AA.
AC Q12171; D6W2K4;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Mitochondrial distribution and morphology protein 32;
DE Flags: Precursor;
GN Name=MDM32; OrderedLocusNames=YOR147W; ORFNames=O3521;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c / FY1678;
RX PubMed=9046089;
RX DOI=10.1002/(sici)1097-0061(199701)13:1<73::aid-yea52>3.0.co;2-m;
RA Bordonne R., Camasses A., Madania A., Poch O., Tarassov I.A., Winsor B.,
RA Martin R.P.;
RT "Analysis of a 35.6 kb region on the right arm of Saccharomyces cerevisiae
RT chromosome XV.";
RL Yeast 13:73-83(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION.
RX PubMed=11907266; DOI=10.1091/mbc.01-12-0588;
RA Dimmer K.S., Fritz S., Fuchs F., Messerschmitt M., Weinbach N., Neupert W.,
RA Westermann B.;
RT "Genetic basis of mitochondrial function and morphology in Saccharomyces
RT cerevisiae.";
RL Mol. Biol. Cell 13:847-853(2002).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH MDM31.
RX PubMed=15631992; DOI=10.1083/jcb.200410030;
RA Dimmer K.S., Jakobs S., Vogel F., Altmann K., Westermann B.;
RT "Mdm31 and Mdm32 are inner membrane proteins required for maintenance of
RT mitochondrial shape and stability of mitochondrial DNA nucleoids in
RT yeast.";
RL J. Cell Biol. 168:103-115(2005).
RN [8]
RP IDENTIFICATION OF PROBABLE INITIATION SITE.
RX PubMed=15905473; DOI=10.1093/nar/gki583;
RA Zhang Z., Dietrich F.S.;
RT "Mapping of transcription start sites in Saccharomyces cerevisiae using 5'
RT SAGE.";
RL Nucleic Acids Res. 33:2838-2851(2005).
CC -!- FUNCTION: Involved in the organization of the mitochondrial membranes
CC and the global structure of the mitochondria. Also required for
CC mitochondrial distribution and mobility as well as for the maintenance
CC of mitochondrial DNA nucleoids structures.
CC {ECO:0000269|PubMed:11907266, ECO:0000269|PubMed:15631992}.
CC -!- SUBUNIT: Interacts with MDM31. Participates in a complex of about 175
CC kDa. {ECO:0000269|PubMed:15631992}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 10400 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the MDM31/MDM32 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC49633.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA99353.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U55020; AAC49633.1; ALT_INIT; Genomic_DNA.
DR EMBL; Z75055; CAA99353.1; ALT_INIT; Genomic_DNA.
DR EMBL; BK006948; DAA10920.1; -; Genomic_DNA.
DR PIR; S67035; S67035.
DR RefSeq; NP_014790.4; NM_001183566.3.
DR AlphaFoldDB; Q12171; -.
DR SMR; Q12171; -.
DR BioGRID; 34543; 507.
DR DIP; DIP-2556N; -.
DR IntAct; Q12171; 1.
DR MINT; Q12171; -.
DR STRING; 4932.YOR147W; -.
DR MaxQB; Q12171; -.
DR PaxDb; Q12171; -.
DR PRIDE; Q12171; -.
DR EnsemblFungi; YOR147W_mRNA; YOR147W; YOR147W.
DR GeneID; 854318; -.
DR KEGG; sce:YOR147W; -.
DR SGD; S000005673; MDM32.
DR VEuPathDB; FungiDB:YOR147W; -.
DR eggNOG; ENOG502QQU5; Eukaryota.
DR GeneTree; ENSGT00940000176498; -.
DR HOGENOM; CLU_016236_3_0_1; -.
DR InParanoid; Q12171; -.
DR OMA; FAKEMVG; -.
DR BioCyc; YEAST:G3O-33665-MON; -.
DR PRO; PR:Q12171; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q12171; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0006873; P:cellular ion homeostasis; IMP:SGD.
DR GO; GO:0000001; P:mitochondrion inheritance; IMP:SGD.
DR GO; GO:0007005; P:mitochondrion organization; IMP:SGD.
DR InterPro; IPR012571; Mdm31/Mdm32.
DR PANTHER; PTHR31068; PTHR31068; 1.
DR Pfam; PF08118; MDM31_MDM32; 2.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..70
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 71..622
FT /note="Mitochondrial distribution and morphology protein
FT 32"
FT /id="PRO_0000021665"
FT TOPO_DOM 71..123
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..601
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 602..622
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 622 AA; 72028 MW; 84FCDF143C0BB276 CRC64;
MLITRLRVPT IKRPLLPITS HLVRHCIRTY VATNHGNVRP FITPYKSSLP VRCLIAQRHI
RTFPSNDKFT TKASNIETIL LRKNNEREFK QSLLADAKNF QERFKINLKW ILIKNNRPFS
LNEISIIASW LILSQILWLI LSTTTFISFY LFVINSVFSQ EYIHEKKIYE RLLKWLLKDH
KCSNQDLEIT FSPEDKASML VLSPDWESNS ILIKRLNVRD EILDLDLKFH HINLNVSLKN
WLLGRGLITN VSIYGIRGCL NLSNFINLVN SFQGDQKTEN FLKTLNNVEI TDSEILLKQS
LSAQETPSLK FSIYNLSLPR LRLNHFISDI LSAKTFSGSI NNSLFNLFKR QQKLTAVIEN
NNKNRMASSK FDFTDNNQEN YRTVTHQDDP NYVTTLRLNF ININDLKFNG DGKFNWLKDG
QVEILADIML TNSTSHLSSE SKYAVVDLKV TCRDLKTTFP QEPPVLSTGD SIVSLDELKP
IITFINSYEG MANPILKDFS ENERLTNSII WNSPNVSINR QRKSYPLTTK VTSNSTKEII
KFHNQPNTNA NEIVLRCKMV KNLSDLQLIN INQILDQITM ELYVDLTKIV EDWEFKNKND
WMKQWGTTFA SQLLLFGFGA MV
