ID   MDMB_STRMY              Reviewed;         387 AA.
AC   Q00718;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=Acyltransferase MdmB;
DE            EC=2.3.1.-;
GN   Name=mdmB;
OS   Streptomyces mycarofaciens.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1949;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 21454 / JCM 4939 / NBRC 13792;
RX   PubMed=1629172; DOI=10.1128/jb.174.15.5141-5144.1992;
RA   Hara O., Hutchinson C.R.;
RT   "A macrolide 3-O-acyltransferase gene from the midecamycin-producing
RT   species Streptomyces mycarofaciens.";
RL   J. Bacteriol. 174:5141-5144(1992).
CC   -!- FUNCTION: Catalyzes the acylation of the mycaminose sugar during
CC       midecamycin biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the acyltransferase 3 family. {ECO:0000305}.
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DR   EMBL; M93958; AAA26781.1; -; Genomic_DNA.
DR   PIR; A42719; A42719.
DR   AlphaFoldDB; Q00718; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IEA:InterPro.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR002656; Acyl_transf_3_dom.
DR   Pfam; PF01757; Acyl_transf_3; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Antibiotic biosynthesis; Cell membrane; Membrane;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..387
FT                   /note="Acyltransferase MdmB"
FT                   /id="PRO_0000208080"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        209..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        236..256
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        258..278
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        292..312
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        336..356
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   387 AA;  42172 MW;  DD6C706BD21F793A CRC64;
     MPPRVVRLPS LTGLRWFAAL AVFACHIAQQ QFFADQQVGT ALLHITTLGS IAVSVFFLLS
     GFVLAWSARD KDSVTTFWRR RFAKIYPLHL VTFLIAGVII FSLAEPTLPG GSVWDGLVPD
     LLLVQSWLPE PTIIAGFNTP SWSLSCEFAF YLTFPLWYRL VRKIPVRRLW WCAAGIAAAV
     ICVPFVTSQF PASAETAPGM PLNELWFACW LPPVRMLEFV LGIVMALILR TGVWRGPGVV
     SSALLLAAAY GVTQVVPPMF TIAACSIVPA ALLITALANA DVQGLRTGLR SAVLVRLGEW
     SFAFYLVHFM VIRYGHRLMG GELGYARQWS TASAGALALA MLAVAIVAGG LLHTVVENPC
     MRLLGRRRPV ATAPDPATDE APKLTRA