MDN1_ARATH
ID MDN1_ARATH Reviewed; 5400 AA.
AC A0A1P8AUY4; B0FU84; F4HRR8; Q0WV24; Q9ZW94;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 1.
DT 03-AUG-2022, entry version 29.
DE RecName: Full=Midasin {ECO:0000303|PubMed:23572950};
DE Short=AtMDN1 {ECO:0000303|PubMed:23572950};
DE AltName: Full=Dynein-related AAA-ATPase MDN1;
DE AltName: Full=MIDAS-containing protein 1 {ECO:0000303|PubMed:23572950};
DE AltName: Full=Protein DWARF AND SHORT ROOT 1 {ECO:0000303|PubMed:27824150};
GN Name=MDN1 {ECO:0000303|PubMed:23572950};
GN Synonyms=DSR1 {ECO:0000303|PubMed:27824150};
GN OrderedLocusNames=At1g67120 {ECO:0000312|Araport:AT1G67120};
GN ORFNames=F5A8.11 {ECO:0000312|EMBL:AAD10657.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 706-1045 (ISOFORM 1/2).
RC STRAIN=cv. Bla-10, cv. Chi-1, cv. Co-1, cv. Columbia, cv. Cvi-0,
RC cv. Da(1)-12, cv. Di-G, cv. Landsberg erecta, cv. Li-3, cv. Mt-0,
RC cv. PHW-1, and cv. PHW-32;
RX PubMed=18273534; DOI=10.1007/s00239-007-9063-3;
RA Moore R.C., Stevens M.H.H.;
RT "Local patterns of nucleotide polymorphism are highly variable in the
RT selfing species Arabidopsis thaliana.";
RL J. Mol. Evol. 66:116-129(2008).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4477-5400 (ISOFORM 1/2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=23572950; DOI=10.1007/s12298-010-0005-y;
RA Chantha S.-C., Gray-Mitsumune M., Houde J., Matton D.P.;
RT "The MIDASIN and NOTCHLESS genes are essential for female gametophyte
RT development in Arabidopsis thaliana.";
RL Physiol. Mol. Biol. Plants 16:3-18(2010).
RN [6]
RP FUNCTION, MUTAGENESIS OF GLU-3845, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=27824150; DOI=10.1038/srep36446;
RA Li P.-C., Yu S.-W., Li K., Huang J.-G., Wang X.-J., Zheng C.-C.;
RT "The mutation of Glu at amino acid 3838 of AtMDN1 provokes pleiotropic
RT developmental phenotypes in Arabidopsis.";
RL Sci. Rep. 6:36446-36446(2016).
CC -!- FUNCTION: Nuclear chaperone required for maturation and nuclear export
CC of pre-60S ribosome subunits. Functions at successive maturation steps
CC to remove ribosomal factors at critical transition points, first
CC driving the exit of early pre-60S particles from the nucleolus and then
CC driving late pre-60S particles from the nucleus (By similarity).
CC Required for female gametophyte development (PubMed:23572950). Involved
CC in the expression regulation of genes related to plant growth and
CC development (PubMed:27824150). {ECO:0000250|UniProtKB:Q12019,
CC ECO:0000269|PubMed:23572950, ECO:0000269|PubMed:27824150}.
CC -!- SUBUNIT: Associates with pre-60S ribosomes in the nucleoplasm.
CC {ECO:0000250|UniProtKB:Q12019}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q12019}. Nucleus, nucleoplasm
CC {ECO:0000250|UniProtKB:Q12019}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A0A1P8AUY4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A0A1P8AUY4-2; Sequence=VSP_059112;
CC -!- TISSUE SPECIFICITY: Constitutively and ubiquitously expressed
CC (PubMed:23572950). Mostly observed in the shoot apex and root tip, and,
CC to a lower extent, in mature seeds, seedling (excluding the hypocotyl),
CC roots, stems, leaves and flowers (PubMed:27824150).
CC {ECO:0000269|PubMed:23572950, ECO:0000269|PubMed:27824150}.
CC -!- DISRUPTION PHENOTYPE: Female semisterility due to strongly delayed
CC development of female gametophyte. {ECO:0000269|PubMed:23572950}.
CC -!- SIMILARITY: Belongs to the midasin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD10657.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004146; AAD10657.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34598.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60463.1; -; Genomic_DNA.
DR EMBL; EU351100; ABY68285.1; -; Genomic_DNA.
DR EMBL; EU351113; ABY68324.1; -; Genomic_DNA.
DR EMBL; EU351101; ABY68288.1; -; Genomic_DNA.
DR EMBL; EU351102; ABY68291.1; -; Genomic_DNA.
DR EMBL; EU351103; ABY68294.1; -; Genomic_DNA.
DR EMBL; EU351104; ABY68297.1; -; Genomic_DNA.
DR EMBL; EU351105; ABY68300.1; -; Genomic_DNA.
DR EMBL; EU351106; ABY68303.1; -; Genomic_DNA.
DR EMBL; EU351107; ABY68306.1; -; Genomic_DNA.
DR EMBL; EU351109; ABY68312.1; -; Genomic_DNA.
DR EMBL; EU351110; ABY68315.1; -; Genomic_DNA.
DR EMBL; EU351111; ABY68318.1; -; Genomic_DNA.
DR EMBL; AK226955; BAE99024.1; -; mRNA.
DR PIR; B96695; B96695.
DR RefSeq; NP_001322748.1; NM_001334273.1. [A0A1P8AUY4-1]
DR RefSeq; NP_176883.5; NM_105382.7. [A0A1P8AUY4-2]
DR STRING; 3702.AT1G67120.1; -.
DR iPTMnet; A0A1P8AUY4; -.
DR ProteomicsDB; 228841; -. [A0A1P8AUY4-1]
DR EnsemblPlants; AT1G67120.1; AT1G67120.1; AT1G67120. [A0A1P8AUY4-2]
DR EnsemblPlants; AT1G67120.2; AT1G67120.2; AT1G67120. [A0A1P8AUY4-1]
DR GeneID; 843032; -.
DR Gramene; AT1G67120.1; AT1G67120.1; AT1G67120. [A0A1P8AUY4-2]
DR Gramene; AT1G67120.2; AT1G67120.2; AT1G67120. [A0A1P8AUY4-1]
DR KEGG; ath:AT1G67120; -.
DR Araport; AT1G67120; -.
DR TAIR; locus:2033661; AT1G67120.
DR eggNOG; KOG1808; Eukaryota.
DR OMA; ELGPPNI; -.
DR OrthoDB; 138219at2759; -.
DR PRO; PR:A0A1P8AUY4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; A0A1P8AUY4; baseline and differential.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009553; P:embryo sac development; IMP:UniProtKB.
DR GO; GO:0048638; P:regulation of developmental growth; IMP:UniProtKB.
DR GO; GO:2000200; P:regulation of ribosomal subunit export from nucleus; IBA:GO_Central.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IBA:GO_Central.
DR GO; GO:0000055; P:ribosomal large subunit export from nucleus; IMP:TAIR.
DR GO; GO:0006364; P:rRNA processing; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 7.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR040848; AAA_lid_7.
DR InterPro; IPR011704; ATPase_dyneun-rel_AAA.
DR InterPro; IPR012099; Midasin.
DR InterPro; IPR041190; Midasin_AAA_lid_5.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR Pfam; PF07728; AAA_5; 7.
DR Pfam; PF17865; AAA_lid_5; 1.
DR Pfam; PF17867; AAA_lid_7; 3.
DR PIRSF; PIRSF010340; Midasin; 1.
DR SMART; SM00382; AAA; 6.
DR SUPFAM; SSF52540; SSF52540; 6.
DR SUPFAM; SSF53300; SSF53300; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS50234; VWFA; 1.
PE 1: Evidence at protein level;
KW Abscisic acid signaling pathway; Alternative splicing; ATP-binding;
KW Chaperone; Coiled coil; Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..5400
FT /note="Midasin"
FT /id="PRO_0000441781"
FT DOMAIN 5186..5387
FT /note="VWFA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT REGION 345..571
FT /note="AAA-ATPase protomer 1"
FT /evidence="ECO:0000255"
FT REGION 656..986
FT /note="AAA-ATPase protomer 2"
FT /evidence="ECO:0000255"
FT REGION 1050..1308
FT /note="AAA-ATPase protomer 3"
FT /evidence="ECO:0000255"
FT REGION 1347..1652
FT /note="AAA-ATPase protomer 4"
FT /evidence="ECO:0000255"
FT REGION 1769..2023
FT /note="AAA-ATPase protomer 5"
FT /evidence="ECO:0000255"
FT REGION 2074..2347
FT /note="AAA-ATPase protomer 6"
FT /evidence="ECO:0000255"
FT REGION 2435..4569
FT /note="Linker"
FT /evidence="ECO:0000250|UniProtKB:Q12019"
FT REGION 4540..4891
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4905..4929
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4990..5069
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 2896..2916
FT /evidence="ECO:0000255"
FT COILED 3233..3253
FT /evidence="ECO:0000255"
FT COILED 3896..3916
FT /evidence="ECO:0000255"
FT COILED 5271..5291
FT /evidence="ECO:0000255"
FT MOTIF 5157..5164
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 4566..4609
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4610..4631
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4668..4706
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4707..4721
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4741..4755
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4756..4781
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4796..4834
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4841..4891
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4994..5009
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5026..5043
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 360..367
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00136"
FT BINDING 674..681
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00136"
FT BINDING 1079..1086
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00136"
FT BINDING 1369..1376
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00136"
FT BINDING 1786..1793
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00136"
FT BINDING 2095..2102
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00136"
FT VAR_SEQ 3197..3203
FT /note="Missing (in isoform 2)"
FT /id="VSP_059112"
FT MUTAGEN 3845
FT /note="E->K: In dsr1; pleiotropic developmental phenotypes
FT including slow germination, short root, dwarf shoot, and
FT reduced seed set under normal growth conditions. Impaired
FT expression of genes related to plant growth and
FT development."
FT /evidence="ECO:0000269|PubMed:27824150"
SQ SEQUENCE 5400 AA; 611889 MW; B6091B43B9EC35E1 CRC64;
MAIDGSFNLK LALETFSVRC PKVAAFPCFT SILSKGGEVV DNEEVIHALG DAFLHPEFTV
PLVHCFLPII RNVVDRVVGL LRLVDDLKSS IDYSDDVSSV LDNAMTEGIS VIDFYVRRGQ
RLELHECACL AFSRALHFNT SLLGSILNYF EKAPPPYERI LVKDIVSESR MEATDAYLLC
LRVSYRFLVI RPEVFSKLWD WSCYLDSMKR LSECPRQQRH FLEKYRDAVW CGIQILSVVL
RCSDRLAGCF GFEEEEALSC LLRWEEFCQD IEIEKAGLYI QLPTYTALKS LQQFNTLVPG
INKRQSAGLE ADEPQMKIRR LDTWDVNSFS EPFEIHSRVK KSFEMVSLAV SQKRPVLLYG
PSGSGKSALI RKLADESGNH VVFIHMDDQL DGKTLVGTYV CTDQPGEFRW QPGSLTQAIM
NGFWVVLEDI DKAPSDVPLV LSSLLGGSCS FLTSQGEEIR IAETFQLFST ISTPECSVSH
IRDAGNSLSP LWRRIVVYPP DRESLQSILG ARYPNLGPVA EKLIETFETI NSALRPQFSS
STTENSATFS SPSRFSLRDL LKWCERVHGL PSYDGHAVYQ EAADIFSASN MSVKNRVAVS
EIVASIWNVA VPESQDKPPI QEFSGILKIG RVSLPLGETA SHDRSRFVET RTSTRLLEKI
ARSVEYNEPV LLVGETGTGK TTLVQNLAHW IGQKLTVLNL SQQSDIVDLL GGFKPIDPKL
MCTMVYNEFN ELARDLKIKD DSKIMKWLQD NFRAKKWHTF LTGLLDIIKG IEGRITERME
GKIGEARSRS GRKRKKPEEE LKNCACLRTK VNKIRQQIHS GGMVFTFVEG AFVTALREGH
WVLLDEVNLA PPEILGRLIG VLEGVRGSLC LAERGDVMGI PRHLNFRLFA CMNPATDAGK
RDLPFSFRSR FTEYAVDDDI CDDDLEIFVR RFLGGRGSDS KLVANIVWFY KEAKRLSEES
LQDGANQKPQ YSLRSLYRAL EYAIKAEAIG GFQKALYDGF SMFFLSLLDA SSAKIVEPII
KRISGENIRS QPLQRYLGEL KGSSDKFVGS YVKTKSVIDH LNHLAHAIFI KRYPVLLQGP
TSSGKTSLVK YLAAISGNKF VRINNHEQTD IQEYLGSYMT DSSGKLVFHE GALVKAVRGG
HWIVLDELNL APSDVLEALN RLLDDNRELF VPELSETISA HPNFMLFATQ NPPTLYGGRK
ILSRAFRNRF VEIHVDEIPE DELSEILTTK CSIANSHASK MVEVMKDLQR NRQSSKAFAG
KHGYITPRDL FRWAYRFRTY DGTSHEELAR EGYYILAERL RDDTEKVVVQ EVLERHFRVS
LAKDDLYNME LPRLDSIQNR KFTWTQSMRR LFFLIDRSYK LREPVLLVGD TGGGKTTICQ
ILSDVKKKRL HILNCHQYTE TSDFLGGFFP VRDRSKLITE YENQVKQLEL SQALTPFGQD
IVICGDISRA EVSIKSVEVA LEKYKNGSVI GVAATPQDVD FLEKIRNNMV MLYQKWRAIF
VWQDGPLVEA MRAGNIVLVD EISLADDSVL ERMNSVLETD RKLSLAEKGG PVLEEVVAHE
DFFVLATMNP GGDYGKKELS PALRNRFTEI WVPPITDTEE LRSIAFSGLS SLKESNVVDP
IINFWEWFNR LHTGRTLTVR DLLSWVAFVN MATESLGPAY AILHGAFLVL LDGLSLGTGF
SGRDGQDLRE KCFAFLLQQL ELFASDTLPL ELSRMELYGW GDSKAICEKS KSVRHEGMFG
IDPFFISKGD ENPEIGGFEF LAPTTHRNVL RVLRAMQLSK PILLEGSPGV GKTSLILALG
KYSGHKVVRI NLSEQTDMMD LLGSDLPVES DEDMKFAWSD GILLQALKEG SWVLLDELNL
APQSVLEGLN AILDHRAQVF IPELGCTFEC PPTFRVFACQ NPSTQGGGRK GLPKSFLNRF
TKVYVDELVE DDYLFICRSL YPSVPSPLLS KLIALNRQLH DGTMLYRKFG HDGSPWEFNL
RDVIRSCQFM QEAIHDLEVE SFLNVLYIQR MRTATDRKEV LRIYKAIFDK TPSINPYPRV
QLNPAYLVVG TAAIKRNLNQ SNIASEQLKL LPEIRQNLEA VAHCVQNKWL CILVGPSSSG
KTSVIRILAQ LTGYPLNELN LSSATDSSDL LGCFEQYNAF RNFRLVMTRV EHLVDEYNSL
LLQSSQEALF SNRSGLVSRW LSYLNKIDSS LVENPLFFLN DSETLSTLEE VVEDLEQVLK
EGVLPVSWSK KYLEQISKTI LQLQTHEKKQ STKFEWVTGM LIKAIEKGEW VVLKNANLCN
PTVLDRINSL VEPCGSITIN ECGIVNGEPV TVVPHPNFRL FLSVNPKFGE VSRAMRNRGV
EVFMMGPHWQ LNEDGSNCEE LVLRGVERFL ALSGIPGYKL VTSMAKAHVH AWLNGQSFGV
RITYLELEQW VHLFQLLLMN GNQLLWSLQL SWEHIYLSSL GVTDGKEVVD FVRETYLSDV
ELSELDSFMG GDLYLPGGWP KPFNLRDLTW YSRETTVRQN CMYLEFLGAQ YASHQPKISD
NVKSRDRELA AGEPRIIYSI DSWTLKKVLF PKALIGSSCA PDAANFENDL ASKMLLFAAN
WTIEQATEED IQLYLAWFSW FGSRLQQHCP FLLCFLNTLK VEFEHPIWNH ISRCRKNLKF
LCRLDPDAVP IPMLSSKLID VAASNDQSKP YSKSLFESLN SVGVLRRSYQ QWLVESNDNH
TDVSTFTRFL DSLRVLEKKI LCEIVGAPSF SVLIQLYTEV IDNHSFFWSG LVSSSDEYLL
FSFWSLIKSI KKMHSFFPGE VQVVLEESKN INNIVLHGHP EKSMLWAYGG HPSLPVSAEL
FHKQQEFLQL CSTVWPLKSE SDEHGNDHLT KAIPFSGPEL CLLALEGLCI SSYIADEDDV
DYVAAVQLDE IYQTFLERLK LEKKRLEDKM GFSEIDNTEN ITASCCVFCP EIVTTGSGFS
SWVKTCFIAS SESCSLDVEL LAALQHLLVA RPTEHQDLVD IRKLLKPALE YSLSSTRPPQ
TLVAHQKLLW AIDAHASELG VDTKIAGFAL EIWYWWHSVL WKNSQIGLMN ISDTGNCQIL
SPSMLIQPVK TATVAQILEN VFSVKDYSVQ SMKLLSASRY LWKSSQPYQE MPGSLLSIAR
SLFQQIIYTH QKSFESETFV AIKSVFHAIE KKQNKMDGIQ NLISLIGSSS HNKLKSVTHS
FVGPLAKRLY SDSSSNALCP TFVEFYCNLG LAWLYLGGLR FHLLNSLDVI DPAMKITCKL
LKLEEKISSL ELNIKVRGEC GYLSGLLYSG NNDESSEHTL SKLKTEHKRL QRKVIFRSDP
KKYQDLRRAL DEFAGFLTRP ISLVNDIEVL DWNQVVEQVF NWQETAISFI DRMSSDYSEY
VDITQPIQVS VYEMKLGLSL FVSGALLGKL LNRFDIDMVD SVMETIYALM RFPRDSSIAS
TTYTECLPPL HLSHGANSRA KSLGLDVGLL HKLISVSSAE DSRKASELQL KVALYKNLHA
RVLQFVANTG LLDEASFELL DKIYVELARI WMEMKFQAKT KADNLPGLYK FRSRDFKIDS
VMEVDISALG KYFPNESFSE WQEYLADDDT KNVKDMTHID QDEENLEDDW DLIQEHLDSI
YSTHNELFGF CDLSEKSGRF CITDSRRLDS FTDSYELGVS MIKGLRGLFT SSLDAKLVPE
HLLRLCLENK KNFTSNYQSA SKYNFYKDLD GPELGKMVKF LTPLQQRINS LLQEREDHPG
LQKLSGVLQM LLAIPSSTPL AKALSGLQFL LCKVHKLQEE GCKLPISDLL EPIISLASSW
QKVEFERWPT LLDEVQDQYE LNARKLWLPL FSVLFQKDAV EISEHENESI SQSLVEFIET
SNVGEFRRRL QLLFCFLLQL SMGSSLGIYS SDSHKRRVEM CYNIFGFYIQ FLPVVMEQLD
LNRKNVETEL KEVLKLCRWE RPDNYLYNET TKRTRQKVKK LIQKFTDMLR LPVMLVKPDL
TKERAQFLPL LDPDLMDGAS DMRIEVLVSA LDAEQLRDRS SWYVVWWNKL KESVGRFHQE
MHYKTLLMGA EHQYSSPVYQ GDWKNLWSTV ARIGETIAGC SDLWRNSDRD VAKKRALFEL
LKLLESSGLQ KHKFENIEMS NHFKGLLYQP AYDPKHLLLL THTKSNIHPS MGVEDQNKEN
SLVEWRVANE FYFKSLASVQ LMLNIDRKHS DVTAEQVKRA ISFLNHLVEI QRQQRKSAYA
FAELFNRFRQ CVLSLARLLG DSVGADRKDD SVFSFPQNQH AVFNCLWLQK QLFDNITAML
LEESALLRTV GSTHLDSCQA VKTSSRSLLS FIEILIPIAQ NSKASLDRLL LDCNGFIITP
SSSLKQFVTQ HMVQVLRQNF DQLTDLENQI SSFCENNEKS YCRDVLLSQF SPVFKEGKLL
AENLNCLLNV RDQSTGMEPK ERLFLEENLA SIFANVKDVI GKLCSYKDGS LSQEEEMNIT
TWDGLFKKAE NDLNLDNLCK LLSESFGSIE QLLNSSGVLS AGVGDQLKQL QAFLDLLLSF
GDCYLKEFLA ISKTVSLITH VLASVLADLF TKGFGISKNE EDDDSKVDKS EAAEGTGMGD
GVGAKDVSDQ IEDEDQLHGT DKKEEEEKEQ DDVLGKNKGI EMSDEFDGKE YSVSEDEEED
KEDEGSEDEP LDNGIGDVGS DAEKADEKPW NKDEEDEEEN MNEKNESGPS IVDKDTRSRE
LRAKDDGVET ADEPEESNTS DKPEEGNDEN VEQDDFDDTD NLEEKIQTKE EALGGLTPDV
DNEQIDDDME MDKTEEVEKE DANQQEEPCS EDQKHPEEGE NDQEETQEPS EENMEAEAED
RCGSPQKEEP GNDLEQEPET EPIEGKEVMS EDMMKPNFRN DNISGVESGS QNPHGSNVLG
AGSTAPQENL SATDVTDELT DSMDLPSSSN TEMNLMMTNM ANGETLTDNL PKMEFPQNQS
STAQQTKVNP YRNVGDALKE WKERVRISSD LGEKQEAENE MEDPDASEYG FASQFDAGTS
QALGPALPEQ VNTDMREGES EEEKLAGNQD DVSPMDIDDL NPENKPAVQS KPSISNSIAE
QVQEPDTDRT HQENSPIHNF GDGNSRMDSM VSVDNTFLGE EACNLDRMQV TDNDSESNQD
NQEDPDARSN AVVLWRRCEL LTAKPSQELA EQLRLILEPT LASKLSGDYR TGKRINMKKV
IPYIASHYRK DKIWLRRTKP NKRDYQVVIA VDDSRSMSES GCGDFAIRAL ATVCRAMSQL
ELGSLAVASF GKQGSIKMLH DFGQSFTTES GIKMISNLTF KQENLIEDQP VVNLLRNMNE
MLENLASTRR QSYGSNPLQQ LVLIIGDGKF HEREKLKRTV RSFLQQKRMV VYLLLDDAEQ
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