MDN1_GIAIN
ID MDN1_GIAIN Reviewed; 4835 AA.
AC Q8T5T1;
DT 19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Midasin;
DE AltName: Full=Dynein-related AAA-ATPase MDN1;
DE AltName: Full=MIDAS-containing protein;
GN Name=MDN1;
OS Giardia intestinalis (Giardia lamblia).
OC Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX NCBI_TaxID=5741;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10930750; DOI=10.1111/j.1574-6968.2000.tb09242.x;
RA McArthur A.G., Morrison H.G., Nixon J.E., Passamaneck N.Q., Kim U.,
RA Hinkle G., Crocker M.K., Holder M.E., Farr R., Reich C.I., Olsen G.E.,
RA Aley S.B., Adam R.D., Gillin F.D., Sogin M.L.;
RT "The Giardia genome project database.";
RL FEMS Microbiol. Lett. 189:271-273(2000).
RN [2]
RP IDENTIFICATION, GENE NAME, AND SIMILARITY TO OTHER FAMILY MEMBERS.
RX PubMed=12102729; DOI=10.1186/1471-2164-3-18;
RA Garbarino J.E., Gibbons I.R.;
RT "Expression and genomic analysis of midasin, a novel and highly conserved
RT AAA protein distantly related to dynein.";
RL BMC Genomics 3:18-18(2002).
CC -!- FUNCTION: Nuclear chaperone required for maturation and nuclear export
CC of pre-60S ribosome subunits. Functions at successive maturation steps
CC to remove ribosomal factors at critical transition points, first
CC driving the exit of early pre-60S particles from the nucleolus and then
CC driving late pre-60S particles from the nucleus.
CC {ECO:0000250|UniProtKB:Q12019}.
CC -!- SUBUNIT: Associates with pre-60S ribosomes in the nucleoplasm.
CC {ECO:0000250|UniProtKB:Q12019}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q12019}. Nucleus, nucleoplasm
CC {ECO:0000250|UniProtKB:Q12019}.
CC -!- SIMILARITY: Belongs to the midasin family. {ECO:0000305}.
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DR EMBL; AF494287; AAM12656.1; -; Genomic_DNA.
DR SMR; Q8T5T1; -.
DR PRIDE; Q8T5T1; -.
DR VEuPathDB; GiardiaDB:DHA2_153310; -.
DR VEuPathDB; GiardiaDB:GL50581_3431; -.
DR VEuPathDB; GiardiaDB:GL50803_0039312; -.
DR eggNOG; KOG1808; Eukaryota.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 6.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR040848; AAA_lid_7.
DR InterPro; IPR011704; ATPase_dyneun-rel_AAA.
DR InterPro; IPR012099; Midasin.
DR InterPro; IPR041190; Midasin_AAA_lid_5.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR Pfam; PF07728; AAA_5; 5.
DR Pfam; PF17865; AAA_lid_5; 1.
DR Pfam; PF17867; AAA_lid_7; 1.
DR PIRSF; PIRSF010340; Midasin; 1.
DR SMART; SM00382; AAA; 5.
DR SUPFAM; SSF52540; SSF52540; 6.
DR SUPFAM; SSF53300; SSF53300; 1.
DR PROSITE; PS50234; VWFA; 1.
PE 3: Inferred from homology;
KW ATP-binding; Chaperone; Nucleotide-binding; Nucleus; Repeat.
FT CHAIN 1..4835
FT /note="Midasin"
FT /id="PRO_0000096335"
FT DOMAIN 4629..4818
FT /note="VWFA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT REGION 12..161
FT /note="AAA-ATPase protomer 1"
FT /evidence="ECO:0000255"
FT REGION 324..689
FT /note="AAA-ATPase protomer 2"
FT /evidence="ECO:0000255"
FT REGION 797..1049
FT /note="AAA-ATPase protomer 3"
FT /evidence="ECO:0000255"
FT REGION 1096..1375
FT /note="AAA-ATPase protomer 4"
FT /evidence="ECO:0000255"
FT REGION 1489..1738
FT /note="AAA-ATPase protomer 5"
FT /evidence="ECO:0000255"
FT REGION 1821..2110
FT /note="AAA-ATPase protomer 6"
FT /evidence="ECO:0000255"
FT REGION 2197..4058
FT /note="Linker"
FT /evidence="ECO:0000250|UniProtKB:Q12019"
FT REGION 4033..4056
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4108..4523
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4112..4126
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4156..4182
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4224..4242
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4253..4267
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4279..4293
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4300..4315
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4344..4364
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4382..4418
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4431..4469
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4479..4501
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 31..38
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00136"
FT BINDING 356..363
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 814..821
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 1127..1134
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 1513..1520
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 1839..1846
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 4835 AA; 539740 MW; 3A9E12417DB04A50 CRC64;
MSGYFTDLQN AEVLRAAESI VSSGQAVLLC GPSASGRTAL LSHLASKLGA KPPIPLHLTT
AQDTRDLIGS YVMTNKPGDF RFILGPLAYA AQSGRWISIE EITTISQDSL LLLSSVVNTR
TLSVGSYQIS VHPDFRIHAK TSADPALLST IVKSMFYPLV LPPLGNHLFQ VLTKHCNRGI
CTILAHIHVQ FETHRLTAGR ISASDMVKWA RRIDLDLKNG GAVLNKAVQE ASAGTAATTL
VSWLDDNTRM VMIKNAWEAF VLRYSKEQTR RAFLDILRLS LNIHEELMLS TLLKIKVDIR
HDYTQKRLVC GRVSLPFYES PDERSLDDIC RTNHTTRLLE IISSAIRNNE PLLLVGPTGI
GKTTCLQVIA RALGKKLHVV NMSSQTDAAD LLGGYVPATL DRILRNLYDA ITGSLGLYIS
VRKNQIFVTE LHKSYAEKDI AKFLANCETA LSGMRQVLRK EVGEVVEQGS TEQRVSHDKK
YARRGTYENS LYFLQSLGDT LEQATELHKI LCHEVETKKP TMAFKYQEGL LVQALVKGDW
ILIDEINLAS YDLLDVISQL VNPEHNEIAI PDKGFVAKNP HFRVFAAMNP GSDVGKKDLP
PTIRRCFTEI NVSEMSDDVD IVALTKSYLR MDDAMIEDRQ GLDPNVVYQL FTVLKARAKT
DLVTAAEAKS PCFSLRSLCR ALSFAHRFRA AAGLRRSMYE GFMLAFGSML NEKSRQAVHA
LILDKLLNGN KEYLLNPFII QPFNKPCTAM IFQATTKDEA VTVEYQEPQA AHGFLEEEKS
RARSTDYVLT KSTRSYMTTI SRAIAAQLPI LLEGTTSSGK TSLIKHIAKQ FGCPITRINN
HEHTDLSEYF GSYQPDPLTG QLVFKDGPLV TGMKQGHWVI LDELNMASSE ILEALNRLLD
DNRELLVPDT QEIVRPAPGF LLFATQNPSG SYAGRKMLSE AFQNRFIMIE FADISTEELK
EILINRSTSR HLAPQYCEKL ITTIQAIKMQ LSHRNNNAVM TNALITLRDL FRVADRLPRT
LTELAMGIFE LIGERQRDPA DKQLVAEIIA KNLGLKTFSV SAAEQEYAIR VRPIQSMIEK
ALKAGADSPK SAFLLKFQDI VWTPSMIRLF ALLFTSVSNG ESPLLVGVSG AGKTTSVELI
AAIMAQQLVQ VNLHKHTESA DFIGSLRPLR SRESMHAHLS VLKEYAATTT NVDKAVYAEI
KRLETTLGTK LFEWEDGPIL NCMKRGHILL LDEVSLADES VLERLNSVLE TSRELTVAEN
PNMPRPVIAH TGFKLIATMN PAGDYGKKEL SPAMRSRLTE FWMPHIRDIN EVRMILTRKL
QKTAIYRLGH QQGLDVVSLL ADFFAEMDRI TQSGRLGDVF TLSIRDILAV CDYITEVQTK
EGTELGQMLI DAVTLSILDG LPVRTQLGNM PACREVKHEM VLYLANRILS ANLTDIDLIQ
DLTVSISKDT NELTFLQAST QTVLATIPPG PQYNHAKALR KMTSFRLDAP TTIKNACRIA
KALRFQRPIL LEGDPGVGKS ALVSAIAEIC GYSLVRINLS EQTDLSDLLG SDLPAENGFR
WVDGVLLKAV KEGAFILLDE LNLANQTVLE GLNSLLDHRR SLFIPELMLS VKSPDTLRIF
GTQNPRSQGS GRKGLPQSFI NRFLNVYVDV LKSQDYDWIL SNLFTDIPTY VLDYILGSTK
QLRAGLEELK FGISGGPWEL NVRDMMRLCD MLTTTPGLAQ GITLAHAKHY AHILFGYRFR
TADDDAYVQN VLFSPHSLDS VAEATQSHHW YSLRPLWNVT EDVVKIGSLA IPRIDPVFRD
VLGTKLSYAH TSCFLTAQLV ALEALAVAVT QNLSCILVGS PESGKSSILR TLADVVRQPL
VHISASTATD VSDLIGAYEQ VDVLYDLKSV IRDLCLFVHS LPIKASYSAI SFSEFLKDLT
ASLDREIGDG LGSKSRAISM ESIVSEVVQK LLLEFMAKFS PDLDSGGLAA KNFSLFCSEI
LTQDTTPVDE RFSPHMPTIP ELCKSMASLY LSTQQSQTGR FVWRDSELIR ALERGYWVEL
TNANFCMPSV LDRLNSLLER GGGLEILEQG LEETRSIKVH PNFRLFISYA PTSDISRALR
NRSLELSIDI QVSHYNLLDI VRCVYTSSTA SGVSLDPYLI KLVSLVYFEL SRDNPEIVGL
GLLFKWLKLL VTYHKRFSTS VGDPKAVSSW VDRSFSSVFI ASTLNADLHR QYMQVYQNAR
LALQGDGSLE ACTLVEAAAV LNQLTLGAVR KLSISNSLQH SLALLELRTT VKPLDQLVSL
ADQEPQIMEP FSQVSGWLAI CVGHSLDFQH HKELVSNLLT STDINPMYLL TPPEPSSQAL
LKYISQGYLD MYSSPDSIIH QVIKYVSLCS PFYASILKLF IEGKFTTKDS YLLLYLIELS
QHLATDPNNY DYVALFFSLF ELLISHSIDP STKTYSLGQR QVMRTFFDMI LTDQYFESMP
RLLSGYIRSL FISAARNDLE TAWLAVRKNL ALARISVHYL PELFKVFRYV IERLNLCISP
EGVVFLQEGL SDSITASLWK DILATVIYMA CRADDQTGDY VPKLAILQDS ANRIDLCASK
LSGNQHPTAR ALAQMQSLFL LFTGKAFCMQ VSDTLEGWSQ RDLTTQLPIL LFRSMSFYHR
NAAGPITYLR GPIFVSTGEF VTSSLSVPAL LTNAIEEQAT SVLKSLLCRS EDFLSYVGNV
INSITTIVLR LFFAALEREA PSCDFTDGSF QLFALILETI VHSSNPLLAS LTQGVEDILK
AIISVQLSPS PLLLSGLVLT VASLVLYLLT SIANQRDPIV MTKHREVLYR RLAKLIATSI
EDSKLHALFV FGDGALSESS SIYKELSTLY NSYAEAADQY AAELLVRADD EDNTFFEDLS
SQLASLDGFE VHVLTFVSGV LNLEKVLQMH ESTECIEAIL DGLAVAERQI STTLAALLPR
LINYTVESSY YLDMTIPIAS FLMCLRVGLS AVISSVYAHV CDAINAARRL SSEGIGLQIT
LQDLLLLIQR VVVGTGDEFL DLLCYDKFIR SCCSSKTVST LHVARLFTLS LLQYSSCSKG
VQGDVSQFLL ADTLRPERVS SIAAQFEIEE ARSYRKYIAD HSIFKTRNQS STAEGVSEED
ELKTQIAALF PKNDSHFIKS DTSSDDEQPI SMSAQTKVDY DDAYATSFFT RMNMILIIGN
CILHLYTSDT EALPIQERLQ NLSSILQAHV PHLSRPLVAL QAVTYALSIC AGGVSSNLSI
RSPLTPETDR LMYPLVTGAT MGLVRVLSSR SPNVDYAKLQ TGKTRRAIHC EDIYSRPYPY
ETAEFCSTLL RKIIVRTQHL LTEWPDNVQL LDIMHVCDKL MELPVLEAPL VMFLTGAEVL
VRHLETWDKG APAVYKYVTN PNVPVPESLT KSLIHLMVTW RRVELMSWNG FFSRVHTQFS
NVSLTYWPEL LIASLTYQRS VTQGEPSTEA YYAQMREFLY ESNVGEFPVR LKLLYTLSAL
LSNIEELAEP ELVSSPHKTM TSAVLFSIAS EAVEFAKYMN KEFEEEAAQI KKRFEDQIRI
FEWNERSSYT SWLTAQNSHR LCTKIIREYT EVLQHATRAF HMGYSATYDR IPLSVTVQEY
LPLSSESGML PPALSQELVE RLNYCVEQMC HGRKESETLK RNEKNRLLSD VLDVLKETLG
CVLSSGFAFK QSFGWKLAHL CLPSVHSTFE LMHMFHDSLC QRCNEVEPHD IERMKRIIAC
LNQVFVQLSE LVVELADIEL LSRVMRTAPT GSSKSQADSV LYVYLADNSV PQLLTTQVMS
HVTKAHKMCM SLQEFLIGLP LDSCKSSPTK LHEQIGGFLE RFSADSVTAL NRIKIFSKFS
ALIKRLCSIT LIPLAFFEEA KAILQQILAD LSPIFSALIA VLPTAPISTF VTEHAKYLAQ
VTEVIDTLTE TAAQCRVEEA ATVSSIEWPY FALPFASVGE EGLSVEIIVD NAKKQVSAYM
TAFFTSTLQP ILQCATRHVV SLPVLPQKLL CQIETLKGDM QALADVLAKF IGPLISGAIA
LLRTGFHCHV DDDQKETDND NQSGLGLGDG TGDQNVSKEA AKELCEDDLM GNQNDRDQQE
QQEKDGDDEA IEMQNDFGGM SESLHHDIEE EDSNGSDEEE VLEKEMGDEQ GEAIDERNYN
EDDDSAEEYS DEQKKNTNNN NDTMELAAQE KDQDSINSEL SDPSGEQHEE QADATGSTDE
QAQEDDYNDL DDKNLSGQSD LSVPEADGED ETVNEELEEE QQQMSDLSNP DQDACAIEED
DDRDLPSSDE NAEEHDEHEA PVDIDDNEAS DEQSTYNDND RDDAINISAQ QQATNDEEEM
QKDTEYDQEN ITDSNPDANE VGTNDQKQTH EDNDQFRQEN IEDQWEAEST ENSQGEGAES
ADLKEGNPDM SLEEFQRIWK ERLNIHDRES EKDEAAEPQD MPLQSNKTVE FDDSKSGRDG
ALGLTESKHR NLTNQEFDNP NEERNVEHNS SCETSQSSHD RPPAEHLNPE ISDEGEESST
ASDKQEQAVL SHMRESSKDL LNPEGEVYQE LAVSLASEET KRAPEDVAAA SARGNHLLLD
LIKQTSAAAF SLAERLRIIL EPTVTSDLKG DFRTGKKLNL RRIIPFIASE FQKDKIWLRR
TKPSKRVYQV LLAVDDSSSM APIAKYALQA ITLLFNACKF LEVGQLSVFS FGQKFELLLP
ITDQYNDASL AYAIGSFTFA QNETRVSDAI SIASDYLDSV RFFKGSDSAL QLLLMISDGR
LKEKGGVARE IRKCMGRGQL PVLIVLDTDK KSIMDIKSVS FITDSSGKRV RQTSMYLDDF
PFQCYALLRH IEDLPETLVA VIKRWIESVS VYNAV
