ID   MDO2_ANAMC              Reviewed;          27 AA.
AC   P83447;
DT   01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-2002, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Macrodontain-2;
DE            EC=3.4.22.-;
DE   AltName: Full=Macrodontain II;
DE   Flags: Fragment;
OS   Ananas macrodontes (False pineapple) (Pseudananas macrodontes).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Bromeliaceae;
OC   Bromelioideae; Ananas.
OX   NCBI_TaxID=203992;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL
RP   PROPERTIES, SUBUNIT, AND MASS SPECTROMETRY.
RC   TISSUE=Fruit;
RX   PubMed=11517945; DOI=10.1515/bc.2001.108;
RA   Lopez L.M.I., Sequeiros C., Trejo S.A., Pardo M.F., Caffini N.O.,
RA   Natalucci C.L.;
RT   "Comparison of two cysteine endopeptidases from Pseudananas macrodontes
RT   (Morr.) Harms (Bromeliaceae).";
RL   Biol. Chem. 382:875-878(2001).
CC   -!- FUNCTION: Cysteine protease that shows preferential cleavage as
CC       follows: Ala-|-Xaa > Gln-|-Xaa > Tyr-Xaa. Hydrolyzes the synthetic
CC       peptide substrate Bz-Phe-Val-Arg-pNA. {ECO:0000269|PubMed:11517945}.
CC   -!- ACTIVITY REGULATION: Inhibited by the general cysteine protease
CC       inhibitor E-64 (L-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane).
CC       {ECO:0000269|PubMed:11517945}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=8.9 uM for Bz-Phe-Val-Arg-pNA {ECO:0000269|PubMed:11517945};
CC       pH dependence:
CC         Optimum pH is 7.5-8.5. {ECO:0000269|PubMed:11517945};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:11517945}.
CC   -!- MASS SPECTROMETRY: Mass=23703; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:11517945};
CC   -!- SIMILARITY: Belongs to the peptidase C1 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU10088, ECO:0000255|PROSITE-ProRule:PRU10089,
CC       ECO:0000255|PROSITE-ProRule:PRU10090}.
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DR   AlphaFoldDB; P83447; -.
DR   MEROPS; C01.161; -.
DR   GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR000668; Peptidase_C1A_C.
DR   Pfam; PF00112; Peptidase_C1; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Protease; Thiol protease.
FT   CHAIN           1..>27
FT                   /note="Macrodontain-2"
FT                   /id="PRO_0000050562"
FT   NON_TER         27
SQ   SEQUENCE   27 AA;  3037 MW;  118D1932988BCF98 CRC64;
     AVPQSIDWRD YGAVNEVKNQ NPCGSCW