MDPK_EMENI
ID MDPK_EMENI Reviewed; 265 AA.
AC C8VQ62; Q5BH28;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 03-NOV-2009, sequence version 1.
DT 25-MAY-2022, entry version 63.
DE RecName: Full=Oxidoreductase mdpK {ECO:0000303|PubMed:21351751};
DE EC=1.-.-.- {ECO:0000305|PubMed:22730213};
DE AltName: Full=Monodictyphenone synthesis protein k {ECO:0000303|PubMed:20139316};
GN Name=mdpK {ECO:0000303|PubMed:20139316}; ORFNames=ANIA_10044;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX PubMed=20139316; DOI=10.1128/aem.02187-09;
RA Chiang Y.M., Szewczyk E., Davidson A.D., Entwistle R., Keller N.P.,
RA Wang C.C., Oakley B.R.;
RT "Characterization of the Aspergillus nidulans monodictyphenone gene
RT cluster.";
RL Appl. Environ. Microbiol. 76:2067-2074(2010).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX PubMed=21351751; DOI=10.1021/ja1096682;
RA Sanchez J.F., Entwistle R., Hung J.H., Yaegashi J., Jain S., Chiang Y.M.,
RA Wang C.C., Oakley B.R.;
RT "Genome-based deletion analysis reveals the prenyl xanthone biosynthesis
RT pathway in Aspergillus nidulans.";
RL J. Am. Chem. Soc. 133:4010-4017(2011).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX PubMed=22730213; DOI=10.1002/cbic.201200014;
RA Simpson T.J.;
RT "Genetic and biosynthetic studies of the fungal prenylated xanthone
RT shamixanthone and related metabolites in Aspergillus spp. revisited.";
RL ChemBioChem 13:1680-1688(2012).
CC -!- FUNCTION: Oxidoreductase; part of the gene cluster that mediates the
CC biosynthesis of monodictyphenone, a prenyl xanthone derivative
CC (PubMed:20139316, PubMed:21351751, PubMed:22730213). The pathway begins
CC with the synthesis of atrochrysone thioester by the polyketide synthase
CC (PKS) mdpG (PubMed:20139316). The atrochrysone carboxyl ACP
CC thioesterase mdpF then breaks the thioester bond and releases the
CC atrochrysone carboxylic acid from mdpG (PubMed:20139316). The
CC atrochrysone carboxylic acid is then converted to atrochrysone which is
CC further transformed into emodin anthrone (PubMed:20139316). The next
CC step is performed by the anthrone oxygenase mdpH that catalyzes the
CC oxidation of emodinanthrone to emodin (By similarity). Emodin is
CC further modified to yield monodictyphenone via several steps involving
CC mdpB, mdpC mdpJ, mdpK and mdpL (PubMed:20139316, PubMed:21351751).
CC These enzymes with xptA, xptB and xptC are also proposed to be involved
CC in the synthesis of shamixanthone from emodin (PubMed:22730213).
CC Especially, direct reduction of emodin by the short chain dehydrogenase
CC mdpC followed by dehydration catalyzed by the scytalone dehydratase-
CC like protein mdpB gives loss of oxygen and formation of chrysophanol
CC intermediate in two simple steps (PubMed:22730213).
CC {ECO:0000250|UniProtKB:Q0CCY3, ECO:0000269|PubMed:20139316,
CC ECO:0000269|PubMed:21351751, ECO:0000269|PubMed:22730213}.
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000269|PubMed:20139316, ECO:0000269|PubMed:21351751,
CC ECO:0000269|PubMed:22730213}.
CC -!- DISRUPTION PHENOTYPE: Impairs the production of monodictyphenone, but
CC still enables the synthesis of intermediates until emodin
CC (PubMed:20139316, PubMed:21351751). Results in major accumulation of
CC chrysophanol and its 6-hydroxymethyl shunt product aloe-emodin
CC (PubMed:22730213). {ECO:0000269|PubMed:20139316,
CC ECO:0000269|PubMed:21351751, ECO:0000269|PubMed:22730213}.
CC -!- SIMILARITY: Belongs to the avfA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAA66025.1; Type=Erroneous gene model prediction; Note=The predicted gene AN0152 has been split into 2 genes: ANIA_10044 and ANIA_10023.; Evidence={ECO:0000305};
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DR EMBL; BN001308; CBF90090.1; -; Genomic_DNA.
DR EMBL; AACD01000005; EAA66025.1; ALT_SEQ; Genomic_DNA.
DR AlphaFoldDB; C8VQ62; -.
DR SMR; C8VQ62; -.
DR STRING; 162425.CADANIAP00002589; -.
DR EnsemblFungi; CBF90090; CBF90090; ANIA_10044.
DR EnsemblFungi; EAA66025; EAA66025; AN0152.2.
DR VEuPathDB; FungiDB:AN10044; -.
DR eggNOG; ENOG502SM0C; Eukaryota.
DR HOGENOM; CLU_090039_1_0_1; -.
DR InParanoid; C8VQ62; -.
DR OMA; AYCRNKA; -.
DR OrthoDB; 1166292at2759; -.
DR Proteomes; UP000000560; Chromosome VIII.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:1900815; P:monodictyphenone biosynthetic process; IMP:AspGD.
DR GO; GO:0044550; P:secondary metabolite biosynthetic process; IMP:AspGD.
DR GO; GO:2001307; P:xanthone-containing compound biosynthetic process; IMP:AspGD.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF13460; NAD_binding_10; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 3: Inferred from homology;
KW Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..265
FT /note="Oxidoreductase mdpK"
FT /id="PRO_0000437063"
SQ SEQUENCE 265 AA; 28877 MW; 8DFE9CA6D8B37725 CRC64;
MPLATYAVLG ATGNTGTALI QNLLSPPSSE MHINAYCRNK PKLLNLLPEL NDTKNVTIFE
GSITDLSLIT ACIRNTRAVF LTVTSNDNIP GCRLSQDSVQ TVLEALKQIR TAEPNAVVPK
LVLLSSATID PHLSRKMPSW FLPIMKTAAS NVYADLIKAE EMLRANESWV TSIFIKPAGL
SVDIQRGHKL DFDEQESFIS YLDLAAAMLE AANDTDGRYD GRNVSVVNTG GKARFPPGTP
KCIIVGLLRH FFPGLHRFLP TTGPS
