MDRP_PLAMR
ID MDRP_PLAMR Reviewed; 424 AA.
AC A0A1C7E424;
DT 13-FEB-2019, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2016, sequence version 1.
DT 25-MAY-2022, entry version 26.
DE RecName: Full=Na(+), Li(+), K(+)/H(+) antiporter {ECO:0000305};
DE AltName: Full=Major facilitator superfamily transporter MdrP {ECO:0000305};
DE Short=MFS transporter MdrP {ECO:0000305};
DE AltName: Full=Multidrug efflux pump MdrP {ECO:0000305};
DE AltName: Full=Na(+) (Li(+)/K(+))/H(+) antiporter {ECO:0000303|PubMed:30061877};
GN Name=mdrP {ECO:0000303|PubMed:30061877};
GN ORFNames=BBI11_14530 {ECO:0000312|EMBL:ANU18183.1};
OS Planococcus maritimus.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Planococcaceae; Planococcus.
OX NCBI_TaxID=192421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17275 / CIP 108219 / JCM 11543 / TF-9;
RA See-Too W.S.;
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP SUBCELLULAR LOCATION.
RC STRAIN=DSM 17275 / CIP 108219 / JCM 11543 / TF-9;
RX PubMed=30061877; DOI=10.3389/fmicb.2018.01601;
RA Abdel-Motaal H., Meng L., Zhang Z., Abdelazez A.H., Shao L., Xu T.,
RA Meng F., Abozaed S., Zhang R., Jiang J.;
RT "An uncharacterized major facilitator superfamily transporter from
RT Planococcus maritimus exhibits dual functions as a Na+(Li+, K+)/H+
RT antiporter and a multidrug efflux pump.";
RL Front. Microbiol. 9:1601-1601(2018).
CC -!- FUNCTION: Exhibits dual functions as a Na(+)(Li(+)/K(+))/H(+)
CC antiporter and a multidrug efflux pump. Catalyzes the efflux of Na(+),
CC Li(+) and K(+) in exchange for external protons. Shows a preference for
CC Na(+), followed by K(+) and Li(+). Can also function as a multidrug
CC efflux pump. Transports ethidium bromide and norfloxacin.
CC {ECO:0000269|PubMed:30061877}.
CC -!- ACTIVITY REGULATION: Norfloxacin transport is inhibited by CCCP.
CC {ECO:0000269|PubMed:30061877}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 9.0 for Na(+)/H(+) and Li(+)/H(+) antiport activities.
CC Optimum pH is 8.5 for K(+)/H(+) antiport activity.
CC {ECO:0000269|PubMed:30061877};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:30061877};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; CP016538; ANU18183.1; -; Genomic_DNA.
DR RefSeq; WP_068464567.1; NZ_CP016538.2.
DR AlphaFoldDB; A0A1C7E424; -.
DR SMR; A0A1C7E424; -.
DR EnsemblBacteria; ANU18183; ANU18183; BBI11_14530.
DR KEGG; pmat:BBI11_14530; -.
DR OrthoDB; 1452500at2; -.
DR Proteomes; UP000092748; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF07690; MFS_1; 2.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; Antiport; Cell membrane; Ion transport; Membrane;
KW Potassium; Potassium transport; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..424
FT /note="Na(+), Li(+), K(+)/H(+) antiporter"
FT /id="PRO_0000446277"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 367..389
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..415
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 424 AA; 47923 MW; CE475B2D38EBC7C8 CRC64;
MKIKDWNRSL KVRLVGEFFM NTSFWMVFPF LAIYFAEEFG KGLAGMLLII SQIFSVAANL
FGGYFADRFG RKRMLVSAAV AQGFAFLLFA LANSPWLTSP ELSFVAFTLA GMCGSLYWPA
SQAMIADVIP EKYRSDVFAV FYTTLNIAVV IGPLFGAVLF FSYRFELLLT VAIISVLLGL
LLRFYTEETL SAEVLEKWKE GNATGWRGAL LTQVKDYGII LKDRVFLLFV IAGILGAQTF
MQLDLVIPVY LKETIDRQTI LDFLGREWSV TGETSFGILL AENGLIVALL TVVITRWMTK
FPEKWVFFFS ALLFGLSMAI FPMTSWFWIF FVAMAVFTFA ELMVVGLQQS FISKLAPESM
RGQYFAAASL RYTIGRMIAP ISIPMTAWFG FGWTFIILGS FAVLSGFVYL WMFHLYDKRT
VANI
