ID   MDS1_PHYTB              Reviewed;          18 AA.
AC   C0HLE1;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   10-OCT-2018, sequence version 1.
DT   25-MAY-2022, entry version 7.
DE   RecName: Full=Medusin-TR1 {ECO:0000305};
DE            Short=MDS-TR1 {ECO:0000305};
DE   AltName: Full=Phylloseptin-4TR {ECO:0000303|PubMed:29980138};
DE            Short=PLS-4TR {ECO:0000305};
OS   Phyllomedusa trinitatis (Trinidad leaf frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Phyllomedusa.
OX   NCBI_TaxID=332092;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SYNTHESIS, SUBCELLULAR LOCATION, MASS
RP   SPECTROMETRY, AND AMIDATION AT LEU-18.
RC   TISSUE=Skin secretion;
RX   PubMed=29980138; DOI=10.1016/j.cbd.2018.06.006;
RA   Mechkarska M., Coquet L., Leprince J., Auguste R.J., Jouenne T.,
RA   Mangoni M.L., Conlon J.M.;
RT   "Peptidomic analysis of the host-defense peptides in skin secretions of the
RT   Trinidadian leaf frog Phyllomedusa trinitatis (Phyllomedusidae).";
RL   Comp. Biochem. Physiol. 28:72-79(2018).
RN   [2]
RP   FUNCTION, AND SYNTHESIS.
RX   PubMed=30734396; DOI=10.1002/psc.3153;
RA   Pantic J., Guilhaudis L., Musale V., Attoub S., Lukic M.L., Mechkarska M.,
RA   Conlon J.M.;
RT   "Immunomodulatory, insulinotropic, and cytotoxic activities of
RT   phylloseptins and plasticin-TR from the Trinidanian leaf frog Phyllomedusa
RT   trinitatis.";
RL   J. Pept. Sci. 25:E3153-E3153(2019).
CC   -!- FUNCTION: Antimicrobial peptide with activity against Gram-positive
CC       bacteria S.epidermidis ATCC 12228 (MIC=50 uM) and S.aureus (MIC=64
CC       ug/ml and MBC=128 ug/ml) (By similarity) (PubMed:29980138). Not active
CC       against some Gram-positive bacteria (methicillin-resistant S.aureus
CC       (MRSA), E.faecalis), Gram-negative bacterium E.coli ATCC 25922 and
CC       fungus C.albicans at concentrations up to 100 uM (By similarity)
CC       (PubMed:29980138). Can only slightly inhibit the formation of biofilm
CC       by S.aureus (minimal biofilm inhibitionconcentration MBIC=512 ug/ml,
CC       minimal biofilm eradication concentration MBEC>512 ug/ml) (By
CC       similarity). Has an anti-inflammatory effect, since it inhibits the
CC       production of the pro-inflammatory cytokines TNF-alpha and IL-1beta
CC       (PubMed:30734396). Has high activity of stimulation of insulin release,
CC       which may protect the species from being eaten by predators by causing
CC       fatal hypoglycemia (PubMed:30734396). Is not cytotoxic to cancer line
CC       cells (PubMed:30734396). Shows very low hemolysis on horse erythrocytes
CC       and moderate hemolysis on mouse erythrocytes (By similarity)
CC       (PubMed:29980138, PubMed:30734396). {ECO:0000250|UniProtKB:A0A1M4BLT1,
CC       ECO:0000269|PubMed:29980138, ECO:0000269|PubMed:30734396}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:29980138}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:29980138}.
CC   -!- MASS SPECTROMETRY: Mass=1810.1; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:29980138};
CC   -!- MISCELLANEOUS: The primary structure of this peptide is identical to
CC       that of Medusin-PT (AC A0A1M4BLT1). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Medusin subfamily. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=The antimicrobial peptide database;
CC       URL="https://wangapd3.com/database/query_output.php?ID=02995";
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DR   AlphaFoldDB; C0HLE1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial; Cytolysis;
KW   Direct protein sequencing; Hemolysis; Immunity; Innate immunity; Secreted.
FT   PEPTIDE         1..18
FT                   /note="Medusin-TR1"
FT                   /evidence="ECO:0000269|PubMed:29980138"
FT                   /id="PRO_0000445201"
FT   MOD_RES         18
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:29980138"
SQ   SEQUENCE   18 AA;  1811 MW;  613872B0E68FC233 CRC64;
     LLGMIPVAIT AISALSKL