ID   MDS3_CANAL              Reviewed;        1383 AA.
AC   Q5ADT1; A0A1D8PKQ3;
DT   01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2017, sequence version 2.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Negative regulator of sporulation MDS3;
GN   Name=MDS3; OrderedLocusNames=CAALFM_C307320WA;
GN   ORFNames=CaO19.14052/14051, CaO19.6760/6759;
OS   Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=237561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA   Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA   Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA   Scherer S.;
RT   "The diploid genome sequence of Candida albicans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA   van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA   Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA   Chibana H., Nantel A., Magee P.T.;
RT   "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT   on the eight chromosomes.";
RL   Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA   Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT   "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT   specific measurements and provides a simple model for repeat and indel
RT   structure.";
RL   Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN   [4]
RP   FUNCTION.
RX   PubMed=12524333; DOI=10.1093/genetics/162.4.1573;
RA   Davis D.A., Bruno V.M., Loza L., Filler S.G., Mitchell A.P.;
RT   "Candida albicans Mds3p, a conserved regulator of pH responses and
RT   virulence identified through insertional mutagenesis.";
RL   Genetics 162:1573-1581(2002).
RN   [5]
RP   FUNCTION.
RX   PubMed=14663094; DOI=10.1099/mic.0.26640-0;
RA   Nobile C.J., Bruno V.M., Richard M.L., Davis D.A., Mitchell A.P.;
RT   "Genetic control of chlamydospore formation in Candida albicans.";
RL   Microbiology 149:3629-3637(2003).
RN   [6]
RP   FUNCTION.
RX   PubMed=16087754; DOI=10.1128/ec.4.8.1493-1502.2005;
RA   Richard M.L., Nobile C.J., Bruno V.M., Mitchell A.P.;
RT   "Candida albicans biofilm-defective mutants.";
RL   Eukaryot. Cell 4:1493-1502(2005).
RN   [7]
RP   DISRUPTION PHENOTYPE, AND VIRULENCE.
RX   PubMed=17241762; DOI=10.1016/j.micpath.2006.11.005;
RA   Jackson B.E., Wilhelmus K.R., Mitchell B.M.;
RT   "Genetically regulated filamentation contributes to Candida albicans
RT   virulence during corneal infection.";
RL   Microb. Pathog. 42:88-93(2007).
RN   [8]
RP   FUNCTION, AND INTERACTION WITH SIT4.
RX   PubMed=20457806; DOI=10.1128/mcb.01540-09;
RA   Zacchi L.F., Gomez-Raja J., Davis D.A.;
RT   "Mds3 regulates morphogenesis in Candida albicans through the TOR
RT   pathway.";
RL   Mol. Cell. Biol. 30:3695-3710(2010).
CC   -!- FUNCTION: Negatively regulates early sporulation-specific genes (By
CC       similarity). TOR signaling pathway component that contributes to
CC       morphogenesis as a regulator of this key morphogenetic pathway.
CC       Required for growth and hyphal formation at pH 9, for full virulence in
CC       a mouse model of systemic infection and for biofilm formation. Involved
CC       in chlamydospore formation, distinctive morphological feature of the
CC       fungal pathogen C.albicans that can be induced to form in oxygen-
CC       limited environments and has been reported in clinical specimens.
CC       {ECO:0000250, ECO:0000269|PubMed:12524333, ECO:0000269|PubMed:14663094,
CC       ECO:0000269|PubMed:16087754, ECO:0000269|PubMed:20457806}.
CC   -!- SUBUNIT: Interacts with SIT4. {ECO:0000269|PubMed:20457806}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Results in attenuated keratomycosis.
CC       {ECO:0000269|PubMed:17241762}.
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DR   EMBL; CP017625; AOW28734.1; -; Genomic_DNA.
DR   RefSeq; XP_719857.2; XM_714764.2.
DR   AlphaFoldDB; Q5ADT1; -.
DR   STRING; 237561.Q5ADT1; -.
DR   GeneID; 3638464; -.
DR   KEGG; cal:CAALFM_C307320WA; -.
DR   CGD; CAL0000193099; MDS3.
DR   VEuPathDB; FungiDB:C3_07320W_A; -.
DR   eggNOG; ENOG502QSQ9; Eukaryota.
DR   HOGENOM; CLU_401130_0_0_1; -.
DR   InParanoid; Q5ADT1; -.
DR   OMA; SGGMVFR; -.
DR   OrthoDB; 342740at2759; -.
DR   PRO; PR:Q5ADT1; -.
DR   Proteomes; UP000000559; Chromosome 3.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0045454; P:cell redox homeostasis; IBA:GO_Central.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 2.120.10.80; -; 1.
DR   InterPro; IPR015915; Kelch-typ_b-propeller.
DR   SUPFAM; SSF117281; SSF117281; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Kelch repeat; Meiosis; Reference proteome; Repeat; Sporulation;
KW   Virulence.
FT   CHAIN           1..1383
FT                   /note="Negative regulator of sporulation MDS3"
FT                   /id="PRO_0000422105"
FT   REPEAT          124..179
FT                   /note="Kelch 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          199..246
FT                   /note="Kelch 2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          356..402
FT                   /note="Kelch 3"
FT                   /evidence="ECO:0000255"
FT   REGION          450..506
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          625..644
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          653..825
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          858..884
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1063..1114
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1251..1289
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1321..1369
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        461..475
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        476..490
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        657..672
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        723..744
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        768..824
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1077..1114
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1337..1369
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1383 AA;  153707 MW;  298BB7B5DA7F73A6 CRC64;
     MSTLIPTASA CYSLQLPPTE KDDRLNLNVR TGSASTLYNS LIFVHGGLTI GLELLNYTIP
     ELNEIFYNRI NISASKYKTV EKYLSGELFY LSLIERNWSR VVLEELEIRP KPRLLHQICA
     FNNCLYLFGG LALSQENDED PTLVPCNDLW EFDLVLKKWT LLDDGSNYEL DDAVPSPRFN
     HKLTVISSLS FANRKDHFGL FIAGGKDKQS NEIYDNSIFD LVEKRYVGSQ PFRLVATTGD
     DTKDKETGLN EFVSNPEHFL NVDQTRNAIL SITDDADTPN RKQNKNPSNH HESIVVYGPT
     RQTGDCQNSL VSFKVGKREI KGGKVLRLHK NSRVAKLKQS IIPYNLRYPT AGLFGQNVVL
     TGFLPDEYEI SIFVYNRPTG KWSRLNIFCS HEYGSHRFWG GFAWQSHHKV ILIGNSMTSR
     TTSSVRFFSI MLTVSLPITN ILVSSELSKG RSNNRTSSFV SHGEQRHHRN KSQNDSLLKA
     ELSLESTTED STDSSSDKTA ESVLDAPVPL ANSRRQSFSS LGSDKSPTAV SFSDYVHYAA
     PKTTYTTIRS VFPPEAITLG RNAFNRIGDL IADIELVSCN GDRIPVSSAV LMERWGQHFI
     SLLAKGYINA VDKFETDQAL GLDENQRLRS KSSNSESSSS DIPKLKLSLS ESLLSSSSGE
     HDKKEKMGLS SAHKPQKDVP QFRLPFQDSS ESVNREEGSD CSKDRKTGGS SVSTAIDPHH
     VMPRKNSTSS FQSNSSSLLT SHLQDIPPQL PLPDEQIPAV PAAPVSYRSA SRKNSQDHSS
     PRSSLIHTLT VLRNIPVSKS PRESPFSSPR PSMSGPSGGS ADLFSSPFPS LKANYGKPPS
     NLRKKSYDMN DGTIESSLDS FSSGKSSMAK VSSVPGENPD SDESSIGFQE FNKNPISMFD
     NALLNFDNID SENFRMEPSL IPRKLYIPFM TLTVKAFCEY LYTGQIGNKW LLAPTLMDNL
     LISKFYRVPL LYDLISEILF GVIGKKEAYI IGEARKLKAR YFKLLRGANI PIDSNYEFPM
     DEYDGFLDTV DDGYLDITLL KKASKIHADS VAMSLKKKSV TSNNSRNASI RKEGSTTAES
     VLEEGEKQEE IVSKDDEDES RTGSTSEEDE VDKEYGLVYL EAKDKSLPTI GPRSKSIFDR
     QGLAAIEKLE EEQNDHQVHS IVEGEDHENL ITLDELVASD APSDYAIDLI FETATLVTDM
     KMLLRTSNLK AMTAKFRKCK ADIEKEMADI EYQLTQMPSG SRNLAMKSAN QLKESQLQSK
     SSPIIPTVST VTPSPLPSIS GVPSPRLPQQ QLPEAQLPKL TKSTSSLSRI ASHTSFKALR
     SMTDVTPMEK STSDKSFRGK LQQTMLSRTP TNRNEDQDSS SASSIKSSSK KRGIFGLLTG
     LKR