MDT28_CAEEL
ID MDT28_CAEEL Reviewed; 202 AA.
AC H9G301; O18692;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2012, sequence version 1.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Coiled-coil domain-containing protein mdt-28 {ECO:0000305};
GN Name=mdt-28 {ECO:0000303|PubMed:28603670, ECO:0000312|WormBase:F28F8.5b};
GN ORFNames=F28F8.5 {ECO:0000312|WormBase:F28F8.5b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, INTERACTION WITH MDT-6 AND MDT-30, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=28603670; DOI=10.7717/peerj.3390;
RA Kostrouchova M., Kostrouch D., Chughtai A.A., Kassak F., Novotny J.P.,
RA Kostrouchova V., Benda A., Krause M.W., Saudek V., Kostrouchova M.,
RA Kostrouch Z.;
RT "The nematode homologue of Mediator complex subunit 28, F28F8.5, is a
RT critical regulator of C. elegans development.";
RL PeerJ 5:E3390-E3390(2017).
CC -!- FUNCTION: Plays a role in normal growth and development.
CC {ECO:0000269|PubMed:28603670}.
CC -!- SUBUNIT: Interacts with mdt-6 and mdt-30.
CC {ECO:0000269|PubMed:28603670}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:28603670}. Cytoplasm
CC {ECO:0000269|PubMed:28603670}. Note=Mainly localizes to the nucleus.
CC {ECO:0000269|PubMed:28603670}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b {ECO:0000312|WormBase:F28F8.5b};
CC IsoId=H9G301-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:F28F8.5a};
CC IsoId=H9G301-2; Sequence=VSP_059205;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed in tissues including
CC epidermal, intestinal, pharyngeal and uterine, and is also expressed in
CC vulval muscle cells and gut granules. {ECO:0000269|PubMed:28603670}.
CC -!- DEVELOPMENTAL STAGE: Expressed from embryos to adults. Expressed in
CC embryos from the two-fold stage, and is ubiquitously expressed during
CC embryonic and larval stages of development.
CC {ECO:0000269|PubMed:28603670}.
CC -!- DISRUPTION PHENOTYPE: Viable, but sterile with defective development
CC that is prominent during the late larval stages of development. The
CC developmental defects include a squat body stature referred to as a
CC dumpy phenotype, irregular gut, defective gonadal growth, with
CC incomplete gonad development in some animals, a protruding vulva
CC phenotype, defective uterus and spermatheca formation, and excretory
CC canal defects. RNAi-mediated knockdown results in 44% of animals
CC arresting at either the embryonic or larval stage of development, and
CC in a range of phenotypes including defective molting, protruding vulvae
CC that burst, male tail ray defects and uncoordinated movement.
CC {ECO:0000269|PubMed:28603670}.
CC -!- CAUTION: Described as a member of the Mediator complex and a probable
CC MED28 ortholog but shows very low similarity to known MED28 proteins
CC and lacks the Pfam Med28 signature found in known MED28 proteins.
CC {ECO:0000305}.
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DR EMBL; BX284605; CAB03011.1; -; Genomic_DNA.
DR EMBL; BX284605; CCG28159.1; -; Genomic_DNA.
DR PIR; T21506; T21506.
DR RefSeq; NP_001256673.1; NM_001269744.1. [H9G301-2]
DR RefSeq; NP_001256674.1; NM_001269745.1.
DR AlphaFoldDB; H9G301; -.
DR SMR; H9G301; -.
DR IntAct; H9G301; 1.
DR STRING; 6239.F28F8.5b; -.
DR EnsemblMetazoa; F28F8.5a.1; F28F8.5a.1; WBGene00009223. [H9G301-2]
DR EnsemblMetazoa; F28F8.5b.1; F28F8.5b.1; WBGene00009223. [H9G301-1]
DR GeneID; 180048; -.
DR KEGG; cel:CELE_F28F8.5; -.
DR UCSC; F28F8.5; c. elegans.
DR CTD; 180048; -.
DR WormBase; F28F8.5a; CE09759; WBGene00009223; mdt-28. [H9G301-2]
DR WormBase; F28F8.5b; CE47319; WBGene00009223; mdt-28. [H9G301-1]
DR eggNOG; ENOG502SD7Z; Eukaryota.
DR InParanoid; H9G301; -.
DR OMA; IDSLMMH; -.
DR OrthoDB; 1576633at2759; -.
DR PRO; PR:H9G301; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00009223; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..202
FT /note="Coiled-coil domain-containing protein mdt-28"
FT /evidence="ECO:0000305"
FT /id="PRO_0000442207"
FT REGION 1..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 159..184
FT /evidence="ECO:0000255"
FT COMPBIAS 1..15
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 57..73
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 20..21
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_059205"
SQ SEQUENCE 202 AA; 22501 MW; AB7A0DF927D70158 CRC64;
MFEELDAEDG GEEQEMPMNV PEFGGGGEDI DDILGDAPDL PDDEYSNSVP QGAADGSIEN
DERRMRNDGA GSTNEDDEEP IEPTYLGDAI DSLMMHWCQL LTNVSVKAPV PPPSTLNHVK
EVAEVCSKHF RDASVDVNNE FTRLGVQWEM EQPYSQYAIE EENLDEAIER QETIIAAARE
MLNSRIQIYN EAHPNAGKHF TT
