MDTA_ECOLI
ID MDTA_ECOLI Reviewed; 415 AA.
AC P76397;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Multidrug resistance protein MdtA;
DE AltName: Full=Multidrug transporter MdtA;
DE Flags: Precursor;
GN Name=mdtA; Synonyms=yegM; OrderedLocusNames=b2074, JW5338;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RC STRAIN=K12;
RX PubMed=12107133; DOI=10.1128/jb.184.15.4161-4167.2002;
RA Nagakubo S., Nishino K., Hirata T., Yamaguchi A.;
RT "The putative response regulator BaeR stimulates multidrug resistance of
RT Escherichia coli via a novel multidrug exporter system, MdtABC.";
RL J. Bacteriol. 184:4161-4167(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION, AND INDUCTION.
RX PubMed=12107134; DOI=10.1128/jb.184.15.4168-4176.2002;
RA Baranova N., Nikaido H.;
RT "The baeSR two-component regulatory system activates transcription of the
RT yegMNOB (mdtABCD) transporter gene cluster in Escherichia coli and
RT increases its resistance to novobiocin and deoxycholate.";
RL J. Bacteriol. 184:4168-4176(2002).
CC -!- FUNCTION: The MdtABC tripartite complex confers resistance against
CC novobiocin and deoxycholate. MdtABC requires TolC for its function.
CC {ECO:0000269|PubMed:12107133, ECO:0000269|PubMed:12107134}.
CC -!- SUBUNIT: Part of a tripartite efflux system composed of MdtA, MdtB and
CC MdtC.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Peripheral
CC membrane protein {ECO:0000305}.
CC -!- INDUCTION: The mdtABC operon is transcriptionally activated by BaeR.
CC {ECO:0000269|PubMed:12107133, ECO:0000269|PubMed:12107134}.
CC -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC family. {ECO:0000305}.
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DR EMBL; AB089187; BAC06607.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75135.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA15928.2; -; Genomic_DNA.
DR PIR; A64974; A64974.
DR RefSeq; NP_416578.2; NC_000913.3.
DR RefSeq; WP_000678989.1; NZ_LN832404.1.
DR AlphaFoldDB; P76397; -.
DR SMR; P76397; -.
DR BioGRID; 4260423; 142.
DR ComplexPortal; CPX-2119; MdtABC-TolC multidrug efflux transport complex.
DR STRING; 511145.b2074; -.
DR TCDB; 8.A.1.6.2; the membrane fusion protein (mfp) family.
DR jPOST; P76397; -.
DR PaxDb; P76397; -.
DR PRIDE; P76397; -.
DR EnsemblBacteria; AAC75135; AAC75135; b2074.
DR EnsemblBacteria; BAA15928; BAA15928; BAA15928.
DR GeneID; 946604; -.
DR KEGG; ecj:JW5338; -.
DR KEGG; eco:b2074; -.
DR PATRIC; fig|511145.12.peg.2151; -.
DR EchoBASE; EB3809; -.
DR eggNOG; COG0845; Bacteria.
DR HOGENOM; CLU_018816_2_0_6; -.
DR InParanoid; P76397; -.
DR OMA; GQLMAIH; -.
DR PhylomeDB; P76397; -.
DR BioCyc; EcoCyc:MDTA; -.
DR BioCyc; MetaCyc:MDTA; -.
DR PRO; PR:P76397; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:1990281; C:efflux pump complex; IBA:GO_Central.
DR GO; GO:0098567; C:periplasmic side of plasma membrane; IC:ComplexPortal.
DR GO; GO:0015125; F:bile acid transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0015562; F:efflux transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015721; P:bile acid and bile salt transport; IMP:EcoCyc.
DR GO; GO:0140330; P:xenobiotic detoxification by transmembrane export across the cell outer membrane; IC:ComplexPortal.
DR GO; GO:0042908; P:xenobiotic transport; IMP:EcoCyc.
DR HAMAP; MF_01422; MdtA; 1.
DR InterPro; IPR039562; MFP_biotin_lipoyl_2.
DR InterPro; IPR022824; Multidrug-R_MdtA.
DR InterPro; IPR006143; RND_pump_MFP.
DR Pfam; PF13533; Biotin_lipoyl_2; 1.
DR TIGRFAMs; TIGR01730; RND_mfp; 1.
PE 2: Evidence at transcript level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome; Signal;
KW Transport.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..415
FT /note="Multidrug resistance protein MdtA"
FT /id="PRO_0000018699"
FT REGION 32..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 392..415
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 33..49
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 415 AA; 44464 MW; 26240DBBE0DE1A5F CRC64;
MKGSYKSRWV IVIVVVIAAI AAFWFWQGRN DSRSAAPGAT KQAQQSPAGG RRGMRSGPLA
PVQAATAVEQ AVPRYLTGLG TITAANTVTV RSRVDGQLIA LHFQEGQQVK AGDLLAEIDP
SQFKVALAQA QGQLAKDKAT LANARRDLAR YQQLAKTNLV SRQELDAQQA LVSETEGTIK
ADEASVASAQ LQLDWSRITA PVDGRVGLKQ VDVGNQISSG DTTGIVVITQ THPIDLVFTL
PESDIATVVQ AQKAGKPLVV EAWDRTNSKK LSEGTLLSLD NQIDATTGTI KVKARFNNQD
DALFPNQFVN ARMLVDTEQN AVVIPTAALQ MGNEGHFVWV LNSENKVSKH LVTPGIQDSQ
KVVIRAGISA GDRVVTDGID RLTEGAKVEV VEAQSATTPE EKATSREYAK KGARS
