MDTH_ECO57
ID MDTH_ECO57 Reviewed; 402 AA.
AC Q7AFA1; Q8X8N0;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 2.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Multidrug resistance protein MdtH {ECO:0000255|HAMAP-Rule:MF_01529};
GN Name=mdtH {ECO:0000255|HAMAP-Rule:MF_01529};
GN OrderedLocusNames=Z1702, ECs1443;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Confers resistance to norfloxacin and enoxacin.
CC {ECO:0000255|HAMAP-Rule:MF_01529}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01529}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01529}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. DHA1 family.
CC MdtH (TC 2.A.1.2.21) subfamily. {ECO:0000255|HAMAP-Rule:MF_01529}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG55811.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAB34866.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE005174; AAG55811.1; ALT_INIT; Genomic_DNA.
DR EMBL; BA000007; BAB34866.1; ALT_INIT; Genomic_DNA.
DR PIR; C99809; C99809.
DR PIR; G85668; G85668.
DR RefSeq; NP_309470.2; NC_002695.1.
DR RefSeq; WP_000092209.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; Q7AFA1; -.
DR SMR; Q7AFA1; -.
DR STRING; 155864.EDL933_1641; -.
DR EnsemblBacteria; AAG55811; AAG55811; Z1702.
DR EnsemblBacteria; BAB34866; BAB34866; ECs_1443.
DR GeneID; 912474; -.
DR KEGG; ece:Z1702; -.
DR KEGG; ecs:ECs_1443; -.
DR PATRIC; fig|386585.9.peg.1544; -.
DR eggNOG; COG0477; Bacteria.
DR HOGENOM; CLU_001265_60_2_6; -.
DR OMA; TVCVWTL; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR HAMAP; MF_01529; MFS_MdtH; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR022855; Multidrug-R_MdtH.
DR Pfam; PF07690; MFS_1; 2.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..402
FT /note="Multidrug resistance protein MdtH"
FT /id="PRO_0000173343"
FT TOPO_DOM 1..12
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TOPO_DOM 34..98
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TRANSMEM 99..116
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TOPO_DOM 117..138
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TOPO_DOM 160..164
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TOPO_DOM 186..213
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TOPO_DOM 235..243
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TOPO_DOM 265..276
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TRANSMEM 277..297
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TOPO_DOM 298..299
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TOPO_DOM 321..339
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TRANSMEM 340..360
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TOPO_DOM 361..367
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TRANSMEM 368..388
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
FT TOPO_DOM 389..402
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01529"
SQ SEQUENCE 402 AA; 44391 MW; F0AACB64CD012BAA CRC64;
MSRVSQARNL GKYFLLIDNM LVVLGFFVVF PLISIRFVDQ MGWAAVMVGI ALGLRQFIQQ
GLGIFGGAIA DRFGAKPMIV TGMLMRAAGF ATMGIAHEPW LLWFSCLLSG LGGTLFDPPR
SALVVKLIRP QQRGRFFSLL MMQDSAGAVI GALLGSWLLQ YDFRLVCATG AVLFVLCAAF
NAWLLPAWKL STVRTPVREG MTRVMRDKRF VTYVLTLAGY YMLAVQVMLM LPIMVNDVAG
APSAVKWMYA IEACLSLTLL YPIARWSEKH FRLEHRLMAG LLIMSLSMMP VGMVSGLQQL
FTLICLFYIG SIIAEPARET LSASLADARA RGSYMGFSRL GLAIGGAIGY IGGGWLFDLG
KSVHQPELPW MMLGIIGIFT FLALGWQFSQ KRAARRLLER DA
