MDTI_ECOLI
ID MDTI_ECOLI Reviewed; 109 AA.
AC P69210; P77670;
DT 15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Spermidine export protein MdtI;
GN Name=mdtI; Synonyms=ydgE; OrderedLocusNames=b1599, JW1591;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION.
RX PubMed=11566977; DOI=10.1128/jb.183.20.5803-5812.2001;
RA Nishino K., Yamaguchi A.;
RT "Analysis of a complete library of putative drug transporter genes in
RT Escherichia coli.";
RL J. Bacteriol. 183:5803-5812(2001).
RN [5]
RP TOPOLOGY.
RC STRAIN=K12 / JM109 / ATCC 53323;
RX PubMed=11867724; DOI=10.1073/pnas.052018199;
RA Drew D., Sjoestrand D., Nilsson J., Urbig T., Chin C.-N., de Gier J.-W.,
RA von Heijne G.;
RT "Rapid topology mapping of Escherichia coli inner-membrane proteins by
RT prediction and PhoA/GFP fusion analysis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:2690-2695(2002).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [7]
RP FUNCTION, SUBUNIT, INDUCTION, AND MUTAGENESIS OF GLU-5; GLU-19; ASP-60;
RP TRP-68 AND TRP-81.
RC STRAIN=K12 / C600 / CR34 / ATCC 23724 / DSM 3925 / LMG 3041 / NCIB 10222;
RX PubMed=18039771; DOI=10.1128/jb.01505-07;
RA Higashi K., Ishigure H., Demizu R., Uemura T., Nishino K., Yamaguchi A.,
RA Kashiwagi K., Igarashi K.;
RT "Identification of a spermidine excretion protein complex (MdtJI) in
RT Escherichia coli.";
RL J. Bacteriol. 190:872-878(2008).
CC -!- FUNCTION: Catalyzes the excretion of spermidine. Can also confer
CC resistance to deoxycholate and SDS. {ECO:0000269|PubMed:11566977,
CC ECO:0000269|PubMed:18039771}.
CC -!- SUBUNIT: Forms a complex with MdtJ. {ECO:0000305|PubMed:18039771}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- INDUCTION: Induced by spermidine. {ECO:0000269|PubMed:18039771}.
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family. MdtI
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01597, ECO:0000305}.
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DR EMBL; U00096; AAC74671.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15333.1; -; Genomic_DNA.
DR PIR; A64916; A64916.
DR RefSeq; NP_416116.1; NC_000913.3.
DR RefSeq; WP_000046661.1; NZ_STEB01000003.1.
DR AlphaFoldDB; P69210; -.
DR SMR; P69210; -.
DR BioGRID; 4259124; 494.
DR ComplexPortal; CPX-6020; MdtIJ spermidine export complex.
DR IntAct; P69210; 1.
DR STRING; 511145.b1599; -.
DR TCDB; 2.A.7.1.9; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; P69210; -.
DR PRIDE; P69210; -.
DR DNASU; 947333; -.
DR EnsemblBacteria; AAC74671; AAC74671; b1599.
DR EnsemblBacteria; BAA15333; BAA15333; BAA15333.
DR GeneID; 66674510; -.
DR GeneID; 947333; -.
DR KEGG; ecj:JW1591; -.
DR KEGG; eco:b1599; -.
DR PATRIC; fig|1411691.4.peg.663; -.
DR EchoBASE; EB3685; -.
DR eggNOG; COG2076; Bacteria.
DR HOGENOM; CLU_133067_0_4_6; -.
DR InParanoid; P69210; -.
DR OMA; VAAGWIM; -.
DR PhylomeDB; P69210; -.
DR BioCyc; EcoCyc:B1599-MON; -.
DR BioCyc; MetaCyc:B1599-MON; -.
DR PRO; PR:P69210; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoliWiki.
DR GO; GO:1902495; C:transmembrane transporter complex; IPI:ComplexPortal.
DR GO; GO:0015199; F:amino-acid betaine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0015220; F:choline transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015606; F:spermidine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015871; P:choline transport; IBA:GO_Central.
DR GO; GO:0031460; P:glycine betaine transport; IBA:GO_Central.
DR GO; GO:1903711; P:spermidine transmembrane transport; IDA:ComplexPortal.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR GO; GO:1990961; P:xenobiotic detoxification by transmembrane export across the plasma membrane; IMP:EcoCyc.
DR HAMAP; MF_01597; MdtI; 1.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR InterPro; IPR045324; Small_multidrug_res.
DR InterPro; IPR023737; Spermidine_export_MdtI.
DR PANTHER; PTHR30561; PTHR30561; 1.
DR PANTHER; PTHR30561:SF6; PTHR30561:SF6; 1.
DR Pfam; PF00893; Multi_Drug_Res; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..109
FT /note="Spermidine export protein MdtI"
FT /id="PRO_0000108075"
FT TOPO_DOM 1..5
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 27..35
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 57..63
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 85..87
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT MUTAGEN 5
FT /note="E->Q: Decrease in spermidine excretion."
FT /evidence="ECO:0000269|PubMed:18039771"
FT MUTAGEN 19
FT /note="E->Q: Decrease in spermidine excretion."
FT /evidence="ECO:0000269|PubMed:18039771"
FT MUTAGEN 60
FT /note="D->N: Decrease in spermidine excretion."
FT /evidence="ECO:0000269|PubMed:18039771"
FT MUTAGEN 68
FT /note="W->L: Decrease in spermidine excretion."
FT /evidence="ECO:0000269|PubMed:18039771"
FT MUTAGEN 81
FT /note="W->L: Decrease in spermidine excretion."
FT /evidence="ECO:0000269|PubMed:18039771"
SQ SEQUENCE 109 AA; 11720 MW; BBDF61985CB7AE97 CRC64;
MAQFEWVHAA WLALAIVLEI VANVFLKFSD GFRRKIFGLL SLAAVLAAFS ALSQAVKGID
LSVAYALWGG FGIAATLAAG WILFGQRLNR KGWIGLVLLL AGMIMVKLA
