ID   MDTJ_ECO57              Reviewed;         121 AA.
AC   P69213; P77412;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Spermidine export protein MdtJ;
GN   Name=mdtJ; OrderedLocusNames=Z2594, ECs2306;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Catalyzes the excretion of spermidine. {ECO:0000250}.
CC   -!- SUBUNIT: Forms a complex with MdtI. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC       superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family. MdtJ
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01598}.
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DR   EMBL; AE005174; AAG56587.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB35729.1; -; Genomic_DNA.
DR   PIR; B90917; B90917.
DR   PIR; G85765; G85765.
DR   RefSeq; NP_310333.1; NC_002695.1.
DR   RefSeq; WP_000276149.1; NZ_SWKA01000004.1.
DR   AlphaFoldDB; P69213; -.
DR   SMR; P69213; -.
DR   STRING; 155864.EDL933_2551; -.
DR   TCDB; 2.A.7.1.9; the drug/metabolite transporter (dmt) superfamily.
DR   EnsemblBacteria; AAG56587; AAG56587; Z2594.
DR   EnsemblBacteria; BAB35729; BAB35729; ECs_2306.
DR   GeneID; 66674509; -.
DR   GeneID; 913399; -.
DR   KEGG; ece:Z2594; -.
DR   KEGG; ecs:ECs_2306; -.
DR   PATRIC; fig|386585.9.peg.2416; -.
DR   eggNOG; COG2076; Bacteria.
DR   HOGENOM; CLU_133067_0_0_6; -.
DR   OMA; MRSWIYL; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0015606; F:spermidine transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR   HAMAP; MF_01598; MdtJ; 1.
DR   InterPro; IPR000390; Small_drug/metabolite_transptr.
DR   InterPro; IPR045324; Small_multidrug_res.
DR   InterPro; IPR023740; Spermidine_export_MdtJ.
DR   PANTHER; PTHR30561; PTHR30561; 1.
DR   PANTHER; PTHR30561:SF2; PTHR30561:SF2; 1.
DR   Pfam; PF00893; Multi_Drug_Res; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..121
FT                   /note="Spermidine export protein MdtJ"
FT                   /id="PRO_0000108081"
FT   TOPO_DOM        1
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        23..31
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        53..54
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        55..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        76..81
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        82..102
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        103..121
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   121 AA;  13115 MW;  B24330AF5BB181B5 CRC64;
     MYIYWILLGL AIATEITGTL SMKWASVSEG NGGFILMLVM ISLSYIFLSF AVKKIALGVA
     YALWEGIGIL FITLFSVLLF DESLSLMKIA GLTTLVAGIV LIKSGTRKAR KPELEVNHGA
     V