MDTJ_ECOLI
ID MDTJ_ECOLI Reviewed; 121 AA.
AC P69212; P77412;
DT 15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Spermidine export protein MdtJ;
GN Name=mdtJ; Synonyms=ydgF; OrderedLocusNames=b1600, JW1592;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION.
RX PubMed=11566977; DOI=10.1128/jb.183.20.5803-5812.2001;
RA Nishino K., Yamaguchi A.;
RT "Analysis of a complete library of putative drug transporter genes in
RT Escherichia coli.";
RL J. Bacteriol. 183:5803-5812(2001).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [6]
RP FUNCTION, SUBUNIT, INDUCTION, AND MUTAGENESIS OF TYR-4; TRP-5; GLU-15;
RP TYR-45; TYR-61 AND GLU-82.
RC STRAIN=K12 / C600 / CR34 / ATCC 23724 / DSM 3925 / LMG 3041 / NCIB 10222;
RX PubMed=18039771; DOI=10.1128/jb.01505-07;
RA Higashi K., Ishigure H., Demizu R., Uemura T., Nishino K., Yamaguchi A.,
RA Kashiwagi K., Igarashi K.;
RT "Identification of a spermidine excretion protein complex (MdtJI) in
RT Escherichia coli.";
RL J. Bacteriol. 190:872-878(2008).
CC -!- FUNCTION: Catalyzes the excretion of spermidine. Can also confer
CC resistance to deoxycholate and SDS. {ECO:0000269|PubMed:11566977,
CC ECO:0000269|PubMed:18039771}.
CC -!- SUBUNIT: Forms a complex with MdtI. {ECO:0000305|PubMed:18039771}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- INDUCTION: Induced by spermidine. {ECO:0000269|PubMed:18039771}.
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family. MdtJ
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01598, ECO:0000305}.
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DR EMBL; U00096; AAC74672.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15334.1; -; Genomic_DNA.
DR PIR; B64916; B64916.
DR RefSeq; NP_416117.1; NC_000913.3.
DR RefSeq; WP_000276149.1; NZ_SSZK01000001.1.
DR AlphaFoldDB; P69212; -.
DR SMR; P69212; -.
DR BioGRID; 4260838; 195.
DR ComplexPortal; CPX-6020; MdtIJ spermidine export complex.
DR STRING; 511145.b1600; -.
DR PaxDb; P69212; -.
DR DNASU; 946139; -.
DR EnsemblBacteria; AAC74672; AAC74672; b1600.
DR EnsemblBacteria; BAA15334; BAA15334; BAA15334.
DR GeneID; 66674509; -.
DR GeneID; 946139; -.
DR KEGG; ecj:JW1592; -.
DR KEGG; eco:b1600; -.
DR PATRIC; fig|1411691.4.peg.662; -.
DR EchoBASE; EB3686; -.
DR eggNOG; COG2076; Bacteria.
DR HOGENOM; CLU_133067_0_0_6; -.
DR InParanoid; P69212; -.
DR OMA; MRSWIYL; -.
DR PhylomeDB; P69212; -.
DR BioCyc; EcoCyc:B1600-MON; -.
DR BioCyc; MetaCyc:B1600-MON; -.
DR PRO; PR:P69212; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:1902495; C:transmembrane transporter complex; IPI:ComplexPortal.
DR GO; GO:0015199; F:amino-acid betaine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0015220; F:choline transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015606; F:spermidine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015871; P:choline transport; IBA:GO_Central.
DR GO; GO:0031460; P:glycine betaine transport; IBA:GO_Central.
DR GO; GO:1903711; P:spermidine transmembrane transport; IDA:ComplexPortal.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR GO; GO:1990961; P:xenobiotic detoxification by transmembrane export across the plasma membrane; IMP:EcoCyc.
DR HAMAP; MF_01598; MdtJ; 1.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR InterPro; IPR045324; Small_multidrug_res.
DR InterPro; IPR023740; Spermidine_export_MdtJ.
DR PANTHER; PTHR30561; PTHR30561; 1.
DR PANTHER; PTHR30561:SF2; PTHR30561:SF2; 1.
DR Pfam; PF00893; Multi_Drug_Res; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..121
FT /note="Spermidine export protein MdtJ"
FT /id="PRO_0000108079"
FT TOPO_DOM 1
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 23..31
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..54
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 76..81
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 103..121
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MUTAGEN 4
FT /note="Y->L: Decrease in spermidine excretion."
FT /evidence="ECO:0000269|PubMed:18039771"
FT MUTAGEN 5
FT /note="W->L: Decrease in spermidine excretion."
FT /evidence="ECO:0000269|PubMed:18039771"
FT MUTAGEN 15
FT /note="E->Q: Decrease in spermidine excretion."
FT /evidence="ECO:0000269|PubMed:18039771"
FT MUTAGEN 45
FT /note="Y->L: Decrease in spermidine excretion."
FT /evidence="ECO:0000269|PubMed:18039771"
FT MUTAGEN 61
FT /note="Y->L: Decrease in spermidine excretion."
FT /evidence="ECO:0000269|PubMed:18039771"
FT MUTAGEN 82
FT /note="E->Q: Decrease in spermidine excretion."
FT /evidence="ECO:0000269|PubMed:18039771"
SQ SEQUENCE 121 AA; 13115 MW; B24330AF5BB181B5 CRC64;
MYIYWILLGL AIATEITGTL SMKWASVSEG NGGFILMLVM ISLSYIFLSF AVKKIALGVA
YALWEGIGIL FITLFSVLLF DESLSLMKIA GLTTLVAGIV LIKSGTRKAR KPELEVNHGA
V
