ID   MDTK_ERWAM              Reviewed;         457 AA.
AC   Q7WTR3;
DT   16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Multidrug resistance protein MdtK;
DE   AltName: Full=Multidrug-efflux transporter;
GN   Name=mdtK; Synonyms=norM;
OS   Erwinia amylovora (Fire blight bacteria).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Erwinia.
OX   NCBI_TaxID=552;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=Ea1189 / GSPB 1189;
RX   PubMed=14766544; DOI=10.1128/aem.70.2.693-703.2004;
RA   Burse A., Weingart H., Ullrich M.S.;
RT   "NorM, an Erwinia amylovora multidrug efflux pump involved in in vitro
RT   competition with other epiphytic bacteria.";
RL   Appl. Environ. Microbiol. 70:693-703(2004).
CC   -!- FUNCTION: Multidrug efflux pump that functions probably as a Na(+)/drug
CC       antiporter. Confers resistance to the hydrophobic cationic compounds
CC       norfloxacin, ethidium bromide and berberine. It provides protection
CC       against antibiotics produced by epiphytic bacteria, such as
CC       P.agglomerans, which cocolonize plant blossoms.
CC       {ECO:0000269|PubMed:14766544}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the multi antimicrobial extrusion (MATE) (TC
CC       2.A.66.1) family. MdtK subfamily. {ECO:0000305}.
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DR   EMBL; AY307101; AAQ21212.1; -; Genomic_DNA.
DR   RefSeq; WP_004157415.1; NZ_RQKG01000001.1.
DR   AlphaFoldDB; Q7WTR3; -.
DR   SMR; Q7WTR3; -.
DR   GeneID; 8912318; -.
DR   OMA; EFWILLK; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-UniRule.
DR   GO; GO:0006855; P:xenobiotic transmembrane transport; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00400; MdtK; 1.
DR   InterPro; IPR002528; MATE_fam.
DR   InterPro; IPR022913; Multidrug-R_MdtK.
DR   Pfam; PF01554; MatE; 2.
DR   PIRSF; PIRSF006603; DinF; 1.
DR   TIGRFAMs; TIGR00797; matE; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Antiport; Cell inner membrane; Cell membrane;
KW   Ion transport; Membrane; Sodium; Sodium transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..457
FT                   /note="Multidrug resistance protein MdtK"
FT                   /id="PRO_0000164184"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        93..113
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        127..147
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        159..179
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        188..208
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        243..263
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        276..296
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        316..336
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        357..377
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        386..406
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        418..438
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   457 AA;  49212 MW;  70B32A2FDDFB1302 CRC64;
     MQKYLSEARQ LLALAIPVIL AQVAQTSMSF VDTVMAGAVS ATDMAAVAVG TSIWLPAILF
     GHGLLLALTP VVAQLNGSGR RDLIAHQVRQ AYWLAGMVSV LVMIVLYNAG YLIGSLHNVD
     PLLSQKAIGY LHALLWGAPG YLFFQVARDR CEGLSKTKPA MVVGFLGLLV NIPLNYVFIH
     GHLGMPALGG VGCGVATASV YWVMFIVIRA WTNRAKSMRD IQLPQRFSAP DWKAIGRLTG
     LGLPVALALF FEVTLFAVVA LLVSPLGIVN VAGHQIALNF SSLMFVVPLS LGVATTIRVG
     HRLGQGSAEQ AKVAAWTAQG IGICMACITA LFTIAFRERI AQLYADDPEV ITMAAQLMLL
     AAVYQLSDSV QVIGSGVLRG YKDTRAIFFI TFIAYWILGL PIGYLLGMTN LLLPAMGPAG
     FWIGFIIGLT SSAIMMIWRM RRLQRQPNAT VLARAAR