MDTN_ECO57
ID MDTN_ECO57 Reviewed; 343 AA.
AC Q8X5R2; Q7A913;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Multidrug resistance protein MdtN;
GN Name=mdtN; OrderedLocusNames=Z5682, ECs5064;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Could be involved in resistance to puromycin, acriflavine and
CC tetraphenylarsonium chloride. {ECO:0000250}.
CC -!- SUBUNIT: Could be part of a tripartite efflux system composed of MdtN,
CC MdtO and MdtP. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC type II membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC family. {ECO:0000305}.
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DR EMBL; AE005174; AAG59280.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB38487.1; -; Genomic_DNA.
DR PIR; D86102; D86102.
DR PIR; H91261; H91261.
DR RefSeq; NP_313091.1; NC_002695.1.
DR RefSeq; WP_000446391.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; Q8X5R2; -.
DR SMR; Q8X5R2; -.
DR STRING; 155864.EDL933_5424; -.
DR EnsemblBacteria; AAG59280; AAG59280; Z5682.
DR EnsemblBacteria; BAB38487; BAB38487; ECs_5064.
DR GeneID; 914275; -.
DR KEGG; ece:Z5682; -.
DR KEGG; ecs:ECs_5064; -.
DR PATRIC; fig|386585.9.peg.5292; -.
DR eggNOG; COG1566; Bacteria.
DR HOGENOM; CLU_018816_15_2_6; -.
DR OMA; QRIPMRV; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:1990961; P:xenobiotic detoxification by transmembrane export across the plasma membrane; IEA:InterPro.
DR InterPro; IPR043602; CusB_dom_1.
DR InterPro; IPR005694; Efflux_pump_mem.
DR InterPro; IPR032317; HlyD_D23.
DR InterPro; IPR030196; MdtN.
DR PANTHER; PTHR30367:SF1; PTHR30367:SF1; 1.
DR Pfam; PF00529; CusB_dom_1; 1.
DR Pfam; PF16576; HlyD_D23; 1.
DR TIGRFAMs; TIGR00998; 8a0101; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..343
FT /note="Multidrug resistance protein MdtN"
FT /id="PRO_0000201884"
FT TOPO_DOM 1..12
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 13..33
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 34..343
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 343 AA; 36952 MW; 01956D33CD1773E3 CRC64;
MESTPKKAPR SKFPALLVVA LALVALVFVI WRVDSAPSTN DAYVSADTID VVPEVSGRIV
ELAVTDNQAV KQGDLLFRID PRPYEANLAK SEASLAALDK QIMLTQRSVD AQQFGADSVN
ATVEKARAAA KQATDTLRRT EPLLKEGFVS AEDVDRARTA QRAAEADLNA VLLQAQSAAS
AVSGVDALVA QRAAVEADIA LTKLHLEMAT VRAPFDGRVI SLKTSVGQFA SAMRPIFTLI
DTRHWYVIAN FRETDLKNIR SGTPATIRLM SDSGKTFEGK VDSIGYGVLP DDGGLVLGGL
PKVSRSINWV RVAQRFPVKI MVDKPDPEMF RIGASAVANL EPQ