ID   MDTN_ECOL6              Reviewed;         343 AA.
AC   Q8FAX1;
DT   16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Multidrug resistance protein MdtN;
GN   Name=mdtN; OrderedLocusNames=c5087;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Could be involved in resistance to puromycin, acriflavine and
CC       tetraphenylarsonium chloride. {ECO:0000250}.
CC   -!- SUBUNIT: Could be part of a tripartite efflux system composed of MdtN,
CC       MdtO and MdtP. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       type II membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   EMBL; AE014075; AAN83512.1; -; Genomic_DNA.
DR   RefSeq; WP_000446365.1; NC_004431.1.
DR   AlphaFoldDB; Q8FAX1; -.
DR   SMR; Q8FAX1; -.
DR   STRING; 199310.c5087; -.
DR   EnsemblBacteria; AAN83512; AAN83512; c5087.
DR   KEGG; ecc:c5087; -.
DR   eggNOG; COG1566; Bacteria.
DR   HOGENOM; CLU_018816_15_2_6; -.
DR   OMA; QRIPMRV; -.
DR   BioCyc; ECOL199310:C5087-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:1990961; P:xenobiotic detoxification by transmembrane export across the plasma membrane; IEA:InterPro.
DR   InterPro; IPR043602; CusB_dom_1.
DR   InterPro; IPR005694; Efflux_pump_mem.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR030196; MdtN.
DR   PANTHER; PTHR30367:SF1; PTHR30367:SF1; 1.
DR   Pfam; PF00529; CusB_dom_1; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   TIGRFAMs; TIGR00998; 8a0101; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW   Signal-anchor; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..343
FT                   /note="Multidrug resistance protein MdtN"
FT                   /id="PRO_0000201885"
FT   TOPO_DOM        1..12
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        13..33
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        34..343
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   343 AA;  36936 MW;  9587B5C4260CD872 CRC64;
     MESTPKKAPR SKFPALLVVA LALVALVFVI WRVDSAPSTN DAYASADTID VVPEVSGRIV
     ELAVTDNQAV KQGDLLFRID PRPYEANLAK AEASLAALDK QIMLTQRSVD AQQFGADSVN
     ATVEKARAAA KQASDTLRRT EPLLREGFVS AEEVDRARTA QRAAEADLNA VLLQAQSAAS
     AVSGVDALVA QRAAVEADIA LTKLHLEMAT VRAPFDGRVI SLKTSVGQFA SAMRPIFTLI
     DTRHWYVIAN FRETDLKNIR SGTPATIRLM SDSGKTFEGK VDSIGYGVLP DDGGLVLGGL
     PKVSRSINWV RVAQRFPVKI MVDKPDPEMF RIGASAVANL EPQ