MDTN_ECOLI
ID MDTN_ECOLI Reviewed; 343 AA.
AC P32716; Q2M6M2;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Multidrug resistance protein MdtN;
GN Name=mdtN; Synonyms=yjcR; OrderedLocusNames=b4082, JW4043;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8265357; DOI=10.1093/nar/21.23.5408;
RA Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.;
RT "Analysis of the Escherichia coli genome. IV. DNA sequence of the region
RT from 89.2 to 92.8 minutes.";
RL Nucleic Acids Res. 21:5408-5417(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP PUTATIVE FUNCTION.
RX PubMed=11257026; DOI=10.1128/aac.45.4.1126-1136.2001;
RA Sulavik M.C., Houseweart C., Cramer C., Jiwani N., Murgolo N., Greene J.,
RA DiDomenico B., Shaw K.J., Miller G.H., Hare R., Shimer G.;
RT "Antibiotic susceptibility profiles of Escherichia coli strains lacking
RT multidrug efflux pump genes.";
RL Antimicrob. Agents Chemother. 45:1126-1136(2001).
RN [5]
RP OPERON STRUCTURE, AND INDUCTION.
RC STRAIN=K12 / BW25113;
RX PubMed=19429622; DOI=10.1128/jb.00108-09;
RA Shimada T., Yamamoto K., Ishihama A.;
RT "Involvement of the leucine response transcription factor LeuO in
RT regulation of the genes for sulfa drug efflux.";
RL J. Bacteriol. 191:4562-4571(2009).
CC -!- FUNCTION: Could be involved in resistance to puromycin, acriflavine and
CC tetraphenylarsonium chloride.
CC -!- SUBUNIT: Could be part of a tripartite efflux system composed of MdtN,
CC MdtO and MdtP.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC type II membrane protein {ECO:0000305}.
CC -!- INDUCTION: Induced by LeuO, part of the mdtNO operon.
CC {ECO:0000269|PubMed:19429622}.
CC -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC family. {ECO:0000305}.
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DR EMBL; U00006; AAC43176.1; -; Genomic_DNA.
DR EMBL; U00096; AAD13465.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78084.1; -; Genomic_DNA.
DR PIR; A65217; A65217.
DR RefSeq; NP_418506.1; NC_000913.3.
DR RefSeq; WP_000446378.1; NZ_SSZK01000016.1.
DR AlphaFoldDB; P32716; -.
DR SMR; P32716; -.
DR BioGRID; 4262953; 208.
DR ComplexPortal; CPX-6021; SdsRQP multidrug transport complex.
DR IntAct; P32716; 3.
DR STRING; 511145.b4082; -.
DR PaxDb; P32716; -.
DR PRIDE; P32716; -.
DR EnsemblBacteria; AAD13465; AAD13465; b4082.
DR EnsemblBacteria; BAE78084; BAE78084; BAE78084.
DR GeneID; 948598; -.
DR KEGG; ecj:JW4043; -.
DR KEGG; eco:b4082; -.
DR PATRIC; fig|1411691.4.peg.2619; -.
DR EchoBASE; EB1897; -.
DR eggNOG; COG1566; Bacteria.
DR HOGENOM; CLU_018816_15_2_6; -.
DR InParanoid; P32716; -.
DR OMA; QRIPMRV; -.
DR PhylomeDB; P32716; -.
DR BioCyc; EcoCyc:EG11954-MON; -.
DR BioCyc; MetaCyc:EG11954-MON; -.
DR PRO; PR:P32716; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:1990351; C:transporter complex; IC:ComplexPortal.
DR GO; GO:0015546; F:sulfathiazole transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IMP:EcoCyc.
DR GO; GO:1902599; P:sulfathiazole transmembrane transport; IMP:EcoCyc.
DR GO; GO:0055085; P:transmembrane transport; IC:ComplexPortal.
DR GO; GO:1990961; P:xenobiotic detoxification by transmembrane export across the plasma membrane; IEA:InterPro.
DR InterPro; IPR043602; CusB_dom_1.
DR InterPro; IPR005694; Efflux_pump_mem.
DR InterPro; IPR032317; HlyD_D23.
DR InterPro; IPR030196; MdtN.
DR PANTHER; PTHR30367:SF1; PTHR30367:SF1; 1.
DR Pfam; PF00529; CusB_dom_1; 1.
DR Pfam; PF16576; HlyD_D23; 1.
DR TIGRFAMs; TIGR00998; 8a0101; 1.
PE 2: Evidence at transcript level;
KW Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..343
FT /note="Multidrug resistance protein MdtN"
FT /id="PRO_0000201883"
FT TOPO_DOM 1..12
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 13..33
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 34..343
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 343 AA; 36908 MW; C599B8707C475FFE CRC64;
MESTPKKAPR SKFPALLVVA LALVALVFVI WRVDSAPSTN DAYASADTID VVPEVSGRIV
ELAVTDNQAV KQGDLLFRID PRPYEANLAK AEASLAALDK QIMLTQRSVD AQQFGADSVN
ATVEKARAAA KQATDTLRRT EPLLKEGFVS AEDVDRARTA QRAAEADLNA VLLQAQSAAS
AVSGVDALVA QRAAVEADIA LTKLHLEMAT VRAPFDGRVI SLKTSVGQFA SAMRPIFTLI
DTRHWYVIAN FRETDLKNIR SGTPATIRLM SDSGKTFEGK VDSIGYGVLP DDGGLVLGGL
PKVSRSINWV RVAQRFPVKI MVDKPDPEMF RIGASAVANL EPQ
