ID   MDTN_SHIFL              Reviewed;         343 AA.
AC   Q83P86; Q7UBB7;
DT   16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 4.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Multidrug resistance protein MdtN;
GN   Name=mdtN; OrderedLocusNames=SF4142, S3608;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Could be involved in resistance to puromycin, acriflavine and
CC       tetraphenylarsonium chloride. {ECO:0000250}.
CC   -!- SUBUNIT: Could be part of a tripartite efflux system composed of MdtN,
CC       MdtO and MdtP. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       type II membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   EMBL; AE005674; AAN45564.2; -; Genomic_DNA.
DR   EMBL; AE014073; AAP18652.1; -; Genomic_DNA.
DR   RefSeq; NP_709857.2; NC_004337.2.
DR   RefSeq; WP_000446384.1; NZ_WPGW01000075.1.
DR   AlphaFoldDB; Q83P86; -.
DR   SMR; Q83P86; -.
DR   STRING; 198214.SF4142; -.
DR   EnsemblBacteria; AAN45564; AAN45564; SF4142.
DR   EnsemblBacteria; AAP18652; AAP18652; S3608.
DR   GeneID; 1025617; -.
DR   GeneID; 58390004; -.
DR   KEGG; sfl:SF4142; -.
DR   KEGG; sfx:S3608; -.
DR   PATRIC; fig|198214.7.peg.4889; -.
DR   HOGENOM; CLU_018816_15_2_6; -.
DR   OMA; QRIPMRV; -.
DR   OrthoDB; 757027at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:1990961; P:xenobiotic detoxification by transmembrane export across the plasma membrane; IEA:InterPro.
DR   InterPro; IPR043602; CusB_dom_1.
DR   InterPro; IPR005694; Efflux_pump_mem.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR030196; MdtN.
DR   PANTHER; PTHR30367:SF1; PTHR30367:SF1; 1.
DR   Pfam; PF00529; CusB_dom_1; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   TIGRFAMs; TIGR00998; 8a0101; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..343
FT                   /note="Multidrug resistance protein MdtN"
FT                   /id="PRO_0000201886"
FT   TOPO_DOM        1..12
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        13..33
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        34..343
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   343 AA;  36952 MW;  D598AAE061D64C8F CRC64;
     MESTPKKAPR SKFPALLVVA LALVALVFVI WRVDSAPSTN DAYASADTID VVPEVSGRIV
     ELAVTDNQAV KQGDLLFRID PRPYEANLAK AEASLAALDK QIMLTQRSVD AQQFGADSVN
     ATVEKARAAA KQATDTLRRT EPLLKEGFVS AEDVDRARTA QRAAEADLNA VLLQAQSAAS
     AVSGVDALVA QRAAVEADIA LTKLHLEMTT VRAPFDGRII SLKTSVGQFA SAMRPIFTLI
     DTRHWYVIAN FRETDLKNIR SGTPATIRLM SDSGKTFEGK VDSIGYGVLP DDGGLVLGGL
     PKVSRSINWV RVAQRFPVKI MVDKPDPEMF RIGASAVANL EPQ