ID   MDTO_ECO57              Reviewed;         683 AA.
AC   Q8X5R8; Q7A914;
DT   16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2005, sequence version 2.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Multidrug resistance protein MdtO;
GN   Name=mdtO; OrderedLocusNames=Z5681, ECs5063;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Could be involved in resistance to puromycin, acriflavine and
CC       tetraphenylarsonium chloride. {ECO:0000250}.
CC   -!- SUBUNIT: Could be part of a tripartite efflux system composed of MdtN,
CC       MdtO and MdtP. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MdtO family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG59279.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=BAB38486.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE005174; AAG59279.1; ALT_INIT; Genomic_DNA.
DR   EMBL; BA000007; BAB38486.1; ALT_INIT; Genomic_DNA.
DR   PIR; C86102; C86102.
DR   PIR; G91261; G91261.
DR   RefSeq; NP_313090.2; NC_002695.1.
DR   RefSeq; WP_001275213.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; Q8X5R8; -.
DR   STRING; 155864.EDL933_5423; -.
DR   EnsemblBacteria; AAG59279; AAG59279; Z5681.
DR   EnsemblBacteria; BAB38486; BAB38486; ECs_5063.
DR   GeneID; 914278; -.
DR   KEGG; ece:Z5681; -.
DR   KEGG; ecs:ECs_5063; -.
DR   PATRIC; fig|386585.9.peg.5291; -.
DR   eggNOG; COG1289; Bacteria.
DR   HOGENOM; CLU_023392_1_0_6; -.
DR   OMA; QSDWRIT; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR006726; PHBA_efflux_AaeB/fusaric-R.
DR   Pfam; PF04632; FUSC; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..683
FT                   /note="Multidrug resistance protein MdtO"
FT                   /id="PRO_0000210091"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        75..95
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        125..145
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        158..178
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        402..422
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        426..446
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        457..477
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        483..503
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   683 AA;  76119 MW;  785222852F6CCB88 CRC64;
     MSALNSLPLP VVRLLAFFHE ELSERRPGRV PQTVQLWVGC LLVILISMTF EIPFVALSLA
     VLFYGIQSNA FYTKFVAILF VVATVLEIGS LFLIYKWSYG EPLIRLIIAG PILMGCMFLM
     RTHRLGLVFF AVAIVAIYGQ TFPAMLDYPE VVVRLTLWCI VVGLYPTLLM TLIGVLWFPS
     RAISQMHQAL NDRLDDAISH LTDSLAPLPE TRIEREALAL QKLNVFCLAD DANWRTQSAW
     WQSCVATVTY IYSTLNRYDP TSFADSQAII EFRQKLASEI NKLQHAITEG QCWQSDWRIS
     ESEAMAAREC NLENICQTLL QLGQMDPNTP PTPAAKPPSM VADAFTNPDY MRYAVKTLLA
     CLICYTFYSG VDWEGIHTCM LTCVIVANPN VGSSYQKMVL RFGGAFCGAI LALLFTLLVM
     PWLDNIVELL FVLAPIFLLG AWIATSSERS SYIGTQMVVT FALATLENVF GPVYDLVEIR
     DRALGIIIGT VVSAVIYTFV WPESEARTLP QKLAGALGML SKVMRIPRQQ EVTALRTYLQ
     IRIGLHAAFN ACEEMCQRVA LERQLDSEER ALLIERSQTV IHQGRDLLHA WDATWNSAQA
     LDNALQPDKA GQFADALEKY AAGLATALSR SPQITLEETP ASQAILPTLL KQEQHVCQLF
     ARLPDWTAPA LTPATEQAQG ATQ