ID   MDTO_SHIFL              Reviewed;         680 AA.
AC   Q83IQ8; Q7UBB8;
DT   16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2005, sequence version 5.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Multidrug resistance protein MdtO;
GN   Name=mdtO; OrderedLocusNames=SF4141, S2587;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Could be involved in resistance to puromycin, acriflavine and
CC       tetraphenylarsonium chloride. {ECO:0000250}.
CC   -!- SUBUNIT: Could be part of a tripartite efflux system composed of MdtN,
CC       MdtO and MdtP. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MdtO family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN45563.2; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAP18651.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE005674; AAN45563.2; ALT_INIT; Genomic_DNA.
DR   EMBL; AE014073; AAP18651.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_709856.4; NC_004337.2.
DR   RefSeq; WP_001275151.1; NZ_WPGU01000069.1.
DR   AlphaFoldDB; Q83IQ8; -.
DR   STRING; 198214.SF4141; -.
DR   EnsemblBacteria; AAN45563; AAN45563; SF4141.
DR   EnsemblBacteria; AAP18651; AAP18651; S2587.
DR   GeneID; 1025729; -.
DR   KEGG; sfl:SF4141; -.
DR   KEGG; sft:NCTC1_04505; -.
DR   KEGG; sfx:S2587; -.
DR   PATRIC; fig|198214.7.peg.4888; -.
DR   HOGENOM; CLU_023392_1_0_6; -.
DR   OrthoDB; 392748at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR006726; PHBA_efflux_AaeB/fusaric-R.
DR   Pfam; PF04632; FUSC; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..680
FT                   /note="Multidrug resistance protein MdtO"
FT                   /id="PRO_0000210092"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        75..95
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        125..145
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        155..175
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        399..419
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        423..443
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        454..474
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        480..500
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   680 AA;  75903 MW;  C122778AC02C317F CRC64;
     MSALNSLPLP VVRLLAFFHE ELSERRPGRV PQIVQLWVGC LLVILISMTF EIPFVALSLA
     VLFYGIQSNA FYTKFVAILF VVATVLEIGS LFLIYKWSYG EPLIRLIIAG PILMGCMFLM
     RTHRLGLVFF AVAIYGQTFP AMLDYPEVVV RLTLWCIVVG LYPTLLMTLI GVLWFPNRAI
     TQMHQALNDR LDDAISHLTD SLAPLPETRI EREALALQKL NVFCLADDAN WRTQSAWWQS
     CVATVTYIYS TLNRYDPTSF ADSQAIIEFR QKLASEINKL QHAVAEGQCW QSDWRLSESE
     AVAARECNLE NICQTLLQLG QMNPNTPPTP AAKPPSMVAD AFTNPDYIRY AVKTLLACLI
     CYTFYSGVDW EGIHTCMLTC VIVANPNVGS SYQKMVLRFG GAFCGAILAL LFTLLVMPWL
     DNIVELLFVL APIFLLGAWI ATSSERSSYI GTQMVVTFAL ATLENVFGPV YDLVEIRDRA
     LGIIIGTVVS AMIYTFVWPE SEARTLPQKL AGALGMLSKV MRIPRQQEVT ALRTYLQIRI
     GLHAAFNACE EMCQRVALER QLDSEERALL IERSQTVIRQ GRDILHAWDA TWNSAQALDN
     ALQPDRAGQF ADALEKYAAG LATALSRSPQ ITLEETPTSQ AILPTLLKQE QHVCQLFARL
     PDWTAPALTP ATEQAQGATQ