ID   ME53_NPVAC              Reviewed;         449 AA.
AC   Q04719;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   29-SEP-2021, entry version 57.
DE   RecName: Full=Early 53 kDa protein;
DE   AltName: Full=ME-53;
GN   Name=ME53;
OS   Autographa californica nuclear polyhedrosis virus (AcMNPV).
OC   Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX   NCBI_TaxID=46015;
OH   NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=E;
RX   PubMed=8093490; DOI=10.1128/jvi.67.2.753-758.1993;
RA   Knebel-Moersdorf D., Kremer A., Jahnel F.;
RT   "Baculovirus gene ME53, which contains a putative zinc finger motif, is one
RT   of the major early-transcribed genes.";
RL   J. Virol. 67:753-758(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C6;
RX   PubMed=8030224; DOI=10.1006/viro.1994.1380;
RA   Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D.;
RT   "The complete DNA sequence of Autographa californica nuclear polyhedrosis
RT   virus.";
RL   Virology 202:586-605(1994).
RN   [3]
RP   SUBCELLULAR LOCATION.
RX   PubMed=19457997; DOI=10.1128/jvi.02390-08;
RA   de Jong J., Arif B.M., Theilmann D.A., Krell P.J.;
RT   "Autographa californica multiple nucleopolyhedrovirus me53 (ac140) is a
RT   nonessential gene required for efficient budded-virus production.";
RL   J. Virol. 83:7440-7448(2009).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=21775466; DOI=10.1128/jvi.00833-11;
RA   de Jong J., Theilmann D.A., Arif B.M., Krell P.J.;
RT   "Immediate-early protein ME53 forms foci and colocalizes with GP64 and the
RT   major capsid protein VP39 at the cell membranes of Autographa californica
RT   multiple nucleopolyhedrovirus-infected cells.";
RL   J. Virol. 85:9696-9707(2011).
RN   [5]
RP   FUNCTION.
RX   PubMed=26842471; DOI=10.1128/jvi.03115-15;
RA   Liu Y., de Jong J., Nagy E., Theilmann D.A., Krell P.J.;
RT   "Nuclear Translocation Sequence and Region in Autographa californica
RT   Multiple Nucleopolyhedrovirus ME53 That Are Important for Optimal
RT   Baculovirus Production.";
RL   J. Virol. 90:3953-3965(2016).
CC   -!- FUNCTION: May act as a packaging protein or as a structural component
CC       associated with intranuclear baculovirus virion assembly.
CC       {ECO:0000269|PubMed:26842471}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:19457997}. Host
CC       cytoplasm {ECO:0000269|PubMed:19457997, ECO:0000269|PubMed:21775466}.
CC       Host nucleus {ECO:0000269|PubMed:19457997,
CC       ECO:0000269|PubMed:21775466}. Host cell membrane
CC       {ECO:0000269|PubMed:21775466}. Note=Colocalizes with protein GP64.
CC       {ECO:0000269|PubMed:21775466}.
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DR   EMBL; L05914; AAA46718.1; -; Genomic_DNA.
DR   EMBL; L22858; AAA66769.1; -; Genomic_DNA.
DR   PIR; A45709; A45709.
DR   RefSeq; NP_054169.1; NC_001623.1.
DR   PRIDE; Q04719; -.
DR   GeneID; 1403972; -.
DR   KEGG; vg:1403972; -.
DR   Proteomes; UP000008292; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   InterPro; IPR010336; Baculo_ME53.
DR   Pfam; PF06061; Baculo_ME53; 1.
PE   4: Predicted;
KW   DNA-binding; Early protein; Host cell membrane; Host cytoplasm;
KW   Host membrane; Host nucleus; Membrane; Metal-binding; Reference proteome;
KW   Virion; Zinc; Zinc-finger.
FT   CHAIN           1..449
FT                   /note="Early 53 kDa protein"
FT                   /id="PRO_0000132844"
FT   ZN_FING         379..399
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255"
FT   REGION          1..68
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        29..60
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        58..59
FT                   /note="MC -> TR (in Ref. 1; AAA46718)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   449 AA;  52636 MW;  322CF0EFCD0B0865 CRC64;
     MNRFFRENNI FDAPRTGGKG RAKSLPAPAA NSPPSPVRPP PKSNIKPPTR ISPPKQPMCT
     SPAKPLEHSS IVSKKPVVNR KDGYFVPPEF GNKFEGLPAY SDKLDFKQER DLRMHFMSDL
     ERDIMKATLK FSTNYIMGYI NSKDMRMTGK FASRPVKYKK TMEHMSDSRC TTCNYRFKDN
     TREWFLYVVV HIEKPLDDPD RIDICCQKCY LYHNVPKTSY EIYPSINLVD LSYLARERFF
     YQYIFPVSLE HTTEVKELRI DDHNCKVFEI IRRIIRNHKE PNERIQTIDL STTGGLVLRE
     TYTNIVLQRY RSMCTRPDVV DDVNCFILQE PSEMMAALQD NRFSGIKGTV FATVKVKKFT
     QVLDGAITFP LKPTTNNYCK LCKKTKLYYK NPVLYCTKCG FTNVYHFPEY SKFMYYFEAI
     KSFEMHNEMI IYYDLKMYKK LINIVNNNV