MEAK7_MOUSE
ID MEAK7_MOUSE Reviewed; 455 AA.
AC Q8K0P3; Q69ZF1;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=MTOR-associated protein MEAK7 {ECO:0000305};
DE Short=MEAK7 {ECO:0000305};
DE AltName: Full=TBC/LysM-associated domain-containing protein 1;
DE AltName: Full=TLD domain-containing protein 1;
GN Name=Meak7; Synonyms=Kiaa1609, Tldc1 {ECO:0000312|MGI:MGI:1921597};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Pancreatic islet;
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Urinary bladder;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Activates an alternative mTOR signaling through RPS6KB2
CC activation and EIF4EBP1 repression to regulate cell proliferation and
CC migration. Recruits MTOR at the lysosome, essential for MTOR signaling
CC at the lysosome. {ECO:0000250|UniProtKB:Q6P9B6}.
CC -!- SUBUNIT: Interacts (via C-terminal domain) with MTOR and MLST8; the
CC interaction with MTOR increases upon nutrient stimulation.
CC {ECO:0000250|UniProtKB:Q6P9B6}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q6P9B6}.
CC Cytoplasm {ECO:0000250|UniProtKB:Q6P9B6}. Lysosome
CC {ECO:0000250|UniProtKB:Q6P9B6}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8K0P3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8K0P3-2; Sequence=VSP_030079, VSP_030080;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD32493.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAD32493.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK173215; BAD32493.1; ALT_SEQ; mRNA.
DR EMBL; AK079186; BAC37572.1; -; mRNA.
DR EMBL; BC030874; AAH30874.1; -; mRNA.
DR CCDS; CCDS40494.1; -. [Q8K0P3-1]
DR RefSeq; NP_083159.1; NM_028883.2. [Q8K0P3-1]
DR AlphaFoldDB; Q8K0P3; -.
DR SMR; Q8K0P3; -.
DR STRING; 10090.ENSMUSP00000044430; -.
DR iPTMnet; Q8K0P3; -.
DR PhosphoSitePlus; Q8K0P3; -.
DR EPD; Q8K0P3; -.
DR jPOST; Q8K0P3; -.
DR MaxQB; Q8K0P3; -.
DR PaxDb; Q8K0P3; -.
DR PeptideAtlas; Q8K0P3; -.
DR PRIDE; Q8K0P3; -.
DR ProteomicsDB; 259457; -. [Q8K0P3-1]
DR Antibodypedia; 48850; 224 antibodies from 18 providers.
DR Ensembl; ENSMUST00000049156; ENSMUSP00000044430; ENSMUSG00000034105. [Q8K0P3-1]
DR GeneID; 74347; -.
DR KEGG; mmu:74347; -.
DR UCSC; uc009nqh.1; mouse. [Q8K0P3-1]
DR CTD; 57707; -.
DR MGI; MGI:1921597; Meak7.
DR VEuPathDB; HostDB:ENSMUSG00000034105; -.
DR eggNOG; KOG2557; Eukaryota.
DR GeneTree; ENSGT00940000158087; -.
DR HOGENOM; CLU_036763_2_0_1; -.
DR InParanoid; Q8K0P3; -.
DR OMA; VIEDWVF; -.
DR OrthoDB; 585082at2759; -.
DR PhylomeDB; Q8K0P3; -.
DR TreeFam; TF316541; -.
DR BioGRID-ORCS; 74347; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Tldc1; mouse.
DR PRO; PR:Q8K0P3; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8K0P3; protein.
DR Bgee; ENSMUSG00000034105; Expressed in retinal neural layer and 166 other tissues.
DR ExpressionAtlas; Q8K0P3; baseline and differential.
DR Genevisible; Q8K0P3; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005765; C:lysosomal membrane; ISS:UniProtKB.
DR GO; GO:0016020; C:membrane; ISS:UniProtKB.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:1903204; P:negative regulation of oxidative stress-induced neuron death; IDA:MGI.
DR GO; GO:0150032; P:positive regulation of protein localization to lysosome; ISS:UniProtKB.
DR GO; GO:0030334; P:regulation of cell migration; ISS:UniProtKB.
DR GO; GO:0042127; P:regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0043200; P:response to amino acid; ISS:UniProtKB.
DR GO; GO:0032868; P:response to insulin; ISS:UniProtKB.
DR GO; GO:0031667; P:response to nutrient levels; ISS:UniProtKB.
DR GO; GO:0031929; P:TOR signaling; ISS:UniProtKB.
DR InterPro; IPR006571; TLDc_dom.
DR Pfam; PF07534; TLD; 1.
DR SMART; SM00584; TLDc; 1.
DR PROSITE; PS51886; TLDC; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Lipoprotein; Lysosome; Membrane;
KW Myristate; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..455
FT /note="MTOR-associated protein MEAK7"
FT /id="PRO_0000313641"
FT DOMAIN 242..410
FT /note="TLDc"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01234"
FT REGION 435..455
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 436..455
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250|UniProtKB:Q6P9B6"
FT VAR_SEQ 21..72
FT /note="QAEVDKLFDVLSSSEGGVATGTFSLEAMKSHVKEALPPAMVTRLYNGMQRVK
FT -> HPGCAVRHLPQLPPGPGASATLAPAVLHAAPRAELLPALQPHHQPGAQPACP (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15368895"
FT /id="VSP_030079"
FT VAR_SEQ 73..455
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15368895"
FT /id="VSP_030080"
SQ SEQUENCE 455 AA; 50845 MW; 470FAB93926646C0 CRC64;
MGNAKSLSGQ KMASRFLPEE QAEVDKLFDV LSSSEGGVAT GTFSLEAMKS HVKEALPPAM
VTRLYNGMQR VKPTDRTLGS CRSVSREQFT AFLSQLLRGS CEEKGLMVMN MISDAEGPTK
TRDVQKFTED LVASVAHVLT HRHELRGWTC RKSEVPPDSM QAMVAQLLSE MKFQDGYKFQ
GPQCLDQVCD QAMIEEWVFH VPHVGMFLSV VVHRGLCLLG SSFDPSTLVP ECLADQGGRF
ESILDVLSVI YLSSHLAPEH RQRWRLLFST QLHGQSFSQL CSHITSQGPS LLVLEDRDGY
VFGGFASCSW EVKPQFQGDN RCFLFSIAPR MATHLHTGYN NHFMYLNYGQ QTMPNGLGMG
GQHHYFGLWV AADFGKGHSK AKPACTTYNS PQLSAQEDFL FDKMEVWGLG NLLEEYEGKN
KKSVLDSNPE ARSLLEISGR ARHSEGLREV PRDED
