MEB2_ARATH
ID MEB2_ARATH Reviewed; 550 AA.
AC F4KFS7; B9DH25; Q8LCU6; Q9FNF3;
DT 01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=Membrane protein of ER body 2;
GN Name=MEB2; OrderedLocusNames=At5g24290; ORFNames=MOP9.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia; TISSUE=Root;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, INDUCTION, DISRUPTION PHENOTYPE, AND
RP INTERACTION WITH NAI2.
RC STRAIN=cv. Columbia;
RX PubMed=23166355; DOI=10.1104/pp.112.207654;
RA Yamada K., Nagano A.J., Nishina M., Hara-Nishimura I., Nishimura M.;
RT "Identification of two novel endoplasmic reticulum body-specific integral
RT membrane proteins.";
RL Plant Physiol. 161:108-120(2013).
CC -!- FUNCTION: May sequester excess cytosolic iron and manganese into
CC endoplasmic reticulum to reduce metal ion toxicity. Not essential for
CC the accumulation of ER body components, including PYK10.
CC {ECO:0000269|PubMed:23166355}.
CC -!- SUBUNIT: Interacts directly or indirectly with NAI2.
CC {ECO:0000269|PubMed:23166355}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:23166355}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:23166355}. Note=Located in ER bodies.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4KFS7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4KFS7-2; Sequence=VSP_056762;
CC -!- INDUCTION: Induced by NAI1. {ECO:0000269|PubMed:23166355}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype and no effect on pathogen
CC sensitivity. {ECO:0000269|PubMed:23166355}.
CC -!- SIMILARITY: Belongs to the CCC1 family. {ECO:0000305}.
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DR EMBL; AB006701; BAB10395.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93280.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93281.1; -; Genomic_DNA.
DR EMBL; AY086399; AAM64466.1; -; mRNA.
DR EMBL; BT008326; AAP37685.1; -; mRNA.
DR EMBL; AK228214; BAF00167.1; -; mRNA.
DR EMBL; AK317370; BAH20042.1; -; mRNA.
DR RefSeq; NP_568441.2; NM_122335.4. [F4KFS7-2]
DR RefSeq; NP_851067.1; NM_180736.3. [F4KFS7-1]
DR AlphaFoldDB; F4KFS7; -.
DR BioGRID; 17771; 3.
DR IntAct; F4KFS7; 3.
DR STRING; 3702.AT5G24290.1; -.
DR iPTMnet; F4KFS7; -.
DR PaxDb; F4KFS7; -.
DR PRIDE; F4KFS7; -.
DR ProteomicsDB; 250840; -. [F4KFS7-1]
DR EnsemblPlants; AT5G24290.1; AT5G24290.1; AT5G24290. [F4KFS7-1]
DR EnsemblPlants; AT5G24290.2; AT5G24290.2; AT5G24290. [F4KFS7-2]
DR GeneID; 832496; -.
DR Gramene; AT5G24290.1; AT5G24290.1; AT5G24290. [F4KFS7-1]
DR Gramene; AT5G24290.2; AT5G24290.2; AT5G24290. [F4KFS7-2]
DR KEGG; ath:AT5G24290; -.
DR Araport; AT5G24290; -.
DR TAIR; locus:2169804; AT5G24290.
DR eggNOG; ENOG502QQ85; Eukaryota.
DR InParanoid; F4KFS7; -.
DR OMA; IFTCLAC; -.
DR OrthoDB; 953616at2759; -.
DR PRO; PR:F4KFS7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4KFS7; baseline and differential.
DR Genevisible; F4KFS7; AT.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0010168; C:ER body; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005381; F:iron ion transmembrane transporter activity; IGI:TAIR.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IGI:TAIR.
DR GO; GO:0030026; P:cellular manganese ion homeostasis; IBA:GO_Central.
DR GO; GO:0006880; P:intracellular sequestering of iron ion; IBA:GO_Central.
DR InterPro; IPR008217; Ccc1_fam.
DR PANTHER; PTHR31851; PTHR31851; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Endoplasmic reticulum; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..550
FT /note="Membrane protein of ER body 2"
FT /id="PRO_0000430468"
FT TRANSMEM 374..394
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 458..478
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 500..520
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT REGION 46..199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 134..165
FT /evidence="ECO:0000255"
FT COILED 393..418
FT /evidence="ECO:0000255"
FT COMPBIAS 71..135
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 178..195
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 77..92
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172, ECO:0000303|Ref.5"
FT /id="VSP_056762"
FT CONFLICT 91
FT /note="D -> G (in Ref. 6; BAH20042)"
FT /evidence="ECO:0000305"
FT CONFLICT 202
FT /note="L -> F (in Ref. 3; AAM64466)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 550 AA; 61046 MW; 9F6E6C92E8FA2358 CRC64;
MEKSNQPVHV TLSELKDGDK EIVDAEFLVD LLESYRFGKD NVPAREFRSK AAATAPAPVN
TTEIELEEDN DGSQAQGNNS VSESTSSLFS DSDPIVLEST VSETGSNEES ETGSNEENGN
NWLESSSTNL PNVENKRQRN GEDCEIEEEE ENNERSLSDS EEKSNLEKLL GTQENYELGN
EDEEKNERSS SDSEEKSNLE NLLATQENYE LYCPSCSTCI TRNVVLKKRK RGKHVNSSLD
LKPDIPVVEP DEPSDIEEME SPVKVYVPET RIEDDQEDKE GTIFTCLVCD LKYFIRLGTK
FLQLDYIRGK PVEKSVEEYI DVRKSINTTQ SPPQIQPDGE RFAIELLKST VYGGLTETIT
SLGVVSSASA SGSSTMNILA LAVANLAGGL IVLAQNFQDL RNSSDQEKDR YEELLGRRTK
SRIHILVAVM SYIFFGLIPP LVYAFSFYET GIKNYKLISV FLGSLVCVIL LGSIKVYVRK
PTNSCGSTKA YLKSAAYYTS IVVASCGISY VVGDIMGEYI EKLSLVGLDQ ISITSPCYGI
KPEECRFTSF
