MEBL_ARATH
ID MEBL_ARATH Reviewed; 761 AA.
AC Q8LPT3; Q93ZP6; Q9STN9;
DT 01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Membrane protein of ER body-like protein;
GN Name=MEBL; OrderedLocusNames=At4g27870; ORFNames=T27E11.110;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION, FUNCTION, AND INDUCTION.
RC STRAIN=cv. Columbia;
RX PubMed=23166355; DOI=10.1104/pp.112.207654;
RA Yamada K., Nagano A.J., Nishina M., Hara-Nishimura I., Nishimura M.;
RT "Identification of two novel endoplasmic reticulum body-specific integral
RT membrane proteins.";
RL Plant Physiol. 161:108-120(2013).
CC -!- FUNCTION: Not essential for the accumulation of ER body components,
CC including PYK10. {ECO:0000269|PubMed:23166355}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- INDUCTION: Not induced by NAI1. {ECO:0000269|PubMed:23166355}.
CC -!- SIMILARITY: Belongs to the CCC1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL08260.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAB43975.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB81436.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL078579; CAB43975.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161571; CAB81436.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85403.1; -; Genomic_DNA.
DR EMBL; AY094391; AAM19770.1; -; mRNA.
DR EMBL; BT002626; AAO11542.1; -; mRNA.
DR EMBL; AY056404; AAL08260.1; ALT_FRAME; mRNA.
DR PIR; T09026; T09026.
DR RefSeq; NP_567789.2; NM_118925.3.
DR AlphaFoldDB; Q8LPT3; -.
DR BioGRID; 14187; 1.
DR IntAct; Q8LPT3; 1.
DR STRING; 3702.AT4G27870.1; -.
DR iPTMnet; Q8LPT3; -.
DR SwissPalm; Q8LPT3; -.
DR PaxDb; Q8LPT3; -.
DR PRIDE; Q8LPT3; -.
DR EnsemblPlants; AT4G27870.1; AT4G27870.1; AT4G27870.
DR GeneID; 828900; -.
DR Gramene; AT4G27870.1; AT4G27870.1; AT4G27870.
DR KEGG; ath:AT4G27870; -.
DR Araport; AT4G27870; -.
DR TAIR; locus:2137390; AT4G27870.
DR eggNOG; ENOG502QQ85; Eukaryota.
DR HOGENOM; CLU_429849_0_0_1; -.
DR InParanoid; Q8LPT3; -.
DR PhylomeDB; Q8LPT3; -.
DR PRO; PR:Q8LPT3; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8LPT3; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0010168; C:ER body; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0005381; F:iron ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0030026; P:cellular manganese ion homeostasis; IBA:GO_Central.
DR GO; GO:0006880; P:intracellular sequestering of iron ion; IBA:GO_Central.
DR InterPro; IPR008217; Ccc1_fam.
DR PANTHER; PTHR31851; PTHR31851; 2.
PE 2: Evidence at transcript level;
KW Coiled coil; Endoplasmic reticulum; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..761
FT /note="Membrane protein of ER body-like protein"
FT /id="PRO_0000430469"
FT TRANSMEM 549..569
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 573..593
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 640..660
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 670..690
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 702..722
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT REGION 1..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 120..162
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 338..374
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 416..448
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 186..206
FT /evidence="ECO:0000255"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 18..46
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 47..69
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 137..151
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 418..432
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 433..448
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 761 AA; 82551 MW; CFF6DA64E8A5BD70 CRC64;
MGSAAELTEP TTGTEKLHQP IEVEEDDEQI VDLERKTFRH GKGHDTSVDS STITNTSSSS
SSSFSGDGGT EETPDFHSNG DGEHTDLVNL EVPELETEIV FHQEHDDGKN CIVFFDGEQG
SESAGAIPDA HEESQTADLN GEQTQLEPEN GSTSEDNERS REIEEVLDGD VSKDLDAVDP
LAGEVIEEEV DFEDVEYHDV ENMMDKQETH DLYCPNCDSC ITKKVILKRR KRKIRRHELG
DSKRPHLTEP LFHSEDNLPS LDGGENSANE SFVFKCLSCF TIFIPKGVSS KPIPPRQGVE
GLKIQPNPQV EATGDSNWFS SIFGLNKKES AIQQGGASSS VLEANPPPRE SIVPVVNPSR
GNLSPMRKDT TGSAVVQPDA ATSIQVAKSN DTSEIVNNGA IVGDGQKFLA PMVEEQTQQK
IDNDDSSTAD GNHTSDKGRL SPIQPSHGMS ILNTVTNGPD GLKVETTIHE EGAPLLFEGK
DTPDTSTADF GLTKVTGVMD TGDRGVITGP ANPEIDISAG NLLEEGSLRE PLMRRVVVQG
RKLEILKSIV YGGLLEAITS LGVISSAAGS GASMLNILVL GLANLLGGLI LIIHNLQELR
EEEPIRTTTE DNQTNGREEE EGRYKRLLGR RENFTLHATV AILSFIITGI LPPVVYYFSF
SEKHNKDYKV ASVFGASLFC IVLLAIAKAH VRYPRGSYLK SILYYGSIAV SVSGISYVVG
NFLEQLLEKH GWSDGSETPV GQMMLSSLMG RKAGFGYSSS Y
