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MEC10_CAEEL
ID   MEC10_CAEEL             Reviewed;         724 AA.
AC   P34886; P37087;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 143.
DE   RecName: Full=Degenerin mec-10;
DE   AltName: Full=Mechanosensory abnormality protein 10;
GN   Name=mec-10 {ECO:0000312|WormBase:F16F9.5};
GN   ORFNames=F16F9.5 {ECO:0000312|WormBase:F16F9.5};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, AND MUTAGENESIS OF SER-105
RP   AND ALA-673.
RC   STRAIN=Bristol N2;
RX   PubMed=7509039; DOI=10.1038/367467a0;
RA   Huang M., Chalfie M.;
RT   "Gene interactions affecting mechanosensory transduction in Caenorhabditis
RT   elegans.";
RL   Nature 367:467-470(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [3]
RP   FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX   PubMed=11875573; DOI=10.1038/4151039a;
RA   Goodman M.B., Ernstrom G.G., Chelur D.S., O'Hagan R., Yao C.A., Chalfie M.;
RT   "MEC-2 regulates C. elegans DEG/ENaC channels needed for
RT   mechanosensation.";
RL   Nature 415:1039-1042(2002).
RN   [4]
RP   FUNCTION, IDENTIFICATION IN THE DEGENERIN CHANNEL COMPLEX, AND INTERACTION
RP   WITH MEC-4 AND MEC-6.
RX   PubMed=12478294; DOI=10.1038/nature01205;
RA   Chelur D.S., Ernstrom G.G., Goodman M.B., Yao C.A., Chen L., O'Hagan R.,
RA   Chalfie M.;
RT   "The mechanosensory protein MEC-6 is a subunit of the C. elegans touch-cell
RT   degenerin channel.";
RL   Nature 420:669-673(2002).
RN   [5]
RP   FUNCTION, AND MUTAGENESIS OF ALA-673.
RX   PubMed=17261841; DOI=10.1085/jgp.200609672;
RA   Brown A.L., Fernandez-Illescas S.M., Liao Z., Goodman M.B.;
RT   "Gain-of-function mutations in the MEC-4 DEG/ENaC sensory
RT   mechanotransduction channel alter gating and drug blockade.";
RL   J. Gen. Physiol. 129:161-173(2007).
RN   [6]
RP   FUNCTION, AND MUTAGENESIS OF SER-105.
RX   PubMed=17611271; DOI=10.1523/jneurosci.1405-07.2007;
RA   Liu T., Kim K., Li C., Barr M.M.;
RT   "FMRFamide-like neuropeptides and mechanosensory touch receptor neurons
RT   regulate male sexual turning behavior in Caenorhabditis elegans.";
RL   J. Neurosci. 27:7174-7182(2007).
CC   -!- FUNCTION: Subunit of an amiloride-sensitive cation channel (degenerin
CC       channel complex) permeable for sodium, potassium, lithium and N-
CC       methylglucamine, and required for mechanosensory transduction (touch
CC       sensitivity) (PubMed:11875573, PubMed:17261841, PubMed:7509039,
CC       PubMed:12478294). Negatively regulates the turning step of male mating
CC       behavior (PubMed:17611271). {ECO:0000269|PubMed:11875573,
CC       ECO:0000269|PubMed:12478294, ECO:0000269|PubMed:17261841,
CC       ECO:0000269|PubMed:17611271, ECO:0000269|PubMed:7509039}.
CC   -!- SUBUNIT: Component of a non-voltage-gated amiloride-sensitive cation
CC       channel complex (also called the degenerin channel complex) composed of
CC       at least the mec-2, mec-4, mec-6 and mec-10 subunits; the complex
CC       mediates mechanotransduction in touch cells (PubMed:7509039,
CC       PubMed:11875573, PubMed:12478294). Interacts with mec-4 and mec-6
CC       (PubMed:12478294). {ECO:0000269|PubMed:11875573,
CC       ECO:0000269|PubMed:12478294, ECO:0000269|PubMed:7509039}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11875573};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:11875573}.
CC   -!- SIMILARITY: Belongs to the amiloride-sensitive sodium channel (TC
CC       1.A.6) family. {ECO:0000305}.
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DR   EMBL; L25312; AAA17404.1; -; mRNA.
DR   EMBL; BX284606; CCD69038.1; -; Genomic_DNA.
DR   PIR; T25700; T25700.
DR   RefSeq; NP_509438.1; NM_077037.4.
DR   AlphaFoldDB; P34886; -.
DR   SMR; P34886; -.
DR   BioGRID; 46022; 3.
DR   STRING; 6239.F16F9.5; -.
DR   TCDB; 1.A.6.2.2; the epithelial na(+) channel (enac) family.
DR   PaxDb; P34886; -.
DR   EnsemblMetazoa; F16F9.5.1; F16F9.5.1; WBGene00003174.
DR   GeneID; 181101; -.
DR   KEGG; cel:CELE_F16F9.5; -.
DR   UCSC; F16F9.5; c. elegans.
DR   CTD; 181101; -.
DR   WormBase; F16F9.5; CE09444; WBGene00003174; mec-10.
DR   eggNOG; KOG4294; Eukaryota.
DR   GeneTree; ENSGT00970000196327; -.
DR   HOGENOM; CLU_017673_0_0_1; -.
DR   InParanoid; P34886; -.
DR   OMA; MSQAKHN; -.
DR   OrthoDB; 686369at2759; -.
DR   PhylomeDB; P34886; -.
DR   PRO; PR:P34886; -.
DR   Proteomes; UP000001940; Chromosome X.
DR   Bgee; WBGene00003174; Expressed in larva and 1 other tissue.
DR   GO; GO:0044298; C:cell body membrane; IDA:UniProtKB.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0015280; F:ligand-gated sodium channel activity; IDA:WormBase.
DR   GO; GO:0050976; P:detection of mechanical stimulus involved in sensory perception of touch; IGI:UniProtKB.
DR   GO; GO:0007638; P:mechanosensory behavior; IMP:WormBase.
DR   GO; GO:1905789; P:positive regulation of detection of mechanical stimulus involved in sensory perception of touch; IGI:UniProtKB.
DR   GO; GO:1905792; P:positive regulation of mechanosensory behavior; IGI:UniProtKB.
DR   GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0009612; P:response to mechanical stimulus; IMP:WormBase.
DR   GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR   InterPro; IPR004726; Deg-1.
DR   InterPro; IPR001873; ENaC.
DR   InterPro; IPR020903; ENaC_CS.
DR   PANTHER; PTHR11690; PTHR11690; 1.
DR   Pfam; PF00858; ASC; 1.
DR   PRINTS; PR01078; AMINACHANNEL.
DR   TIGRFAMs; TIGR00867; deg-1; 1.
DR   PROSITE; PS01206; ASC; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Glycoprotein; Ion channel; Ion transport; Membrane;
KW   Potassium; Potassium transport; Reference proteome; Sensory transduction;
KW   Sodium; Sodium channel; Sodium transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..724
FT                   /note="Degenerin mec-10"
FT                   /id="PRO_0000181288"
FT   TOPO_DOM        1..122
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        123..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        144..684
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        685..705
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        706..724
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          229..265
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..15
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        293
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        369
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        463
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        605
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        624
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         105
FT                   /note="S->F: In e1515; loss of mechanosensory transduction
FT                   between touch cells. Abnormal repetitive turning behavior
FT                   during male mating."
FT                   /evidence="ECO:0000269|PubMed:17611271,
FT                   ECO:0000269|PubMed:7509039"
FT   MUTAGEN         673
FT                   /note="A->C: Slight increase in channel current."
FT                   /evidence="ECO:0000269|PubMed:17261841,
FT                   ECO:0000269|PubMed:7509039"
FT   MUTAGEN         673
FT                   /note="A->D: Increased channel current."
FT                   /evidence="ECO:0000269|PubMed:17261841,
FT                   ECO:0000269|PubMed:7509039"
FT   MUTAGEN         673
FT                   /note="A->V: Causes degeneration of the PLM mechanosensory
FT                   neuron and increases channel current."
FT                   /evidence="ECO:0000269|PubMed:17261841,
FT                   ECO:0000269|PubMed:7509039"
SQ   SEQUENCE   724 AA;  82382 MW;  196819763C2CBA57 CRC64;
     MNRNPRMSKF QPNPRSRSRF QDETDLRSLR SFKTDFSNYL ASDTNFLNVA EIMTSYAYGE
     SNNAHEKEIQ CDLLTENGGI EIDPTRLSYR ERIRWHLQQF CYKTSSHGIP MLGQAPNSLY
     RAAWVFLLLI CAIQFINQAV AVIQKYQKMD KITDIQLKFD TAPFPAITLC NLNPYKDSVI
     RSHDSISKIL GVFKSVMKKA GDSSSEALEE EEETEYDMNG ITIQAKRKKR GAGEKGTFEP
     ANSACECDEE DGSNECEERS TEKPSGDNDM CICAFDRQTN DAWPCHRKEQ WTNTTCQTCD
     EHYLCSKKAK KGTKRSELKK EPCICESKGL FCIKHEHAAM VLNLWEYFGD SEDFSEISTE
     EREALGFGNM TDEVAIVTKA KENIIFAMSA LSEEQRILMS QAKHNLIHKC SFNGKPCDID
     QDFELVADPT FGNCFVFNHD REIFKSSVRA GPQYGLRVML FVNASDYLPT SEAVGIRLTI
     HDKDDFPFPD TFGYSAPTGY ISSFGMRMKK MSRLPAPYGD CVEDGATSNY IYKGYAYSTE
     GCYRTCFQEL IIDRCGCSDP RFPSIGGVQP CQVFNKNHRE CLEKHTHQIG EIHGSFKCRC
     QQPCNQTIYT TSYSEAIWPS QALNISLGQC EKEAEECNEE YKENAAMLEV FYEALNFEVL
     SESEAYGIVK MMADFGGHLG LWSGVSVMTC CEFVCLAFEL IYMAIAHHIN QQRIRRRENA
     ANEY
 
 
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