MEC15_CAEEL
ID MEC15_CAEEL Reviewed; 406 AA.
AC Q22071;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=F-box/WD repeat-containing protein mec-15 {ECO:0000305};
DE AltName: Full=Mechanosensory abnormality protein 15 {ECO:0000312|WormBase:T01E8.4};
GN Name=mec-15 {ECO:0000312|WormBase:T01E8.4};
GN ORFNames=T01E8.4 {ECO:0000312|WormBase:T01E8.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=8692859; DOI=10.1073/pnas.93.13.6577;
RA Gu G., Caldwell G.A., Chalfie M.;
RT "Genetic interactions affecting touch sensitivity in Caenorhabditis
RT elegans.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:6577-6582(1996).
RN [3] {ECO:0000305}
RP INTERACTION WITH SKR-1.
RX PubMed=11864566; DOI=10.1016/s0960-9822(02)00657-7;
RA Yamanaka A., Yada M., Imaki H., Koga M., Ohshima Y., Nakayama K.;
RT "Multiple Skp1-related proteins in Caenorhabditis elegans: diverse patterns
RT of interaction with Cullins and F-box proteins.";
RL Curr. Biol. 12:267-275(2002).
RN [4] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=19652181; DOI=10.1534/genetics.109.105726;
RA Bounoutas A., Zheng Q., Nonet M.L., Chalfie M.;
RT "mec-15 encodes an F-box protein required for touch receptor neuron
RT mechanosensation, synapse formation and development.";
RL Genetics 183:607-617(2009).
RN [5] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=23527112; DOI=10.1371/journal.pone.0059132;
RA Sun Y., Hu Z., Goeb Y., Dreier L.;
RT "The F-box protein MEC-15 (FBXW9) promotes synaptic transmission in
RT GABAergic motor neurons in C. elegans.";
RL PLoS ONE 8:E59132-E59132(2013).
CC -!- FUNCTION: Plays a role in mechanosensory transduction (touch
CC sensitivity), touch receptor neuron development and synapse formation
CC (PubMed:8692859, PubMed:19652181). Regulates expression of the protein
CC snb-1 and the distribution of synaptic vesicles at synapses to promote
CC synaptic transmission at the neuromuscular junctions of GABAergic motor
CC neurons (PubMed:23527112). {ECO:0000269|PubMed:19652181,
CC ECO:0000269|PubMed:23527112, ECO:0000269|PubMed:8692859}.
CC -!- SUBUNIT: May interact with the SCF ubiquitin ligase complex component
CC skr-1. {ECO:0000269|PubMed:11864566}.
CC -!- SUBCELLULAR LOCATION: Perikaryon {ECO:0000269|PubMed:23527112}.
CC -!- TISSUE SPECIFICITY: Expressed in several neurons in the head, tail and
CC ventral cord, but absent in touch receptor neurons in adults
CC (PubMed:19652181, PubMed:23527112). Expressed in GABAergic and
CC cholinergic motor neurons (PubMed:23527112).
CC {ECO:0000269|PubMed:19652181, ECO:0000269|PubMed:23527112}.
CC -!- DEVELOPMENTAL STAGE: Expressed in touch receptor neurons and several
CC neurons in the head, tail and ventral cord during larval development.
CC {ECO:0000269|PubMed:19652181}.
CC -!- DISRUPTION PHENOTYPE: Temperature-sensitive phenotype with defective
CC touch receptor neuron synaptic transmission conferring variable degrees
CC of touch insensitivity amongst individual animals (PubMed:8692859,
CC PubMed:19652181). Touch receptor neurons and GABAergic motor neurons
CC have mis-localized presynaptic vesicles as indicated by aberrant
CC expression of the protein snb-1 and distorted cell bodies
CC (PubMed:19652181, PubMed:23527112). Reduced rate of inhibitory
CC postsynaptic currents at the neuromuscular junctions of GABAergic motor
CC neurons (PubMed:23527112). No effects on cholinergic synaptic
CC transmission or snb-1 distribution in cholinergic motor neurons
CC (PubMed:23527112). Increased rate of paralysis, in response to
CC acetylcholine esterase inhibition by the drug Aldicarb, due to
CC increased accumulation of acetylcholine in the extracellular fluid
CC resulting permanent contraction of body wall muscles (PubMed:23527112).
CC Knockout with a mec-7 heterozygous mutant, but not a null mec-7 mutant,
CC rescues the touch sensitivity defect in the mec-15 single mutant
CC (PubMed:19652181). {ECO:0000269|PubMed:19652181,
CC ECO:0000269|PubMed:23527112, ECO:0000269|PubMed:8692859}.
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DR EMBL; BX284602; CAA88747.1; -; Genomic_DNA.
DR PIR; T24301; T24301.
DR RefSeq; NP_496207.1; NM_063806.6.
DR AlphaFoldDB; Q22071; -.
DR SMR; Q22071; -.
DR STRING; 6239.T01E8.4; -.
DR EPD; Q22071; -.
DR PaxDb; Q22071; -.
DR PeptideAtlas; Q22071; -.
DR EnsemblMetazoa; T01E8.4.1; T01E8.4.1; WBGene00003177.
DR GeneID; 174587; -.
DR KEGG; cel:CELE_T01E8.4; -.
DR UCSC; T01E8.4; c. elegans.
DR CTD; 174587; -.
DR WormBase; T01E8.4; CE02308; WBGene00003177; mec-15.
DR eggNOG; KOG0274; Eukaryota.
DR GeneTree; ENSGT00940000173961; -.
DR HOGENOM; CLU_064154_0_0_1; -.
DR InParanoid; Q22071; -.
DR OMA; MSYSGCE; -.
DR OrthoDB; 1109214at2759; -.
DR PhylomeDB; Q22071; -.
DR Reactome; R-CEL-8951664; Neddylation.
DR Reactome; R-CEL-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR PRO; PR:Q22071; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00003177; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0043204; C:perikaryon; IDA:UniProtKB.
DR GO; GO:0098793; C:presynapse; IEA:GOC.
DR GO; GO:0050976; P:detection of mechanical stimulus involved in sensory perception of touch; IGI:UniProtKB.
DR GO; GO:0007638; P:mechanosensory behavior; IGI:UniProtKB.
DR GO; GO:0048666; P:neuron development; IMP:WormBase.
DR GO; GO:1905789; P:positive regulation of detection of mechanical stimulus involved in sensory perception of touch; IGI:UniProtKB.
DR GO; GO:0097151; P:positive regulation of inhibitory postsynaptic potential; IMP:UniProtKB.
DR GO; GO:1905792; P:positive regulation of mechanosensory behavior; IGI:UniProtKB.
DR GO; GO:0045933; P:positive regulation of muscle contraction; IMP:UniProtKB.
DR GO; GO:0032230; P:positive regulation of synaptic transmission, GABAergic; IMP:UniProtKB.
DR GO; GO:1902805; P:positive regulation of synaptic vesicle transport; IMP:WormBase.
DR GO; GO:0016080; P:synaptic vesicle targeting; IMP:UniProtKB.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF12937; F-box-like; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00256; FBOX; 1.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..406
FT /note="F-box/WD repeat-containing protein mec-15"
FT /evidence="ECO:0000305"
FT /id="PRO_0000442206"
FT DOMAIN 6..53
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REPEAT 101..142
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 156..195
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 242..279
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 281..320
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 365..406
FT /note="WD 5"
FT /evidence="ECO:0000255"
SQ SEQUENCE 406 AA; 46173 MW; D3B5F68B370C6F0C CRC64;
MTNAQPTELI SLPSELLCHL FTYLPQRQLI TEIPLVCRRF NTILNDDKFW SRRIRTEQKV
RLPDCELKHP EYEPKKSFYA MHRQRDRWRA WETQTVITAP GHSATVDSVM LFENNNKQFC
LSGSRDRTIR LWSIENARNG EDTEQNPWTV AKDDTAHSGW IWNMAQESTS TNFYTTSWDS
TVKSWHITDN GALQNLNSVN VGSAAQCISC SGNENEVVCT TFAKRVAVID SRTFQVVADH
KLHKRAVIAL AVQGDKIFTS GEDRLMMMVD RRNFSKPVLF EYSPTAYKSC LSLQCNQLLT
STSDGKVKLY DANNFNVLQT YSVGSYTRQS FLEHGAHLIM ARSLKRNYTF SIYSPGTRSQ
QWCASHELAA EPAKFDYSPS SRTLAIGNGD SSILFCLPAS APDQEN
