ID   MEC3_CAEEL              Reviewed;         321 AA.
AC   P09088; B3GWD3; O02240; Q7YTQ4;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 2.
DT   03-AUG-2022, entry version 185.
DE   RecName: Full=Mechanosensory protein 3;
GN   Name=mec-3; ORFNames=F01D4.6;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), AND FUNCTION.
RX   PubMed=2898300; DOI=10.1016/0092-8674(88)90174-2;
RA   Way J.C., Chalfie M.;
RT   "mec-3, a homeobox-containing gene that specifies differentiation of the
RT   touch receptor neurons in C. elegans.";
RL   Cell 54:5-16(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION, AND FUNCTION.
RX   PubMed=1361171; DOI=10.1002/j.1460-2075.1992.tb05604.x;
RA   Xue D., Finney M., Ruvkun G., Chalfie M.;
RT   "Regulation of the mec-3 gene by the C.elegans homeoproteins UNC-86 and
RT   MEC-3.";
RL   EMBO J. 11:4969-4979(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [4]
RP   FUNCTION, AND MUTAGENESIS OF 69-TRP--ASP-321.
RX   PubMed=29031632; DOI=10.1016/j.ydbio.2017.10.009;
RA   O'Brien B.M.J., Palumbos S.D., Novakovic M., Shang X., Sundararajan L.,
RA   Miller D.M. III;
RT   "Separate transcriptionally regulated pathways specify distinct classes of
RT   sister dendrites in a nociceptive neuron.";
RL   Dev. Biol. 432:248-257(2017).
CC   -!- FUNCTION: Specifies differentiation of the set of six touch receptor
CC       neurons (PubMed:2898300). Promotes outgrowth of lateral dendritic
CC       branches on the PVD nociceptive neurons, probably acting both directly,
CC       and upstream of zinc finger protein egl-46 (PubMed:29031632). Binds
CC       cooperatively as a heterodimer with unc-86 to sites in the mec-3 gene
CC       promoter (PubMed:1361171). {ECO:0000269|PubMed:1361171,
CC       ECO:0000269|PubMed:2898300, ECO:0000269|PubMed:29031632}.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=a {ECO:0000312|WormBase:F01D4.6a};
CC         IsoId=P09088-1; Sequence=Displayed;
CC       Name=c {ECO:0000312|WormBase:F01D4.6c};
CC         IsoId=P09088-3; Sequence=VSP_058152;
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DR   EMBL; L02877; AAA50614.1; -; mRNA.
DR   EMBL; M20244; AAA28108.1; -; Genomic_DNA.
DR   EMBL; Z81054; CAB02885.3; -; Genomic_DNA.
DR   EMBL; BX284604; CAQ58102.1; -; Genomic_DNA.
DR   PIR; S28390; S28390.
DR   PIR; T20458; T20458.
DR   RefSeq; NP_001023111.1; NM_001027940.3. [P09088-1]
DR   RefSeq; NP_001129852.1; NM_001136380.2. [P09088-3]
DR   AlphaFoldDB; P09088; -.
DR   SMR; P09088; -.
DR   BioGRID; 43040; 83.
DR   IntAct; P09088; 77.
DR   STRING; 6239.F01D4.6a; -.
DR   PaxDb; P09088; -.
DR   EnsemblMetazoa; F01D4.6a.1; F01D4.6a.1; WBGene00003167. [P09088-1]
DR   EnsemblMetazoa; F01D4.6c.1; F01D4.6c.1; WBGene00003167. [P09088-3]
DR   GeneID; 177938; -.
DR   UCSC; F01D4.6a; c. elegans. [P09088-1]
DR   CTD; 177938; -.
DR   WormBase; F01D4.6a; CE27422; WBGene00003167; mec-3. [P09088-1]
DR   WormBase; F01D4.6c; CE42646; WBGene00003167; mec-3. [P09088-3]
DR   eggNOG; KOG0490; Eukaryota.
DR   GeneTree; ENSGT00940000164085; -.
DR   InParanoid; P09088; -.
DR   OMA; SYHENCV; -.
DR   OrthoDB; 1014909at2759; -.
DR   PhylomeDB; P09088; -.
DR   SignaLink; P09088; -.
DR   PRO; PR:P09088; -.
DR   Proteomes; UP000001940; Chromosome IV.
DR   Bgee; WBGene00003167; Expressed in larva and 3 other tissues.
DR   ExpressionAtlas; P09088; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IDA:WormBase.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:WormBase.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR   GO; GO:0008134; F:transcription factor binding; IPI:WormBase.
DR   GO; GO:0007638; P:mechanosensory behavior; IMP:WormBase.
DR   GO; GO:1903026; P:negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding; IDA:WormBase.
DR   GO; GO:0030182; P:neuron differentiation; IMP:WormBase.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:WormBase.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0009612; P:response to mechanical stimulus; IMP:WormBase.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR001781; Znf_LIM.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00412; LIM; 2.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00132; LIM; 2.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR   PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE   1: Evidence at protein level;
KW   Alternative splicing; Developmental protein; DNA-binding; Homeobox;
KW   LIM domain; Metal-binding; Nucleus; Reference proteome; Repeat; Zinc.
FT   CHAIN           1..321
FT                   /note="Mechanosensory protein 3"
FT                   /id="PRO_0000075840"
FT   DOMAIN          27..86
FT                   /note="LIM zinc-binding 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DOMAIN          87..152
FT                   /note="LIM zinc-binding 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DNA_BIND        217..276
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   VAR_SEQ         1..43
FT                   /note="Missing (in isoform c)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_058152"
FT   MUTAGEN         69..321
FT                   /note="Missing: In e1338; PVD neurons have severe dendritic
FT                   branching defects, and significantly longer axonal
FT                   projection in the ventral nerve cord. On either egl-44 or
FT                   egl-46 mutant backgrounds, abolishes expression of tyramine
FT                   receptor ser-2."
FT                   /evidence="ECO:0000269|PubMed:29031632"
SQ   SEQUENCE   321 AA;  37088 MW;  9231E7C3F5054853 CRC64;
     MEMLESKPLS AISMVIDSIG VDHENQNKCN CCNEQIYDRY IYRMDNRSYH ENCVKCTICE
     SPLAEKCFWK NGRIYCSQHY YKDHSIHRCA GCKKGVSPTD MVYKLKAGLV FHVECHCCSL
     CGRHLSPGEQ ILVDDTMMTV SCMSHYPPQM DDNAPGAIGS AVDIPSCSTE NPIAPYPIDE
     SFSSAFQVKK EVDAYGYNFE HYSFSDFCDD DSRMLKRRGP RTTIKQNQLD VLNEMFSNTP
     KPSKHARAKL ALETGLSMRV IQVWFQNRRS KERRLKHLCN YLRHYEQRGL IPPPIHFRNE
     EMDTTDFNAF CGNFEEEDDE D