MEC6_CAEEL
ID MEC6_CAEEL Reviewed; 377 AA.
AC O01811; Q8T7S8;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Mechanosensory abnormality protein 6;
GN Name=mec-6 {ECO:0000312|WormBase:W02D3.3};
GN ORFNames=W02D3.3 {ECO:0000312|WormBase:W02D3.3};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, IDENTIFICATION IN THE DEGENERIN CHANNEL COMPLEX, INTERACTION
RP WITH MEC-2; MEC-4 AND MEC-10, GLYCOSYLATION, AND MUTAGENESIS OF GLY-235.
RX PubMed=12478294; DOI=10.1038/nature01205;
RA Chelur D.S., Ernstrom G.G., Goodman M.B., Yao C.A., Chen L., O'Hagan R.,
RA Chalfie M.;
RT "The mechanosensory protein MEC-6 is a subunit of the C. elegans touch-cell
RT degenerin channel.";
RL Nature 420:669-673(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION.
RX PubMed=12385749; DOI=10.1016/s0925-4773(02)00293-9;
RA Zhang Y., Chalfie M.;
RT "MTD-1, a touch-cell-specific membrane protein with a subtle effect on
RT touch sensitivity.";
RL Mech. Dev. 119:3-7(2002).
CC -!- FUNCTION: Subunit of an amiloride-sensitive cation channel (degenerin
CC channel complex) permeable for sodium, potassium, lithium and N-
CC methylglucamine, and required for mechanosensory transduction (touch
CC sensitivity) (PubMed:12478294). Interacts with degenerin channel
CC proteins and stabilizes the channel (PubMed:12478294). Plays a role in
CC mechanosensory transduction (touch sensitivity) (PubMed:12478294,
CC PubMed:12385749). {ECO:0000269|PubMed:12385749,
CC ECO:0000269|PubMed:12478294}.
CC -!- SUBUNIT: Component of a non-voltage-gated amiloride-sensitive cation
CC channel complex (also called the degenerin channel complex) composed of
CC at least the mec-2, mec-4, mec-6 and mec-10 subunits; the complex
CC mediates mechanotransduction in touch cells (PubMed:12478294).
CC Interacts with mec-2, mec-4 and mec-10 (PubMed:12478294).
CC {ECO:0000269|PubMed:12478294}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12478294};
CC Single-pass membrane protein {ECO:0000269|PubMed:12478294}. Cell
CC projection, axon {ECO:0000269|PubMed:12478294}. Note=Co-localizes with
CC mec-4 in puncta along the axons of touch cell receptor neurons and in
CC muscles. {ECO:0000269|PubMed:12478294}.
CC -!- TISSUE SPECIFICITY: Expressed in neurons including the six touch
CC receptors, ventral cord motor neurons, HSN, PVD, PVC, IL1, and several
CC neurons near the nerve ring, in the anal ganglion and in the male tail
CC sensory rays, in muscles including the body wall, vulval, intestinal,
CC anal depressor and sphincter muscles, and in the excretory canal.
CC {ECO:0000269|PubMed:12478294}.
CC -!- PTM: Glycosylated. {ECO:0000269|PubMed:12478294}.
CC -!- SIMILARITY: Belongs to the paraoxonase family. {ECO:0000305}.
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DR EMBL; AF295927; AAL83724.1; -; mRNA.
DR EMBL; BX284601; CCD68986.1; -; Genomic_DNA.
DR PIR; T15204; T15204.
DR RefSeq; NP_491925.2; NM_059524.5.
DR AlphaFoldDB; O01811; -.
DR SMR; O01811; -.
DR BioGRID; 37837; 4.
DR STRING; 6239.W02D3.3; -.
DR TCDB; 1.A.6.2.2; the epithelial na(+) channel (enac) family.
DR EPD; O01811; -.
DR PaxDb; O01811; -.
DR PeptideAtlas; O01811; -.
DR EnsemblMetazoa; W02D3.3.1; W02D3.3.1; WBGene00003170.
DR EnsemblMetazoa; W02D3.3.2; W02D3.3.2; WBGene00003170.
DR EnsemblMetazoa; W02D3.3.3; W02D3.3.3; WBGene00003170.
DR GeneID; 172389; -.
DR UCSC; W02D3.3; c. elegans.
DR CTD; 172389; -.
DR WormBase; W02D3.3; CE31080; WBGene00003170; mec-6.
DR eggNOG; ENOG502S6UP; Eukaryota.
DR GeneTree; ENSGT00390000008932; -.
DR HOGENOM; CLU_049839_1_0_1; -.
DR InParanoid; O01811; -.
DR OMA; YEMMLLT; -.
DR OrthoDB; 888266at2759; -.
DR PhylomeDB; O01811; -.
DR Reactome; R-CEL-2142688; Synthesis of 5-eicosatetraenoic acids.
DR Reactome; R-CEL-9754706; Atorvastatin ADME.
DR PRO; PR:O01811; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00003170; Expressed in embryo and 3 other tissues.
DR GO; GO:0030424; C:axon; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004064; F:arylesterase activity; IEA:InterPro.
DR GO; GO:0005272; F:sodium channel activity; IEA:UniProtKB-KW.
DR GO; GO:0050976; P:detection of mechanical stimulus involved in sensory perception of touch; IMP:UniProtKB.
DR GO; GO:0007638; P:mechanosensory behavior; IMP:UniProtKB.
DR GO; GO:1905789; P:positive regulation of detection of mechanical stimulus involved in sensory perception of touch; IGI:UniProtKB.
DR GO; GO:1905792; P:positive regulation of mechanosensory behavior; IGI:UniProtKB.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR GO; GO:0009612; P:response to mechanical stimulus; IMP:WormBase.
DR Gene3D; 2.120.10.30; -; 1.
DR InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR InterPro; IPR002640; Arylesterase.
DR Pfam; PF01731; Arylesterase; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cell projection; Disulfide bond; Glycoprotein; Hydrolase;
KW Ion channel; Ion transport; Membrane; Neurodegeneration; Potassium;
KW Potassium transport; Reference proteome; Sodium; Sodium channel;
KW Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..377
FT /note="Mechanosensory abnormality protein 6"
FT /id="PRO_0000142347"
FT TOPO_DOM 1..13
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..377
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 94
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 48..369
FT /evidence="ECO:0000250"
FT MUTAGEN 235
FT /note="G->R: In u3; does not affect the expression levels.
FT Abolishes amiloride-sensitive current. Completely blocks
FT mec-4(d)-induced degenerations, and abolishes the punctate
FT expression of mec-4 in vivo."
FT /evidence="ECO:0000269|PubMed:12478294"
SQ SEQUENCE 377 AA; 42491 MW; B8DEBFE45474DAE6 CRC64;
MGLQSAAAHF INRFIIWITI FMVACFLLRL LVVLDLNKRV YNHTPGPCRV LTDNYKGTAG
MTYVESQKRV YITLGYGRAH DLKTKTGIAF YKTNRTDGRS QQEMYDLIEM TINWNGYEYK
KEFIPTGIDS YSSSNGRVLL YVINAHPNHQ CIHFFQIVES SKLNHRKAIC DPSFSSLQDI
AVVGPDRLFV TNMAAFGRGW AQILEFSLQT GQGAVYYYDG SKLSTAASSL IAPTGIGYDA
KRRILYVGSM IRESIFAYKV AKDTTLELLY EMMLLTSPIG VFVESKTGDI WIAAHPVIHE
SAWHYTHPEN QNIHSPSQIL RIRIQEEGNS WVTTEPYAND GATISASSSV VFTDEQMLIG
SSFGRLLHCD LTHSYIT
