ID   MECI_MAMSC              Reviewed;         123 AA.
AC   O54281; O54285;
DT   25-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Methicillin resistance regulatory protein MecI;
GN   Name=mecI;
OS   Mammaliicoccus sciuri (Staphylococcus sciuri).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Mammaliicoccus.
OX   NCBI_TaxID=1296;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Subsp. rodentium / K3, and Subsp. rodentium / K8;
RX   PubMed=9440511; DOI=10.1128/jb.180.2.236-242.1998;
RA   Wu S., de Lencastre H., Tomasz A.;
RT   "Genetic organization of the mecA region in methicillin-susceptible and
RT   methicillin-resistant strains of Staphylococcus sciuri.";
RL   J. Bacteriol. 180:236-242(1998).
CC   -!- FUNCTION: Transcriptional repressor that constitutively blocks the
CC       transcription of the gene for the penicillin-binding protein MecA.
CC       Binds DNA as a dimer (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Monomer and homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Upon exposure to beta-lactams, proteolytic cleavage at a single
CC       site impairs dimerization and abolishes repressor activity.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the BlaI transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; Y13095; CAA73540.1; -; Genomic_DNA.
DR   EMBL; Y13096; CAA73545.1; -; Genomic_DNA.
DR   AlphaFoldDB; O54281; -.
DR   SMR; O54281; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005650; BlaI_family.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR34134; PTHR34134; 1.
DR   Pfam; PF03965; Penicillinase_R; 1.
DR   PIRSF; PIRSF019455; CopR_AtkY; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Cytoplasm; DNA-binding; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..123
FT                   /note="Methicillin resistance regulatory protein MecI"
FT                   /id="PRO_0000062799"
FT   DNA_BIND        7..71
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          74..123
FT                   /note="Important for dimerization"
FT                   /evidence="ECO:0000250"
FT   SITE            101..102
FT                   /note="Cleavage"
FT                   /evidence="ECO:0000250"
FT   VARIANT         29..30
FT                   /note="NI -> YM (in strain: K8)"
FT   VARIANT         38
FT                   /note="R -> K (in strain: K8)"
SQ   SEQUENCE   123 AA;  14818 MW;  037504999FC12157 CRC64;
     MDNKTYEISS AEWEVMNIIW MKKYASANNI IEEIQMQRDW SPKTIRTLIT RLYKKGFIDR
     KKDNKIFQYY SLVEESDIKY KTSKNFINKV YKGGFNSLVL NFVEKEDLSQ DEIEELRNIL
     NKK