ID   MECI_STAAM              Reviewed;         123 AA.
AC   Q932L5;
DT   25-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Methicillin resistance regulatory protein MecI;
GN   Name=mecI; OrderedLocusNames=SAV0043;
OS   Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158878;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu50 / ATCC 700699;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
CC   -!- FUNCTION: Transcriptional repressor that constitutively blocks the
CC       transcription of the gene for the penicillin-binding protein MecA.
CC       Binds DNA as a dimer (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Monomer and homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Upon exposure to beta-lactams, proteolytic cleavage at a single
CC       site impairs dimerization and abolishes repressor activity.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the BlaI transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; BA000017; BAB56205.1; -; Genomic_DNA.
DR   RefSeq; WP_000369211.1; NC_002758.2.
DR   AlphaFoldDB; Q932L5; -.
DR   SMR; Q932L5; -.
DR   PaxDb; Q932L5; -.
DR   EnsemblBacteria; BAB56205; BAB56205; SAV0043.
DR   KEGG; sav:SAV0043; -.
DR   HOGENOM; CLU_119090_2_1_9; -.
DR   OMA; GNSREYY; -.
DR   PhylomeDB; Q932L5; -.
DR   BioCyc; SAUR158878:SAV_RS00350-MON; -.
DR   Proteomes; UP000002481; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005650; BlaI_family.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR34134; PTHR34134; 1.
DR   Pfam; PF03965; Penicillinase_R; 1.
DR   PIRSF; PIRSF019455; CopR_AtkY; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Cytoplasm; DNA-binding; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..123
FT                   /note="Methicillin resistance regulatory protein MecI"
FT                   /id="PRO_0000062796"
FT   DNA_BIND        7..71
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          74..123
FT                   /note="Important for dimerization"
FT                   /evidence="ECO:0000250"
FT   SITE            101..102
FT                   /note="Cleavage"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   123 AA;  14838 MW;  F4276DD78AD2C104 CRC64;
     MDNKTYEISS AEWEFMNIIW MKKYASANNI IEEIQMQKDW SPKTIRTLIT RLYKKGFIDR
     KKDNKIFQYY SLVEESDIKY KTSKNFINKV YKGGFNSLVL NFVEKEDLSQ DEIEELRNIL
     NKK