ID   MECI_STAAU              Reviewed;         123 AA.
AC   P68262; P26598;
DT   25-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Methicillin resistance regulatory protein MecI;
GN   Name=mecI;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BMS-1;
RX   PubMed=7911288; DOI=10.1128/aac.38.3.447;
RA   Archer G.L., Niemeyer D.M., Thanassi J.A., Pucci M.J.;
RT   "Dissemination among staphylococci of DNA sequences associated with
RT   methicillin resistance.";
RL   Antimicrob. Agents Chemother. 38:447-454(1994).
CC   -!- FUNCTION: Transcriptional repressor that constitutively blocks the
CC       transcription of the gene for the penicillin-binding protein MecA.
CC       Binds DNA as a dimer (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Monomer and homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Upon exposure to beta-lactams, proteolytic cleavage at a single
CC       site impairs dimerization and abolishes repressor activity.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the BlaI transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; L14020; AAA21183.1; -; Genomic_DNA.
DR   RefSeq; WP_000369214.1; NZ_WJUF01000012.1.
DR   AlphaFoldDB; P68262; -.
DR   SMR; P68262; -.
DR   GeneID; 64830977; -.
DR   OMA; GNSREYY; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005650; BlaI_family.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR34134; PTHR34134; 1.
DR   Pfam; PF03965; Penicillinase_R; 1.
DR   PIRSF; PIRSF019455; CopR_AtkY; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Cytoplasm; DNA-binding; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..123
FT                   /note="Methicillin resistance regulatory protein MecI"
FT                   /id="PRO_0000062798"
FT   DNA_BIND        7..71
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          74..123
FT                   /note="Important for dimerization"
FT                   /evidence="ECO:0000250"
FT   SITE            101..102
FT                   /note="Cleavage"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   123 AA;  14790 MW;  82FC1C109FD38410 CRC64;
     MDNKTYEISS AEWEVMNIIW MKKYASANNI IEEIQMQKDW SPKTIRTLIT RLYKKGFIDR
     KKDNKIFQYY SLVEESDIKY KTSKNFINKV YKGGFNSLVL NFVEKEDLSQ DEIEELRNIL
     NKK