ID   MED10_ASHGO             Reviewed;         151 AA.
AC   Q758M1;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 10;
DE   AltName: Full=Mediator complex subunit 10;
GN   Name=NUT2; Synonyms=MED10; OrderedLocusNames=AEL260C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 67.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 10 family.
CC       {ECO:0000305}.
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DR   EMBL; AE016818; AAS52425.2; -; Genomic_DNA.
DR   RefSeq; NP_984601.2; NM_209954.2.
DR   AlphaFoldDB; Q758M1; -.
DR   SMR; Q758M1; -.
DR   STRING; 33169.AAS52425; -.
DR   EnsemblFungi; AAS52425; AAS52425; AGOS_AEL260C.
DR   GeneID; 4620782; -.
DR   KEGG; ago:AGOS_AEL260C; -.
DR   eggNOG; KOG3046; Eukaryota.
DR   HOGENOM; CLU_096169_1_1_1; -.
DR   InParanoid; Q758M1; -.
DR   OMA; QYQRAKM; -.
DR   Proteomes; UP000000591; Chromosome V.
DR   GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR   GO; GO:0070847; C:core mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IEA:EnsemblFungi.
DR   InterPro; IPR019145; Mediator_Med10.
DR   Pfam; PF09748; Med10; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..151
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   10"
FT                   /id="PRO_0000303162"
SQ   SEQUENCE   151 AA;  17370 MW;  958E368D30956D86 CRC64;
     MDQQDLNKLQ TNLSDAEIRL ASIIESFIEL GVSVYDFPGT QESTQGMVTN LKRNVERIKL
     LNQHANDPDS QLRNMNIPLE VLQYIEDGRN PDVYTREFVE AIRRSNQYQR AKMHALKKLR
     DSLAEKIGEE FPDLVPQVQG IIERANGSRD K