MED10_DANRE
ID MED10_DANRE Reviewed; 134 AA.
AC Q0VIA1; A5WWF2; Q566Y7;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 10;
DE AltName: Full=Mediator complex subunit 10;
DE AltName: Full=Protein tennis match;
GN Name=med10; Synonyms=ten; ORFNames=si:dkey-262j19.1, zgc:112222;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=17208216; DOI=10.1016/j.ydbio.2006.11.034;
RA Lin X., Rinaldo L., Fazly A.F., Xu X.;
RT "Depletion of Med10 enhances Wnt and suppresses Nodal signaling during
RT zebrafish embryogenesis.";
RL Dev. Biol. 303:536-548(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Larva;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Negatively
CC regulates the Wnt signaling pathway and positively regulates the Nodal
CC signaling pathway. Required for cardiac cushion formation.
CC {ECO:0000250, ECO:0000269|PubMed:17208216}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0VIA1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0VIA1-2; Sequence=VSP_029613, VSP_029614;
CC -!- DEVELOPMENTAL STAGE: Expressed throughout embryogenesis.
CC {ECO:0000269|PubMed:17208216}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 10 family.
CC {ECO:0000305}.
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DR EMBL; AY698065; AAW56465.1; -; mRNA.
DR EMBL; CU013549; CAN88265.1; -; Genomic_DNA.
DR EMBL; CU013549; CAN88266.1; -; Genomic_DNA.
DR EMBL; BC093274; AAH93274.1; -; mRNA.
DR RefSeq; NP_001017699.1; NM_001017699.1.
DR AlphaFoldDB; Q0VIA1; -.
DR SMR; Q0VIA1; -.
DR STRING; 7955.ENSDARP00000094663; -.
DR PaxDb; Q0VIA1; -.
DR Ensembl; ENSDART00000103887; ENSDARP00000094663; ENSDARG00000070623. [Q0VIA1-1]
DR Ensembl; ENSDART00000145549; ENSDARP00000116070; ENSDARG00000070623. [Q0VIA1-2]
DR GeneID; 550394; -.
DR KEGG; dre:550394; -.
DR CTD; 84246; -.
DR ZFIN; ZDB-GENE-070117-2423; med10.
DR eggNOG; KOG3046; Eukaryota.
DR GeneTree; ENSGT00390000014501; -.
DR HOGENOM; CLU_096169_3_0_1; -.
DR InParanoid; Q0VIA1; -.
DR OMA; QYQRAKM; -.
DR OrthoDB; 1403581at2759; -.
DR PhylomeDB; Q0VIA1; -.
DR TreeFam; TF315096; -.
DR Reactome; R-DRE-212436; Generic Transcription Pathway.
DR PRO; PR:Q0VIA1; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 19.
DR Bgee; ENSDARG00000070623; Expressed in early embryo and 27 other tissues.
DR GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0036302; P:atrioventricular canal development; IMP:ZFIN.
DR GO; GO:1905072; P:cardiac jelly development; IMP:ZFIN.
DR GO; GO:0007507; P:heart development; IMP:ZFIN.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR019145; Mediator_Med10.
DR Pfam; PF09748; Med10; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; Developmental protein; Nucleus;
KW Reference proteome; Transcription; Transcription regulation;
KW Wnt signaling pathway.
FT CHAIN 1..134
FT /note="Mediator of RNA polymerase II transcription subunit
FT 10"
FT /id="PRO_0000303155"
FT VAR_SEQ 104..106
FT /note="KFK -> RMV (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_029613"
FT VAR_SEQ 107..134
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_029614"
FT CONFLICT 44
FT /note="M -> V (in Ref. 2; AAH93274)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 134 AA; 15438 MW; 704E7EC60167A3CB CRC64;
MAEKFDNLEE HLEKFVENIR QLGIIVSDFQ PSSQTGLNQK LNFMITGLQD IEKCRQQLHD
INVPLEVFEY IDQGRNPQLY TKECLERALA KNEQVKGKID TLTKFKSLLI SELGKVFPEE
MAKYKAIHGD DPPS
